The Effect of Dietary Saccharoculture on Growth Performance, Non-Specific Immunity and Autochthonous Gut Microbiota of Gibel Carp Carassius auratus

The objective of this study was to evaluate the effect of a commercial fermented yeast product (Saccharoculture, Veterinary Pharmaceuticals, Korea), on the growth performance, non-specific immunity and autochthonous intestinal microbiota of gibel carp Carassius auratus. Following an eight week feeding trial the growth performance and serum non-specific immunity of fish fed one of four treatments (A: control group, B: 2 g/kg Saccharoculture, C: 3 g/kg Saccharoculture or D: 2 g/kg Saccharoculture + 0.1 g/kg flavomycin) was measured. Additionally, the predominant autochthonous intestinal microbiota was analyzed by denaturing gradient gel electrophoresis (DGGE). The results indicated that dietary supplementation of Saccharoculture or Saccharoculture combined with flavomycin significantly improved final weight, weight gain (WG), special growth rate (SGR) and feed conversion ratio (FCR) compared to the control group (P 0.05). Compared to the control group, fish fed dietary yeast exhibited modulated intestinal bacterial communities. Levels of some bacteria were elevated by dietary Saccharoculture, such as, Acinetobacter sp. (FR749840.1, FJ646641.1), Escherichia vulneris (HQ259947.1) and Cetobacterium somerae (AB353124.1). Cetobacterium sp. (HM778168.1) and Lactococcus lactis subsp. (EF589778.1) were suppressed in the Saccharoculture with flavomycin group. However, Nevskia sp. (AB426558.1) was suppressed by the higher level Saccharoculture or Saccharoculture and flavomycin combination.

[1]  Yun‐Zhang Sun,et al.  Single or combined effects of fructo‐ and mannan oligosaccharide supplements and Bacillus clausii on the growth, feed utilization, body composition, digestive enzyme activity, innate immune response and lipid metabolism of the Japanese flounder Paralichthys olivaceus , 2011 .

[2]  S. Hoseinifar,et al.  The effects of dietary inactive brewer's yeast Saccharomyces cerevisiae var. ellipsoideus on the growth, physiological responses and gut microbiota of juvenile beluga (Huso huso) , 2011 .

[3]  K. Meng,et al.  Effects of the antibiotic growth promoters flavomycin and florfenicol on the autochthonous intestinal microbiota of hybrid tilapia (Oreochromis niloticus ♀ × O. aureus ♂) , 2010, Archives of Microbiology.

[4]  I. Yoon,et al.  Effects of dietary Saccharomyces cerevisiae fermentation product (DVAQUA®) on growth performance, intestinal autochthonous bacterial community and non-specific immunity of hybrid tilapia (Oreochromis niloticus ♀×O. aureus ♂) cultured in cages , 2009 .

[5]  J. Celli,et al.  Of microbes and membranes: pathogenic subversion of host cell processes. , 2008, Cell host & microbe.

[6]  M. Abdel‐Tawwab,et al.  Evaluation of commercial live bakers' yeast, Saccharomyces cerevisiae as a growth and immunity promoter for Fry Nile tilapia, Oreochromis niloticus (L.) challenged in situ with Aeromonas hydrophila , 2008 .

[7]  S. Aly,et al.  Studies on Bacillus subtilis and Lactobacillus acidophilus, as potential probiotics, on the immune response and resistance of Tilapia nilotica (Oreochromis niloticus) to challenge infections. , 2008, Fish & shellfish immunology.

[8]  B. Yao,et al.  Effect of intraperitoneal injection of immunostimulatory substances on allochthonous gut microbiota of Atlantic salmon (Salmo salar L.) determined using denaturing gradient gel electrophoresis , 2008 .

[9]  H. Sugita,et al.  Novel ecological niche of Cetobacterium somerae, an anaerobic bacterium in the intestinal tracts of freshwater fish , 2007, Letters in applied microbiology.

[10]  R. Santhanam,et al.  Influence of Probiotics on the Growth and Gut Microbial Load of Juvenile Goldfish (Carassius auratus) , 2004, Asian Fisheries Science.

[11]  Young Cheol Kim,et al.  Degradation of acyl-homoserine lactone molecules by Acinetobacter sp. strain C1010. , 2004, Canadian journal of microbiology.

[12]  F. Haesebrouck,et al.  Antimicrobial Growth Promoters Used in Animal Feed: Effects of Less Well Known Antibiotics on Gram-Positive Bacteria , 2003, Clinical Microbiology Reviews.

[13]  M. A. Olvera‐Novoa,et al.  Use of the bacteria Streptococcus faecium and Lactobacillus acidophilus, and the yeast Saccharomyces cerevisiae as growth promoters in Nile tilapia (Oreochromis niloticus) , 2003 .

[14]  M. Médici,et al.  Interaction of lactic acid bacteria with the gut immune system , 2002, European Journal of Clinical Nutrition.

[15]  M. Xue,et al.  Effect of several feeding stimulants on diet preference by juvenile gibel carp (Carassius auratus gibelio), fed diets with or without partial replacement of fish meal by meat and bone meal , 2001 .

[16]  J. Meseguer,et al.  Immunomodulatory effects of dietary intake of chitin on gilthead seabream (Sparus aurata L.) innate immune system. , 2001, Fish & shellfish immunology.

[17]  E. Ringø,et al.  INTESTINAL MICROFLORA OF FISH LARVAE AND FRY , 1999 .

[18]  John D Lambris,et al.  Evolution and diversity of the complement system of poikilothermic vertebrates , 1998, Immunological reviews.

[19]  A. Sundsfjord,et al.  The effect of dietary fatty acids on lactic acid bacteria associated with the epithelial mucosa and from faecalia of Arctic charr, Salvelinus alpinus (L.) , 1998, Journal of applied microbiology.

[20]  T. Fujita,et al.  Complement-related serine proteases in tunicates and vertebrates. , 1998, Current opinion in immunology.

[21]  E. Ringø,et al.  Microflora of Arctic charr, Salvelinus alpinus (L.): gastrointestinal microflora of free‐living fish and effect of diet and salinity on intestinal microflora , 1994 .

[22]  R. Winfree,et al.  Nutritional value of dietary nucleic acids and purine bases to rainbow trout (Oncorhynchus mykiss) , 1992 .

[23]  B. Robertsen,et al.  Yeast glucan induces increase in lysozyme and complement-mediated haemolytic activity in Atlantic salmon blood , 1992 .

[24]  I. Yoon,et al.  Effects of dietary antibiotic growth promoter and Saccharomyces cerevisiae fermentation product on production, intestinal bacterial community, and nonspecific immunity of hybrid tilapia (Oreochromis niloticus female x Oreochromis aureus male). , 2011, Journal of animal science.

[25]  W. Khalil,et al.  Effect of yeast (Saccharomyces cerevisiae) on reduction of aflatoxicosis, enhancement of growth performance and expression of neural and gonadal genes in Japanese quail , 2010 .

[26]  A. Hajimoradloo,et al.  Effect of using yeast ( Saccharomyces cerevisiae ) as probiotic on growth parameters, survival and carcass quality in rainbow trout Oncorhynchus mykiss fry , 2009 .

[27]  J. Raa.  The use of immunostimulatory substances in fish and shellfish farming , 1996 .

[28]  D. K. Sakai.  Repertoire of complement in immunological defense mechanisms of fish , 1992 .