Growth rate of polypeptide chains as a function of the cell growth rate in a mutant of Escherichia coli 15.

Abstract The average growth rate of polypeptide chains in cells growing at various rates has been calculated. These calculations are based on measurements of the number of active ribosomes and of the amounts of protein in different media. Cultures having a reciprocal doubling time (μ) greater than one doubling per hour exhibit a chain growth rate of 20 to 21 amino acids per second independent of μ. However, as μ decreases to less than one doubling per hour the average chain growth rate decreases significantly.

[1]  R. Franklin,et al.  Polyribosomes of Escherichia coli. I. Effects of monovalent cations on the distribution of polysomes, ribosomes and ribosomal subunits. , 1969, Journal of molecular biology.

[2]  R. Schleif.  Control of production of ribosomal protein. , 1967, Journal of molecular biology.

[3]  Alexander Rich,et al.  Polyribosomes of Growing Bacteria , 1967, Science.

[4]  K. Burton.  A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. , 1956, The Biochemical journal.

[5]  O. Maaløe,et al.  Dependency on medium and temperature of cell size and chemical composition during balanced grown of Salmonella typhimurium. , 1958, Journal of general microbiology.

[6]  P. Hanawalt.  Involvement of Synthesis of RNA in Thymineless Death , 1963, Nature.

[7]  J. Janin,et al.  Measurement of the unstable RNA in exponentially growing cultures of Bacillus subtilis and Escherichia coli. , 1968, Journal of molecular biology.

[8]  C. Guthrie,et al.  Initiation of Protein Synthesis : a Critical Test of the 30S Subunit Model , 1968, Nature.

[9]  R Rosset,et al.  Ribonucleic acid composition of bacteria as a function of growth rate. , 1966, Journal of molecular biology.

[10]  D. Schlessinger,et al.  Polyribosome metabolism in Escherichia coli. I. Extraction of polyribosomes and ribosomal subunits from fragile, growing Escherichia coli. , 1966, Journal of molecular biology.

[11]  M. Lubin,et al.  ON THE ROLE OF INTRACELLULAR POTASSIUM IN PROTEIN SYNTHESIS. , 1964, Biochimica et biophysica acta.

[12]  G. Godson,et al.  Use of Brij lysis as a general method to prepare polyribosomes from Escherichia coli. , 1967, Biochimica et biophysica acta.

[13]  J. Forchhammer,et al.  Evidence for reduced breakdown of messenger RNA during blocked transcription or translation in Escherichia coli. , 1969, Journal of molecular biology.

[14]  C. Kurland,et al.  The distribution of soluble and ribosomal RNA as a function of growth rate , 1963 .

[15]  M. Meselson,et al.  Cyclic dissociation into stable subunits and re-formation of ribosomes during bacterial growth. , 1968, Journal of molecular biology.

[16]  J. Forchhammer,et al.  Regulation of messenger RNA synthesis in Escherichia coli. , 1968, Journal of molecular biology.

[17]  A. Kepes,et al.  Peptide chain initiation and growth in the induced synthesis of β-galactosidase , 1966 .

[18]  G. Stent,et al.  RNA chain growth rates in Escherichia coli. , 1969, Journal of molecular biology.

[19]  F. Neidhardt,et al.  Studies on the role of ribonucleic acid in the growth of bacteria. , 1960, Biochimica et biophysica acta.

[20]  H. Bremer,et al.  Rate of synthesis of messenger ribonucleic acid in Escherichia coli. , 1968, Journal of molecular biology.

[21]  Niels Ole Kjeldgaabd.  Regulation of Nucleic Acid and Protein Formation in Bacteria , 1967 .

[22]  C. Kurland.  Molecular characterization of ribonucleic acid from Escherichia coli ribosomes , 1960 .

[23]  G. Stent,et al.  Peptide chain growth of -galactosidase in Escherichia coli. , 1968, Journal of molecular biology.

[24]  N. Kjeldgaard,et al.  The kinetics of ribonucleic acid- and protein formation in Salmonella typhimurium during the transition between different states of balance growth. , 1961, Biochimica et biophysica acta.

[25]  R. Kohler,,et al.  Significance of the free 70 s ribosomes in Escherichia coli extracts. , 1968, Journal of molecular biology.

[26]  L. H. Stickland.  The determination of small quantities of bacteria by means of the biuret reaction. , 1951, Journal of general microbiology.

[27]  R. Harvey.  Regulation of Ribosomal Protein Synthesis in Escherichia coli , 1970, Journal of bacteriology.

[28]  H. Bremer,et al.  RNA chain growth-rate in Escherichia coli. , 1968, Journal of molecular biology.

[29]  M. Hoagland,et al.  Polyribosomes from Escherichia coli: Enzymatic Method for Isolation , 1965, Science.