Hydrogen fermentation of food waste by alkali-shock pretreatment: microbial community analysis and limitation of continuous operation.

In the study, at first, batch tests were performed to investigate the effect of alkali-shock on H2 production from food waste (FW). After alkali-pretreatment of FW at pH 9.0-13.0, the FW was cultivated under mesophilic condition at pH 6.0 for 30 h without external inoculum addition. The amount of H2 production from FW pretreated at pH 11.0 and 12.0 was higher than that achieved in other pretreatment pH. The main metabolite was butyrate, and Clostridium were dominant at pH 11.0 and 12.0. Meanwhile, lactate was the main metabolite with Enterococcus and Streptococcus being the dominant genus at other pretreatment pH. When the batch process was switched to a continuous mode, H2 production was significantly dropped due to the increased activity of H2-consumers. The reliability of alkali-pretreatment at pH 11.0 was proven by repeating the scale-up batch process, recording 1.57±0.11 mol H2/mol hexose(added) (17±2LH2/kg FW) and 4.39±0.32LH2/L/d.

[1]  Hang-Sik Shin,et al.  Effects of base-pretreatment on continuous enriched culture for hydrogen production from food waste , 2008 .

[2]  Hang-Sik Shin,et al.  Start-up strategy for continuous fermentative hydrogen production : Early switchover from batch to continuous operation , 2008 .

[3]  F. Smith,et al.  COLORIMETRIC METHOD FOR DETER-MINATION OF SUGAR AND RELATED SUBSTANCE , 1956 .

[4]  Zhiyan He,et al.  Survival of Enterococcus faecalis during alkaline stress: changes in morphology, ultrastructure, physiochemical properties of the cell wall and specific gene transcripts. , 2013, Archives of oral biology.

[5]  Hang-Sik Shin,et al.  Hydrogen fermentation of food waste without inoculum addition , 2009 .

[6]  R. Song,et al.  Coupling of the hydrogen and polyhydroxyalkanoates (PHA) production through anaerobic digestion from Taihu blue algae. , 2010, Bioresource technology.

[7]  A. Fodor,et al.  Molecular Diversity of a North Carolina Wastewater Treatment Plant as Revealed by Pyrosequencing , 2008, Applied and Environmental Microbiology.

[8]  Samir Kumar Khanal,et al.  Kinetic study of biological hydrogen production by anaerobic fermentation , 2006 .

[9]  D. Cliver,et al.  Microorganisms in honey. , 1996, International journal of food microbiology.

[10]  Qi Zhou,et al.  Enhancement of bioenergy production from organic wastes by two-stage anaerobic hydrogen and methane production process. , 2011, Bioresource technology.

[11]  W. Ng,et al.  Bioconversion of food waste to energy : a review , 2014 .

[12]  Janez Levec,et al.  Catalytic surface development of novel nickel plate catalyst with combined thermally annealed platinum and alumina coatings for steam methane reforming , 2013 .

[13]  J. Lalman,et al.  Elucidating acetogenic H2 consumption in dark fermentation using flux balance analysis. , 2013, Bioresource technology.

[14]  Hang-Sik Shin,et al.  Experience of a pilot-scale hydrogen-producing anaerobic sequencing batch reactor (ASBR) treating food waste , 2010 .

[15]  V. Vavilin,et al.  Modelling hydrogen partial pressure change as a result of competition between the butyric and propionic groups of acidogenic bacteria , 1995 .

[16]  E. Rorman,et al.  Rapid identification of Enterobacter hormaechei and Enterobacter cloacae genetic cluster III , 2014, Journal of applied microbiology.

[17]  N. Pisutpaisal,et al.  Production of hydrogen and methane by one and two stage fermentation of food waste , 2013 .

[18]  H. Drake,et al.  Ecological consequences of the phylogenetic and physiological diversities of acetogens , 2002, Antonie van Leeuwenhoek.

[19]  Hang-Sik Shin,et al.  Natural inducement of hydrogen from food waste by temperature control , 2011 .

[20]  Mi‐Sun Kim,et al.  Effect of the accuracy of pH control on hydrogen fermentation. , 2015, Bioresource technology.

[21]  Sangeun Oh,et al.  The relative effectiveness of pH control and heat treatment for enhancing biohydrogen gas production. , 2003, Environmental science & technology.

[22]  Mi‐Sun Kim,et al.  Alkali-treated sewage sludge as a seeding source for hydrogen fermentation of food waste leachate , 2013 .

[23]  Dong-Hoon Kim,et al.  Hydrogenases for biological hydrogen production. , 2011, Bioresource technology.

[24]  E. Arnold,et al.  Standard methods for the examination of water and wastewater. 16th ed. , 1985 .

[25]  Hang-Sik Shin,et al.  Effect of gas sparging on continuous fermentative hydrogen production , 2006 .

[26]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[27]  Hang-Sik Shin,et al.  Optimization of continuous hydrogen fermentation of food waste as a function of solids retention time independent of hydraulic retention time , 2008 .

[28]  Y. Oh,et al.  Application of a novel enzymatic pretreatment using crude hydrolytic extracellular enzyme solution to microalgal biomass for dark fermentative hydrogen production. , 2014, Bioresource technology.

[29]  Brett I. Pletschke,et al.  Food processing waste: Problems, current management and prospects for utilisation of the lignocellulose component through enzyme synergistic degradation , 2013 .

[30]  Magdalena Frąc,et al.  Methane fermentation process for utilization of organic waste , 2012 .

[31]  K. A. Gilles,et al.  COLORIMETRIC METHOD FOR DETER-MINATION OF SUGAR AND RELATED SUBSTANCE , 1956 .

[32]  W. Holzapfel,et al.  Lactic acid bacteria of foods and their current taxonomy. , 1997, International journal of food microbiology.

[33]  R. Dinsdale,et al.  Continuous fermentative hydrogen production from a wheat starch co‐product by mixed microflora , 2003, Biotechnology and bioengineering.

[34]  P. Lagasse,et al.  Production of class A biosolids with anoxic low dose alkaline treatment and odor management. , 2004, Water science and technology : a journal of the International Association on Water Pollution Research.