Diel and depth profiles of DNA photodamage in bacterioplankton exposed to ambient solar ultraviolet radiation

Bacteria play a central role in the cycling of nutrients and energy flow to higher trophic levels, yet the effects of ultraviolet-B (UV-B) radiation upon bacterioplankton have been largely overlooked. Using a highly specific radloimmunoassay, measurements of solar-induced DNA photodamage (cyclobutane pyriinldine dimers) were taken in planktonic samples collected from the northern Gulf of Ilcxico. Diel patterns of dimer accumulation and repair were observed in both the bacterloplankton ~ 1 7 1 ' fraction ( 0.8 pm < 120 pm), although damacje induction was approximately twice as much in the bacterioplankton fraction. Depth profiles of Dii \ damage in the bacterioplankton size fraction during cdlnl and moderate seas demonstrated the infl~iencc ot mixlng on t h e distribution of W radiation effects. During calm seas, damage was greatest in surface waters, decreased with depth, and could be detected to 10 m. In moderate seas, however, no net accumulation of damage was observed, even at the surface. The results demonstrate that bacteria are more susceptible to UV-B damage and may wrve as a more sensitive indicator of UV stress than other microorganisms. Wave action and mixing strongly influence the effects of UV-B in surface waters, demonstl-ating that UV-B effects may not always be predictable from measures of UV radiat io~ attenuation.

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