Microsphere-Based Multiplex Immunoassays: Development and Applications Using Luminex® xMAP® Technology

This chapter describes the Luminex range of bead-based multiplex assays, which are based on flow cytometry. These are widely used by third party manufacturers and individual researchers to develop multiplexed immunoassays for up to 500 different analytes simultaneously. The equipment and reagents available are described and the theory explained. This chapter includes detailed information to enable assays to be developed using the core system, including buffer formulations and their applications. Step-by-step protocols are provided on coupling of proteins to microspheres, confirmation of antibody coupling, and the final immunoassay (immunometric, indirect, competitive and combined immunometric/competitive). Notes are also provided on assay optimization. A list of commercially available products from third-party manufacturers is included.

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