Changes in glucose transporters in muscle in response to exercise.

The mechanism underlying the increase in glucose uptake in response to muscular contraction is not known, although it has been established that the change does not require insulin. It is our hypothesis that exercise, like insulin, stimulates translocation of glucose transporters to the plasma membrane. To test this hypothesis an experiment was performed to determine whether glucose transporters are translocated from an intracellular membrane to the plasma membrane during exercise. Untrained male rats weighing approximately 250 g were exercised by treadmill running for 2 h at 25 m/min. They were killed immediately after completion of exercise, and the gastrocnemius and quadriceps muscles were quickly removed. Sedentary animals were treated in the same way. Plasma and intracellular membranes were isolated by sucrose density gradient centrifugation and cytochalasin B binding assays were performed. Exercise resulted in a redistribution of glucose transporters from the intracellular membrane to the plasma membrane. The ratio of cytochalasin B binding sites in the membrane fractions (intracellular/plasma membrane) was 3.2 +/- 0.6 in rested animals and 1.3 +/- 0.3 after exercise. The concentration of glucose transporters was increased in the plasma membrane (from 19.8 +/- 1.8 to 30.4 +/- 3.9 pmol/mg protein) and decreased in the intracellular membrane (from 20.7 +/- 3.0 to 10.8 +/- 1.1 pmol/mg protein) in response to exercise. These results suggest that at least part of the increase in glucose uptake that occurs during exercise is the result of a redistribution of glucose transporters to the plasma membrane.

[1]  G. Dohm,et al.  An in vitro human muscle preparation suitable for metabolic studies. Decreased insulin stimulation of glucose transport in muscle from morbidly obese and diabetic subjects. , 1988, The Journal of clinical investigation.

[2]  F. Assimacopoulos-Jeannet,et al.  Effects of insulin on glucose transport and glucose transporters in rat heart. , 1988, The Biochemical journal.

[3]  B. Obermaier,et al.  Phorbol esters imitate in rat fat-cells the full effect of insulin on glucose-carrier translocation, but not on 3-O-methylglucose-transport activity. , 1988, The Biochemical journal.

[4]  A. Klip,et al.  Insulin‐induced translocation of glucose transporters in rat hindlimb muscles , 1987, FEBS letters.

[5]  R. Horuk,et al.  Dissociation of insulin-stimulated glucose transport from the translocation of glucose carriers in rat adipose cells. , 1987, The Journal of biological chemistry.

[6]  E. Richter,et al.  Increased Muscle Glucose Uptake After Exercise: No Need for Insulin During Exercise , 1985, Diabetes.

[7]  H. Wallberg-henriksson,et al.  Contractile activity increases glucose uptake by muscle in severely diabetic rats. , 1984, Journal of applied physiology: respiratory, environmental and exercise physiology.

[8]  E. Newsholme,et al.  A problem in the radiochemical assay of glucose-6-phosphatase in muscle. , 1984, The Biochemical journal.

[9]  E. Newsholme,et al.  Metabolic control of hepatic gluconeogenesis during exercise. , 1983, The Biochemical journal.

[10]  E. Newsholme,et al.  Sensitivity to insulin of glycolysis and glycogen synthesis of isolated soleus-muscle strips from sedentary, exercised and exercise-trained rats. , 1983, The Biochemical journal.

[11]  A. Klip,et al.  The glucose transport system of muscle plasma membranes: characterization by means of [3H]cytochalasin B binding. , 1983, Archives of biochemistry and biophysics.

[12]  N. Ruderman,et al.  Muscle glucose metabolism following exercise in the rat: increased sensitivity to insulin. , 1982, The Journal of clinical investigation.

[13]  G. Beecher,et al.  Effect of exercise on synthesis and degradation of muscle protein. , 1980, The Biochemical journal.

[14]  H. Mitsuda,et al.  Purification and enzymatic properties of gamma-glutamyltransferase from bovine colostrum. , 1978, Journal of biochemistry.

[15]  L. Jorfeldt,et al.  Glucose metabolism during leg exercise in man. , 1971, The Journal of clinical investigation.

[16]  N. Ruderman,et al.  Evaluation of the isolated perfused rat hindquarter for the study of muscle metabolism. , 1971, The Biochemical journal.

[17]  J. Holloszy,et al.  Studies of tissue permeability. X. Changes in permeability to 3-methylglucose associated with contraction of isolated frog muscle. , 1965, The Journal of biological chemistry.

[18]  T. J. Wheeler,et al.  The glucose transporter of mammalian cells. , 1985, Annual review of physiology.

[19]  A. Meister,et al.  Glutathione and related gamma-glutamyl compounds: biosynthesis and utilization. , 1976, Annual review of biochemistry.

[20]  N. Ruderman,et al.  Glucose metabolism in perfused skeletal muscle. Interaction of insulin and exercise on glucose uptake. , 1975, The Biochemical journal.