Abstract This paper presents students’ feedback on the design and development of a Website for Biology, namely e-AV Biology for senior high schools in Indonesia. The teaching media was developed with the main feature of video lessons and other features supporting the learning of Biology such as the Interactive Quiz and Discussion to support students’ learning in Biology. Some video lessons described Renewable Energy on the field of Biotechnology Industrial, which is one of the topics that commonly difficult to visualize and explain. There is a need of aiding the explanation of Biodiesel sources, Biodiesel production process and Biodiesel usages by using audio visual. This study examined the design (Features and Interactivity, Video Content) and the Learning Impact of e-AV Biology Website. A total of 256 high school students participated in a larger study of a quasi- experiment in year 2011 with the intervention of two different ways of teaching, one with fully media instruction using e-AV Biology teaching method and another one the traditional manner of teaching. However, for the purpose of this paper, only the perception of the students participated in experiment group (n= 121, with 75 females, 46 males) were used, analyzed and reported. The last section of the paper presents the findings which indicated that majority students have positive response to overall feedback towards e-AV Biology teaching method, particularly the Features and Interactivity that provided in e-AV Biology Website.
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