Diagnostic value of molecular forms of prostate-specific antigen for female breast cancer.

OBJECTIVES To examine the diagnostic value of prostate-specific antigen (PSA) subfractions in the serum of female breast cancer patients. DESIGN AND METHODS PSA subfractions (free PSA, F-PSA; PSA bound to alpha 1-antichymotrypsin, PSA-ACT) were determined in the serum of patients with breast cancer and in the serum of healthy women. Serum was injected into a high-performance liquid chromatography column and all fractions were analyzed for PSA using a highly sensitive PSA immunofluorometric assay. We studied 3 normal male sera, 3 sera from prostate cancer patients who underwent radical prostatectomy (all for comparative purposes), 3 sera from healthy women, 3 sera from women with breast cancer obtained presurgically, and 7 sera from women with breast cancer, postsurgically. RESULTS All male sera contained mostly PSA-ACT complexes and very little free PSA. Sera from all healthy women also contained mostly PSA-ACT complexes and nondetectable or traces of free PSA. All 3 presurgical sera from patients with breast cancer contained predominantly free PSA. Patients who had surgical resection of the breast tumor and were in remission had postsurgical serum PSA subfractions similar to those of healthy women (i.e., mostly PSA-ACT complexes). CONCLUSION The serum PSA subfractions of breast cancer patients are substantially different from serum PSA subfractions of male patients, healthy females, and females who have apparently been treated successfully for breast cancer. These findings may form the basis for a serological diagnostic test for breast cancer.

[1]  U. Stenman,et al.  A complex between prostate-specific antigen and alpha 1-antichymotrypsin is the major form of prostate-specific antigen in serum of patients with prostatic cancer: assay of the complex improves clinical sensitivity for cancer. , 1991, Cancer research.

[2]  E. Diamandis,et al.  Prostate-specific antigen in amniotic fluid of normal and abnormal pregnancies. , 1996, Clinical biochemistry.

[3]  E. Diamandis,et al.  Immunoreactive prostate‐specific antigen in lung tumors , 1995, Journal of clinical laboratory analysis.

[4]  E. Diamandis,et al.  Original Articles: Prostate Cancer: Measurement of Serum Prostate Specific Antigen Levels in Women and in Prostatectomized Men With an Ultrasensitive Immunoassay Technique , 1995 .

[5]  J. Oesterling,et al.  Prostate specific antigen: a critical assessment of the most useful tumor marker for adenocarcinoma of the prostate. , 1991, The Journal of urology.

[6]  E. Diamandis,et al.  Prostate-specific antigen immunoreactivity in amniotic fluid. , 1995, Clinical chemistry.

[7]  E. Diamandis,et al.  Oral Contraceptive-induced Expression of Prostate-specific Antigen in the Female Breast (*) , 1995, The Journal of Biological Chemistry.

[8]  J. Krieken.  Prostate Marker Immunoreactivity in Salivary Gland Neoplasms: A Rare Pitfall in Immunohistochemistry , 1993 .

[9]  P. Sismondi,et al.  Prostate-specific antigen is a new favorable prognostic indicator for women with breast cancer. , 1995, Cancer research.

[10]  J. Oesterling,et al.  Molecular forms of prostate-specific antigen and the human kallikrein gene family: a new era. , 1995, Urology.

[11]  E. Diamandis,et al.  Ultrasensitive detection of prostate-specific antigen by a time-resolved immunofluorometric assay and the Immulite immunochemiluminescent third-generation assay: potential applications in prostate and breast cancers. , 1996, Clinical chemistry.

[12]  E. Diamandis,et al.  Immunoreactive prostate-specific antigen levels in female and male breast tumors and its association with steroid hormone receptors and patient age. , 1994, Clinical biochemistry.

[13]  D. Poller.  Prostate marker immunoreactivity in salivary gland neoplasms. , 1994, The American journal of surgical pathology.

[14]  H. Lilja,et al.  Enzymatic activity of prostate-specific antigen and its reactions with extracellular serine proteinase inhibitors. , 1990, European journal of biochemistry.

[15]  D. Katsaros,et al.  Prostate-specific antigen in serum of women with breast cancer. , 1995, British Journal of Cancer.

[16]  D. Armbruster,et al.  Prostate-specific antigen: biochemistry, analytical methods, and clinical application. , 1993, Clinical chemistry.

[17]  C. Croce,et al.  Molecular characterization of prostate-specific antigen messenger RNA expressed in breast tumors. , 1994, Cancer research.

[18]  E. Diamandis,et al.  Prostate-specific antigen in milk of lactating women. , 1995, Clinical chemistry.

[19]  E. Diamandis,et al.  New biological functions of prostate-specific antigen? , 1995, The Journal of clinical endocrinology and metabolism.