DNA mediated sequential self-assembly of nano/micro components

A novel sequential self-assembly process of nano/micro components applying deoxyribonucleic acid (DNA) hybridization was proposed for the first time and its validity was experimentally verified. In the proposed process, characteristics of DNA, such as hybridization specificity of complementary pairs of single-stranded DNA (ssDNA) into a double-stranded DNA (dsDNA), and the dependence of hybridization on ambient temperature (melting temperature Tm) plays a key role. These characteristics of DNA can be designed and synthesized by base-pair sequence of DNA. Three types of self-assembly experiments using two types of ssDNA with different Tm (65degC and 50degC) were carried out; 1.) between Au nanoparticles, 2.) between Au nanoparticle and Au coated substrate, and 3.) between silicon micro components (5times5times3 mum3) and Au coated substrate. Through these experiments, it was successfully verified that the sequence of the self-assembly can be controlled by controlling the ambient temperature. The reversibility of this process was also confirmed. However, the silicon micro components assembly on Au coated substrate was not confirmed.

[1]  Chad A. Mirkin,et al.  DNA-Directed Synthesis of Binary Nanoparticle Network Materials , 1998 .

[2]  A. Steel,et al.  Electrochemical quantitation of DNA immobilized on gold. , 1998, Analytical chemistry.

[3]  H. McNallya,et al.  Self-assembly of micro-and nano-scale particles using bio-inspired events , 2003 .

[4]  R. Howe,et al.  Microstructure to substrate self-assembly using capillary forces , 2001 .

[5]  Simona Cocco,et al.  Force and kinetic barriers to unzipping of the DNA double helix , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[6]  Kenneth Y. Goldberg,et al.  Parallel microassembly with electrostatic force fields , 1998, Proceedings. 1998 IEEE International Conference on Robotics and Automation (Cat. No.98CH36146).

[7]  I. Shimoyama,et al.  Sequential self-assembly by controlling interactive forces between microparticles , 2004, 17th IEEE International Conference on Micro Electro Mechanical Systems. Maastricht MEMS 2004 Technical Digest.

[8]  C. Mirkin.  Programming the assembly of two- and three-dimensional architectures with DNA and nanoscale inorganic building blocks. , 2000, Inorganic chemistry.