Lactobacilli binding human A-antigen expressed in intestinal mucosa.

Adherent lactic acid bacteria (LAB) in the human intestine were investigated using the surface plasmon resonance technique with the biosensor BIACORE-1000. Ninety-three LAB strains were isolated from human feces and evaluated for binding to human blood type-A antigen [GalNAcalpha1-3 (Fucalpha1-2) Gal-: A-trisaccharide] expressed in the intestinal mucosa. Eleven strains showed strong adherence to an A-trisaccharide biotinyl polymer (BP) probe, and slightly or no adherence to a B-trisaccharide BP probe. Four strains with high adherence (high A/B ratio) were selected and their surface layer proteins (SLPs) were evaluated for A-antigen ligand binding using BIACORE. The SLP from L. brevis strain OLL2772 showed a single band at ca. 48 kDa by SDS-PAGE analysis and it had a very strong adherence to the human A-antigen, as shown using an anti-A lectin blocking technique. A partial N-terminal sequence of the band showed strong homology to an S-layer protein of L. brevis ATCC8287T. The probiotic LAB binds to human blood type-A antigen expressed in the intestinal mucosa which may aid in colonization of the gut.

[1]  T. Saito,et al.  New binding assay and preparative trial of cell-surface lectin from Lactobacillus acidophilus group lactic acid bacteria. , 1999, Journal of dairy science.

[2]  J. Sillanpää,et al.  Characterization of the Collagen-Binding S-Layer Protein CbsA of Lactobacillus crispatus , 2000, Journal of bacteriology.

[3]  V. Ocaña,et al.  Adhesion ability of Lactobacillus to vaginal epithelial cells: study by microbiological methods. , 2004, Methods in molecular biology.

[4]  G. Bergonzelli,et al.  Cell Surface-Associated Elongation Factor Tu Mediates the Attachment of Lactobacillus johnsonii NCC533 (La1) to Human Intestinal Cells and Mucins , 2004, Infection and Immunity.

[5]  H. Devaraj,et al.  Adherence of Shigella dysenteriae 1 to Human Colonic Mucin , 2001, Current Microbiology.

[6]  R. Pizarro,et al.  Adhesion of probiotic lactobacilli to chick intestinal mucus. , 2003, Canadian journal of microbiology.

[7]  T. Honda,et al.  Histochemical demonstration of sugar residues by lectin and immunocytochemical techniques for blood group antigens in human colon , 1987, The Histochemical Journal.

[8]  P. Fratamico,et al.  Detection of Escherichia coli 0157:H7 using a surface plasmon resonance biosensor , 1998 .

[9]  T. Mukai,et al.  Lectin-like activity of Lactobacillus acidophilus strain JCM 1026. , 1992, FEMS microbiology letters.

[10]  P. Edman,et al.  A method for the determination of amino acid sequence in peptides. , 1949, Archives of biochemistry.

[11]  D. O'Shannessy,et al.  Immobilization chemistries suitable for use in the BIAcore surface plasmon resonance detector. , 1992, Analytical biochemistry.

[12]  M. Blaser,et al.  Functional Adaptation of BabA, the H. pylori ABO Blood Group Antigen Binding Adhesin , 2004, Science.

[13]  A. P. DE Ruiz Holgado,et al.  Study of the morphology of the cell walls of some strains of lactic acid bacteria and related species. , 1998, Journal of food protection.

[14]  S. Salminen,et al.  Adherence of Probiotic Bacteria to Human Intestinal Mucus in Healthy Infants and during Rotavirus Infection , 2001, Clinical Diagnostic Laboratory Immunology.

[15]  Surface Display of the Receptor-Binding Region of the Lactobacillusbrevis S-Layer Protein in Lactococcuslactis Provides Nonadhesive Lactococci with the Ability To Adhere to Intestinal Epithelial Cells , 2003, Applied and Environmental Microbiology.

[16]  Kazuya Saito,et al.  A New Assay Using Surface Plasmon Resonance (SPR) to Determine Binding of the Lactobacillus acidophilus Group to Human Colonic Mucin , 2004, Bioscience, biotechnology, and biochemistry.

[17]  Stephen D Holmes,et al.  Studies on the interaction of staphylococcus aureus and staphylococcus epidermidis with fibronectin using surface plasmon resonance (BIAcore) , 1997 .

[18]  H. Jonsson,et al.  A high-molecular-mass cell-surface protein from Lactobacillus reuteri 1063 adheres to mucus components. , 2002, Microbiology.

[19]  M. De Felice,et al.  Small surface-associated factors mediate adhesion of a food-isolated strain of Lactobacillus fermentum to Caco-2 cells. , 2005, Research in microbiology.

[20]  F. Smith,et al.  A Colorimetric Method for the Determination of Sugars , 1951, Nature.

[21]  M. Kirschner,et al.  Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. , 1977, The Journal of biological chemistry.

[22]  D. Graham,et al.  Norwalk virus infection and disease is associated with ABO histo-blood group type. , 2002, The Journal of infectious diseases.

[23]  S. Salminen,et al.  The ability of probiotic bacteria to bind to human intestinal mucus. , 1998, FEMS microbiology letters.