A novel microgravimetric DNA sensor with high sensitivity.

A novel method using an amplifier with a cantilever and gold nanoparticles successfully to extend the length of the target for the specific and high sensitive detection of DNA was reported. When the size of gold nanoparticle is 50 nm, a sensitivity of 10(-15)M for the single base mutation detection has been achieved.

[1]  T. M. Herne,et al.  Observation of Hybridization and Dehybridization of Thiol-Tethered DNA Using Two-Color Surface Plasmon Resonance Spectroscopy , 1997 .

[2]  J. Storhoff,et al.  A DNA-based method for rationally assembling nanoparticles into macroscopic materials , 1996, Nature.

[3]  Tao Liu,et al.  Particle size effect of the DNA sensor amplified with gold nanoparticles , 2002 .

[4]  C. Mirkin,et al.  Homogeneous, Nanoparticle-Based Quantitative Colorimetric Detection of Oligonucleotides , 2000 .

[5]  Michael J. Natan,et al.  Kinetic Control of Interparticle Spacing in Au Colloid-Based Surfaces: Rational Nanometer-Scale Architecture , 1996 .

[6]  Lin Lin,et al.  Enhancement of the immobilization and discrimination of DNA probe on a biosensor using gold nanoparticles , 2001 .

[7]  George C Schatz,et al.  What controls the melting properties of DNA-linked gold nanoparticle assemblies? , 2000, Journal of the American Chemical Society.

[8]  I. Willner,et al.  Amplified Microgravimetric Quartz-Crystal-Microbalance Assay of DNA Using Oligonucleotide-Functionalized Liposomes or Biotinylated Liposomes , 2000 .

[9]  K. Gotoh,et al.  Detachment behavior of Langmuir-Blodgett films of arachidic acid from a gold surface studied by the quartz crystal microbalance method , 2002 .

[10]  I. Willner,et al.  Electrochemical and quartz crystal microbalance detection of the cholera toxin employing horseradish peroxidase and GM1-functionalized liposomes. , 2001, Analytical chemistry.

[11]  X. Zhou,et al.  Microgravimetric DNA sensor based on quartz crystal microbalance: comparison of oligonucleotide immobilization methods and the application in genetic diagnosis. , 2001, Biosensors & bioelectronics.

[12]  C. Mirkin,et al.  Two-color labeling of oligonucleotide arrays via size-selective scattering of nanoparticle probes. , 2001, Journal of the American Chemical Society.

[13]  A. Bard,et al.  Immobilization and Hybridization of DNA on an Aluminum(III) Alkanebisphosphonate Thin Film with Electrogenerated Chemiluminescent Detection , 1995 .

[14]  T. Vo‐Dinh,et al.  Application of an Antibody Biochip for p53 Detection and Cancer Diagnosis , 2001, Biotechnology progress.

[15]  S. Nguyen,et al.  DNA-block copolymer conjugates. , 2001, Journal of the American Chemical Society.

[16]  M. Inganäs,et al.  Prognostic value of P53 gene mutations in a large series of node-negative breast cancer patients. , 1998, Cancer research.

[17]  I. Willner,et al.  Probing of DNA and Single-Base Mismatches by Chemical Force Microscopy Using Peptide Nucleic Acid-Modified Sensing Tips and Functionalized Surfaces , 2001 .

[18]  Robert L. Letsinger,et al.  The DNA-Mediated Formation of Supramolecular Mono- and Multilayered Nanoparticle Structures , 2000 .

[19]  Long Jiang,et al.  Sensitivity enhancement of DNA sensors by nanogold surface modification. , 2002, Biochemical and biophysical research communications.