Traffic-Related Air Pollution Affects Peak Expiratory Flow, Exhaled Nitric Oxide, and Inflammatory Nasal Markers

Abstract The authors used a longitudinal observational design, with repeated measures, to study the association between traffic-related air pollutants (i.e., nitric oxide, nitrogen dioxide, carbon monoxide, and Black Smoke) and respiratory symptoms. Subjects (N = 82) attended an elementary school in either Utrecht (i.e., urban children) or Bilthoven (i.e., suburban children). These two geographic areas differed with respect to levels of Black Smoke (means = 53 μg/m3 and 18 μg/m3, respectively). Levels of nitric oxide, nitrogen dioxide, carbon monoxide, and Black Smoke were consistently higher in Utrecht than in Bilthoven (mean daily ratios were 8,1.5, 1.8, and 2.7, respectively). The authors compared mean levels of short-term effects of the aforementioned air pollutants on suburban and urban children. Urban children had higher mean levels (p = .05) of interleukin-8 (32%), urea (39%), uric acid (26%), albumin (15%), and nitric oxide metabolites (21 %) in nasal lavage than did suburban children. Peak expiratory flow, exhaled nitric oxide levels, and nasal markers were associated with levels of particulate matter with diameters less than or equal to 10 urn, Black Smoke, nitrogen dioxide, and nitric oxide. With respect to per-unit increases in air pollution, urban children had more increased peak expiratory flow, higher levels of exhaled nitric oxide, and more increased release of uric acid, urea, and nitric oxide metabolites than suburban children. In summary, urban children had increased levels of inflammatory nasal markers, and their responses were more pronounced than were the suburban children's responses to the same increments of air pollution.

[1]  B. Brunekreef,et al.  Time trends in repeated spirometry in children. , 1992, The European respiratory journal.

[2]  B. Brunekreef,et al.  Asthma severity and susceptibility to air pollution. , 1998, The European respiratory journal.

[3]  D. Tsikas,et al.  Nitric oxide inhibits the secretion of T‐helper 1‐ and T‐helper 2‐associated cytokines in activated human T cells‘pa , 1997 .

[4]  H. Koren,et al.  Biomarkers of inflammation in ozone-exposed humans. Comparison of the nasal and bronchoalveolar lavage. , 1990, The American review of respiratory disease.

[5]  J. Frölich,et al.  Nitric oxide inhibits the secretion of T-helper 1- and T-helper 2-associated cytokines in activated human T cells. , 1997, Immunology.

[6]  R. Littell.  SAS System for Mixed Models , 1996 .

[7]  A. Zwinderman,et al.  Effects of photochemical air pollution and allergen exposure on upper respiratory tract inflammation in asthmatics. , 1997, American journal of respiratory and critical care medicine.

[8]  P. Barnes,et al.  Nitric oxide and airway disease. , 1995, Annals of medicine.

[9]  B. Brunekreef,et al.  Effects of low-level winter air pollution concentrations on respiratory health of Dutch children. , 1994, Environmental research.

[10]  L. van Bree,et al.  Biomarkers in nasal lavage as a tool for the assessment of health effects of photochemical air pollution. A feasibility study with volunteers. , 1995, Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie.

[11]  P. Steerenberg,et al.  Nasal lavage as tool for health effect assessment of photochemical air pollution , 1996, Human & experimental toxicology.

[12]  J. Balmes,et al.  Effects of nitric acid gas alone or in combination with ozone on healthy volunteers. , 1993, The American review of respiratory disease.

[13]  J. V. van Amsterdam,et al.  The effect of air pollution on exhaled nitric oxide of atopic and nonatopic subjects. , 1999, Nitric oxide : biology and chemistry.

[14]  W. Watson,et al.  Albumin standards and the measurement of serum albumin with bromcresol green. , 1997, Clinica chimica acta; international journal of clinical chemistry.

[15]  J. V. van Amsterdam,et al.  A simple method to sample exhaled NO not contaminated by ambient NO from children and adults in epidemiological studies. , 2000, Nitric oxide : biology and chemistry.

[16]  S. Al-Dabbagh,et al.  The estimation of nitrate and nitrite in saliva and urine. , 1983, Analytical biochemistry.

[17]  H. Nitta,et al.  Respiratory health associated with exposure to automobile exhaust. III. Results of a cross-sectional study in 1987, and repeated pulmonary function tests from 1987 to 1990. , 1999, Archives of environmental health.

[18]  M. Wjst,et al.  Road traffic and adverse effects on respiratory health in children. , 1993, BMJ.

[19]  J. Mckillop,et al.  Interleukin 8. , 1993, Autoimmunity.

[20]  M. Studnicka,et al.  Ambient ozone causes upper airways inflammation in children. , 1993, The American review of respiratory disease.

[21]  C. Leeder.  Methods for Cohort Studies of Chronic Airflow Limitation , 1984 .

[22]  Human nasal mucosal changes after exposure to urban pollution. , 1994 .

[23]  Bert Brunekreef,et al.  Air Pollution from Truck Traffic and Lung Function in Children Living near Motorways , 1997, Epidemiology.

[24]  J. Balmes,et al.  Ozone-induced airway inflammation in human subjects as determined by airway lavage and biopsy. , 1993, The American review of respiratory disease.

[25]  J. Just,et al.  Short-term effect of winter air pollution on respiratory health of asthmatic children in Paris. , 1998, The European respiratory journal.

[26]  M. Lai,et al.  Nitric oxide increased interleukin‐4 expression in T lymphocytes , 1997, Immunology.

[27]  F. Liew,et al.  Regulation of lymphocyte functions by nitric oxide. , 1995, Current opinion in immunology.

[28]  B. Brunekreef,et al.  Reproducibility of childhood respiratory symptom questions. , 1992, The European respiratory journal.

[29]  M. Kaliner,et al.  Nasal secretion of the ozone scavenger uric acid. , 1993, The American review of respiratory disease.

[30]  W. Karmaus,et al.  Effect of moderate NO2 air pollution on the lung function of children with asthmatic symptoms. , 1994, Environmental research.

[31]  Rebecca Bascom,et al.  Health effects of outdoor air pollution , 1996 .

[32]  J D Spengler,et al.  Respiratory health and PM10 pollution. A daily time series analysis. , 1991, The American review of respiratory disease.

[33]  B. Brunekreef,et al.  Effect of ambient winter air pollution on respiratory health of children with chronic respiratory symptoms. , 1993, The American review of respiratory disease.

[34]  J. Douwes,et al.  Work related acute and (sub-)chronic airways inflammation assessed by nasal lavage in compost workers. , 1997 .

[35]  R K Griffiths,et al.  Hospital admissions for asthma in preschool children: relationship to major roads in Birmingham, United Kingdom. , 1994, Archives of environmental health.

[36]  J Schwartz,et al.  Particulate air pollution and chronic respiratory disease. , 1993, Environmental research.

[37]  J. V. van Amsterdam,et al.  Increased exhaled nitric oxide on days with high outdoor air pollution is of endogenous origin. , 1999, The European respiratory journal.

[38]  L. Calderón-Garcidueñas,et al.  Human nasal mucosal changes after exposure to urban pollution. , 1994, Environmental health perspectives.

[39]  E Lebret,et al.  Chronic respiratory symptoms in children and adults living along streets with high traffic density. , 1996, Occupational and environmental medicine.