Reagentless detection of microorganisms by intrinsic fluorescence.

Quick and accurate detection of microbial contamination is accomplished by a unique combination of leading-edge technologies described in this and the accompanying paper. In this contribution, a hand-held prototype instrument is described which is capable of statistically sampling the environment for microbial contamination and determining cell viability. The technology is sensitive enough to detect very low levels ( approximately 20 cells/cm(2) or cm(3)) of microbes in seconds.

[1]  J. Ezzell,et al.  Current laboratory methods for biological threat agent identification. , 2001, Clinics in laboratory medicine.

[2]  J. Amesz,et al.  Fluorescence spectrophotometry of reduced phosphopyridine nucleotide in intact cells in the near-ultraviolet and visible region. , 1957, Biochimica et biophysica acta.

[3]  J. Lakowicz.  Topics in fluorescence spectroscopy , 2002 .

[4]  B Chance,et al.  Intracellular oxidation-reduction state measured in situ by a multichannel fiber-optic surface fluorometer. , 1982, Science.

[5]  C. Estes,et al.  Array based design of multi-wavelength fluorescence system , 2001, 2001 Conference Proceedings of the 23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society.

[6]  I Tothill,et al.  Microbial detection. , 1996, Biosensors & bioelectronics.

[7]  Yorgo Istefanopulos.  2001 conference proceedings of the 23rd annual International Conference of the IEEE Engineering in Medicine and Biology Society, 25-28 October, 2001, Istanbul, Turkey : building new bridges at the frontiers of engineering and medicibe , 2001 .

[8]  B. Chance,et al.  Ischemic areas in perfused rat hearts: measurement by NADH fluorescence photography. , 1976, Science.

[9]  Howard H. Weetall,et al.  Autoflurosecence detection of Escherichia coli on silver membrane filters , 1994 .

[10]  C. Estes,et al.  Algorithm for recognition of optical spectra in an environment containing interferants , 2001, 2001 Conference Proceedings of the 23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society.

[11]  A. Duncan,et al.  Low noise amplification for optical detector arrays [microbes detection] , 2001, 2001 Conference Proceedings of the 23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society.

[12]  B. Chance,et al.  Localization and kinetics of reduced pyridine nucleotide in living cells by microfluorometry. , 1959, The Journal of biological chemistry.

[13]  W. H. Nelson,et al.  The Steady-State and Decay Characteristics of Primary Fluorescence from Live Bacteria , 1987 .

[14]  C. Estes,et al.  Microbioengineering: microbe capture and detection , 2001, 2001 Conference Proceedings of the 23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society.

[15]  J H Luong,et al.  Applications of NADH-dependent fluorescence sensors for monitoring and controlling bioprocesses. , 1990, Bioprocess technology.

[16]  B. Chance,et al.  On the fluorescence of NAD(P)H in whole-cell preparations of tumours and normal tissues. , 1970, European journal of biochemistry.

[17]  K. Nealson,et al.  Bacterial bioluminescence: its control and ecological significance , 1979, Microbiological reviews.

[18]  I Gryczynski,et al.  Fluorescence of reduced nicotinamides using one- and two-photon excitation. , 1996, Biophysical chemistry.