Synthesis and Anticancer Activity of Certain Fused Pyridazine Derivatives

Novel series of pyridazine containing compounds were synthesized through the reaction of N-carbamimidoyl-4-[(6 chloropyridazin-3-yl) amino]benzenesulphonamide 1 with either 2-aminophenol, acetophenone phenylhydrazone derivatives, anthranilic acid derivatives, thiosemicarbazide or substituted benzoyl hydrazide to afford the corresponding derivatives 2, 3a-c, 4a-d, 5 and 6a-c respectively. The structure of the newly synthesized compounds was characterized by spectroscopic means and elemental analysis. All the synthesized compounds were screened for their anticancer activity in vitro on colon cancer cell line (HCT-116) and breast cancer cell line (MCF-7). Compound 4a showed potent anticancer activity against colon cancer cell line (HCT-116) with IC50 11.90 μM in comparison to imatinib (Gleevec ®).