Production tactic and physiochemical properties of low ω6/ω3 ratio structured lipid synthesised from perilla and soybean oil

Summary Structured lipid (SL) was synthesised by enzymatic interesterification of soybean oil and perilla oil. Response surface methodology (RSM) was used to determine the effects of three variables on the lipase-catalysed interesterification. Based on ridge analysis, combination of reaction time (X1; 18 h), reaction temperature (X2; 60 °C), and substrate mole ratio (X3; 1:1) were optimised for higher incorporation of ω3 (alpha (α) linolenic acid (ALA)) (Y). The predictive model was found to be adequate due to no significant lack of fit and satisfactory level of coefficient of determination (R2 = 0.97). Experiments were conducted under optimised conditions which were predicted by the model equations, obtained from RSM yielded SL with linoleic (44.01%) and ALA (35.82%) were detected at sn-2 position. The effects of antioxidants such as catechin, butylated hydroxytoluene (BHT) BHT and rosemary extracted on the oxidative stability in SL were investigated. Among all antioxidants, the highest stability was obtained from catechin.

[1]  C. Akoh,et al.  Scaled-up production of zero-trans margarine fat using pine nut oil and palm stearin. , 2010 .

[2]  C. Akoh,et al.  Lipase-catalyzed production of solid fat stock from fractionated rice bran oil, palm stearin, and conjugated linoleic acid by response surface methodology , 2008 .

[3]  P. Pristovsek,et al.  Green tea catechins inhibit bacterial DNA gyrase by interaction with its ATP binding site. , 2007, Journal of medicinal chemistry.

[4]  A. Simopoulos.  Evolutionary aspects of diet, the omega-6/omega-3 ratio and genetic variation: nutritional implications for chronic diseases. , 2006, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.

[5]  C. Akoh,et al.  Antioxidant evaluation and oxidative stability of structured lipids from extravirgin olive oil and conjugated linoleic acid. , 2006, Journal of agricultural and food chemistry.

[6]  J. Tsaknis,et al.  Extraction and identification of natural antioxidant from Sideritis euboea (mountain tea). , 2005, Journal of agricultural and food chemistry.

[7]  S. Chugh,et al.  Fish oil supplementation and risk of ventricular tachycardia and ventricular fibrillation in patients with implantable defibrillators: a randomized controlled trial. , 2005, JAMA.

[8]  B. Özçelik,et al.  Enrichment of hazelnut oil with long-chain n−3 PUFA by lipase-catalyzed acidolysis: Optimization by response surface methodology , 2005 .

[9]  Ki-Teak Lee,et al.  Chemical and Volatile Characterization of Structured Lipid from Soybean Oil Containing Conjugated Linoleic Acid , 2003 .

[10]  Duo Li,et al.  What is the role of α-linolenic acid for mammals? , 2002, Lipids.

[11]  A. Simopoulos,et al.  The importance of the ratio of omega-6/omega-3 essential fatty acids. , 2002, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.

[12]  M. Ramírez,et al.  Absorption and distribution of dietary fatty acids from different sources. , 2001, Early human development.

[13]  R. Goldbohm,et al.  Plant sterol intakes and colorectal cancer risk in the Netherlands Cohort Study on Diet and Cancer. , 2001, The American journal of clinical nutrition.

[14]  Y. Kim,et al.  Modulation of Liver Microsomal Monooxygenase System by Dietary n-6/n-3 Ratios in Rat Hepatocarcinogenesis , 2000, Nutrition and cancer.

[15]  Sang-Hyun Kim,et al.  Inhibitory Effect of Mast Cell-Mediated Immediate-Type Allergic Reactions in Rats by Perilla Frutescens , 2000, Immunopharmacology and immunotoxicology.

[16]  H. Umekawa,et al.  Comparative effects of safflower oil and perilla oil on serum and hepatic lipid levels, fatty acid compositions of serum and hepatic phospholipids, and hepatic mRNA expressions of 3-hydroxy-3-methylglutaryl CoA reductase, LDL receptor, and cholesterol 7alpha-hydroxylase in young and adult rats. , 2000 .

[17]  T. Sanders,et al.  Influence of n-6 versus n-3 polyunsaturated fatty acids in diets low in saturated fatty acids on plasma lipoproteins and hemostatic factors. , 1997, Arteriosclerosis, thrombosis, and vascular biology.

[18]  Hyo-Sun Shin,et al.  Lipid composition of perilla seed , 1994 .

[19]  F. Leighton,et al.  Flavonoids as stabilizers of fish oil: An alternative to synthetic antioxidants , 1993 .