DNA Recombination: Holliday Junctions Dynamics and Branch Migration

these models using a Holliday junction undergoing branch migration and time-lapse atomic force microscopy (AFM), an capable of DNA The single molecule AFM experiments performed in the presence and in the of divalent cations show that mobile Holliday junctions adopt an unfolded conformation during branch migration that is retained despite a broad range of motion in the junction’s arms. This conformation of the junction remains unchanged until strand separation. The data obtained support the model for branch having the extended conformation of the junction.