Comparison of growth, acetate production, and acetate inhibition of Escherichia coli strains in batch and fed-batch fermentations

The growth characteristics and acetate production of several Escherichia coli strains were compared by using shake flasks, batch fermentations, and glucose-feedback-controlled fed-batch fermentations to assess the potential of each strain to grow at high cell densities. Of the E. coli strains tested, including JM105, B, W3110, W3100, HB101, DH1, CSH50, MC1060, JRG1046, and JRG1061, strains JM105 and B were found to have the greatest relative biomass accumulation, strain MC1060 accumulated the highest concentrations of acetic acid, and strain B had the highest growth rates under the conditions tested. In glucose-feedback-controlled fed-batch fermentations, strains B and JM105 produced only 2 g of acetate.liter-1 while accumulating up to 30 g of biomass.liter-1. Under identical conditions, strains HB101 and MC1060 accumulated less than 10 g of biomass.liter-1 and strain MC1060 produced 8 g of acetate.liter-1. The addition of various concentrations of sodium acetate to the growth medium resulted in a logarithmic decrease, with respect to acetate concentration, in the growth rates of E. coli JM105, JM105(pOS4201), and JRG1061. These data indicated that the growth of the E. coli strains was likely to be inhibited by the acetate they produced when grown on media containing glucose. A model for the inhibition of growth of E. coli by acetate was derived from these experiments to explain the inhibition of acetate on E. coli strains at neutral pH.

[1]  T. Holme,et al.  Influence of glucose and dissolved oxygen concentrations on yields of Escherichia coli B in dialysis culture. , 1977, Journal of general microbiology.

[2]  D. Hentges,et al.  Shigella flexneri Inhibition by Acetic Acid , 1973, Infection and immunity.

[3]  J. Guest.  Anaerobic growth of Escherichia coli K12 with fumarate as terminal electron acceptor. Genetic studies with menaquinone and fluoroacetate-resistant mutants. , 1979, Journal of general microbiology.

[4]  Armin Fiechter,et al.  Mass culture of Escherichia coli: Medium development for low and high density cultivation of Escherichia coli B/r in minimal and complex media , 1985 .

[5]  H W Doelle,et al.  Effect of specific growth rate and glucose concentration on growth and glucose metabolism of Escherichia coli K-12. , 1976, Microbios.

[6]  K. Andersen,et al.  Are growth rates of Escherichia coli in batch cultures limited by respiration? , 1980, Journal of bacteriology.

[7]  B D Davis,et al.  Regulation of alpha-ketoglutarate dehydrogenase formation in Escherichia coli. , 1965, The Journal of biological chemistry.

[8]  H. Doelle,et al.  Regulation of glucose metabolism in bacterial systems , 1982 .

[9]  P. Gerhardt,et al.  Microfiltration Culture Process for Enhanced Production of rDNA Receptor Cells of Escherichia Coli , 1984, Bio/Technology.

[10]  H. W. Doelle,et al.  Effect of glucose concentration on a number of enzymes involved in the aerobic and anaerobic utilization of glucose in turbidostat-cultures of Escherichia coli. , 1974, Microbios.

[11]  D. Zabriskie,et al.  Factors influencing productivity of fermentations employing recombinant microorganisms , 1986 .

[12]  J. Adler,et al.  Change in intracellular pH of Escherichia coli mediates the chemotactic response to certain attractants and repellents , 1981, Journal of bacteriology.

[13]  J W Wimpenny,et al.  Regulation of metabolism in facultative bacteria. II. Effects of aerobiosis, anaerobiosis and nutrition on the formation of Krebs cycle enzymes in Escherichia coli. , 1966, Biochimica et biophysica acta.

[14]  Steven M. Schlasner,et al.  An automatic, on-line glucose analyzer for feed-back control of fed-batch growth of Escherichia coli , 1987 .

[15]  H. Kornberg,et al.  The enzymic interconversion of acetate and acetyl-coenzyme A in Escherichia coli. , 1977, Journal of general microbiology.

[16]  B. Bachmann,et al.  Derivations and genotypes of some mutant derivatives of Escherichia coli K12 , 1987 .

[17]  Shoichi Shimizu,et al.  HIGH DENSITY CULTIVATION OF BIOMASS IN FED-BATCH SYSTEM WITH DO-STAT , 1979 .

[18]  W. Holms,et al.  Control of carbon flux to acetate excretion during growth of Escherichia coli in batch and continuous cultures. , 1989, Journal of general microbiology.

[19]  E. Ziv,et al.  Dense growth of aerobic bacteria in a bench‐scale fermentor , 1976, Biotechnology and bioengineering.

[20]  W. Strohl,et al.  Microcomputer-control of fermentation processes , 1986 .

[21]  I. Gleiser,et al.  Growth of E. Coli W to high cell concentration by oxygen level linked control of carbon source concentration , 1981 .