Building of hFIX transgenic mice by spermatogenic cells

Human FIX expression vector pCMVIX was packaged by Effectene™ reagent and injected into mice seminiferous tubules with glass pipettes. The expressional frame of pCMVIX was examined by PCR and Southern blotting among 41 progenies. There were 2 (4%) mice being integrated with hFIX gene into chromosomes. 4.6 ng/mL of hFIX protein was expressed in plasma of one mouse, which was tested by ELISA. We demonstrated that building of transgenic animals by spermatogonial stem cells is an efficient method. Meanwhile, it has also been proved to be an alternative choice for mammary gland bioreactor.

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