Effects of temperature, salinity and irradiance on the growth of the harmful red tide dinoflagellate Cochlodinium polykrikoides Margalef (Dinophyceae)

The effects of temperature, salinity and irradiance on the growth of the harmful red tide dinoflagellate Cochlodinium polykrikoides were examined in the laboratory. From 60 different combinations of temperature (10-30°C) and salinity (10-40) under saturated irradiance, C. polykrikoides exhibited its maximum specific growth rate of 0.41 day -1 at a combination of 25°C and salinity of 34. Optimum growth rates of >0.3 day -1 were observed at temperatures ranging from 21 to 26° C and at salinities from 30 to 36. The organism did not grow at temperatures ≤10°C and only grew at salinities >30 if the temperature was >15°C. It was able to grow in temperatures ranging from 15 to 30° C and at salinities from 20 to 36. These values closely resembled those observed for this species in situ. It appears as if C. polykrikoides is a stenohaline organism that prefers high salinities, indicative of offshore waters. Temperature had the greatest influence on the growth rate, followed by salinity, and then the interaction between temperature and salinity. The optimum irradiance for growth was >90 μmol m -2 s -1 . Photoinhibition did not occur at 230 μmol m -2 s -1 , which was the maximum irradiance used in this study.

[1]  K. Porter,et al.  The use of DAPI for identifying and counting aquatic microflora1 , 1980 .

[2]  Roger Brown.  Effect of temperature , 1996 .

[3]  S. Morton,et al.  PROVASOLI‐GUILLARD NATIONAL CENTER FOR CULTURE OF MARINE PHYTOPLANKTON 1997 LIST OF STRAINS , 1997 .

[4]  J. Stein.  Handbook of Phycological methods - Culture methods and Growth measurements , 1973 .

[5]  M. Ellegaard,et al.  TEMPERATURE AND SALINITY EFFECTS ON GROWTH OF A NON‐CHAIN‐FORMING STRAIN OF FYMNODINIUM CATENATUM (DINOOHYCEAE) ESTABLISHED FROM A CYST FROM RECENT SEDIMENTS IN THE SOUND (øRESUND), DENMARK 1 , 1993 .

[6]  M. Ellegaard.  Temperature and salinity effects on growth of a non-chain-forming strain of Gymnodinium catenatum (Dinophyceae) established from a cyst from recent sediments in the sound (Oresund) , 1993 .

[7]  Janet R. Stein-Taylor.  Culture methods and growth measurements , 1973 .

[8]  峰生 山口,et al.  有害赤潮鞭毛藻Gymnodinium nagasakienseの増殖におよぼす水温,塩分および光強度の影響 , 1989 .

[9]  H. G. Kim,et al.  Harmful algal blooms in korean coastal waters focused on three fish killing dinoflagellates , 1998 .

[10]  Young-Sang Suh,et al.  Relationship between Sea Surface Temperature derived from NOAA Satellites and Cochlodinium polykrikoides Red Tide occurrence in Korean Coastal Waters , 2000 .

[11]  P. Tett,et al.  Problems in Modelling the Photosynthesis-Light Relationship for Phytoplankton , 1981 .

[12]  Engel G. Vrieling,et al.  TOXIC PHYTOPLANKTON BLOOMS IN THE SEA , 1993 .

[13]  峰生 山口,et al.  有害赤潮ラフィド藻Chattonella antiquaとC. marinaの増殖速度に及ぼす水温,塩分および光強度の影響 , 1991 .

[14]  H. Kim.  Recent Harmful Algal Blooms and Mitigation Strategies in Korea , 1997 .

[15]  Ramón Margalef,et al.  Hidrografía y fitoplancton de un área marina de la costa meridional de Puerto Rico , 1961 .

[16]  Hyun-Jin Cho,et al.  Regeneration from hyaline cysts of Cochlodinium polykrikoides (Gymnodiniales, Dinophyceae), a red tide organism along the Korean coast , 2002 .

[17]  Woong-Seo Kim,et al.  Prologue and Epilogue to the First International Symposium on Phytoplankton Blooms , 1997 .

[18]  S. Oh,et al.  Effects of temperature, salinity and irradiance on the growth of the toxic dinoflagellate Gymnodinium catenatum (Dinophyceae) isolated from Hiroshima Bay, Japan , 2002 .

[19]  K. Nagasaki,et al.  Effects of temperature and salinity on the growth of the red tide flagellates Heterocapsa circularisquama (Dinophyceae) and Chattonella verruculosa (Raphidophyceae) , 1997 .

[20]  R. Guillard,et al.  Culture of Phytoplankton for Feeding Marine Invertebrates , 1975 .

[21]  B. Osborne,et al.  Light and Photosynthesis in Aquatic Ecosystems. , 1985 .