Measurements of elastic constants between probe DNA strands and a target DNA linker

This paper reports the measurement of elastic constants between two DNA strands that are simultaneously hybridized by a third target-DNA linker. Two probe-DNA strands that are immobilized on fluorescent beads and a target-DNA linker formed a hybridized assembly through the Watson-Crick based pairing. Elastic constants of the resulting assembly were measured using a force calibrated dual optical trap. This study can be used to detect the existence of a target-DNA linker with a specified nucleotide sequence, indicating its potential use in DNA biosensors.

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