The origin of intrahepatic bile duct cells in the mouse.

The origin of the intrahepatic bile ducts in the embryonic mouse liver was investigated. At 12.5 and 13.5 gestation days in the C3H/Tw strain mouse, the liver parenchyma contains morphologically and histochemically homogeneous immature hepatocytes but not bile duct cells. When the liver fragments were cultured in the testis, immature hepatocytes differentiated into large hepatocytes for the most part and also into bile duct cells. In contrast, when the similar liver fragments were cultured under the skin of newborn mice, bile duct cells differentiated much earlier in all transplants than those cultured in the testis. These bile duct cells were considered to be the intrahepatic bile duct cells, since they did not form biliary glands but possessed a basal lamina and histochemical characteristics of intrahepatic bile duct cells of the normal liver. The origin of the endodermal epithelial cells in the mouse liver is discussed with special attention to the differentiation of the intrahepatic bile duct cells from the immature hepatocytes.

[1]  M. Kusano,et al.  Observations on the fine structure of long-survived isolated hepatocytes inoculated into rat spleen. , 1982, Gastroenterology.

[2]  N. Shiojiri.  Enzymo- and immunocytochemical analyses of the differentiation of liver cells in the prenatal mouse. , 1981, Journal of embryology and experimental morphology.

[3]  G. Michalopoulos,et al.  Morphologic and histochemical analysis of hepatocytes transplanted into syngeneic hosts. , 1980, The American journal of pathology.

[4]  B. Flaks,et al.  A TECHNIQUE FOR LIVER TRANSPLANTATION IN THE INBRED MOUSE: A FINE STRUCTURAL STUDY OF ALLOGRAFT REJECTION , 1976, Transplantation.

[5]  T. Onoé,et al.  Effect of stirring during fixation upon immunofluorescence. Results with distribution of albumin-producing cells in liver. , 1976, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[6]  S. Iijima,et al.  LIGHT AND ELECTRON MICROSCOPIC STUDIES ON THE DEVELOPMENT OF PERIPORTAL BILE DUCTS OF THE HUMAN EMBRYO , 1974, Acta pathologica japonica.

[7]  R. L. Wood.  An electron microscope study of developing bile canaliculi in the rat , , 1965, The Anatomical record.

[8]  M. Karnovsky,et al.  A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron-microscopy , 1965 .

[9]  J. W. Wilson,et al.  HISTOGENESIS OF THE LIVER ^ † , 1963, Annals of the New York Academy of Sciences.

[10]  A. D. Bois.  CHAPTER 1 – THE EMBRYONIC LIVER , 1963 .

[11]  John H. Luft,et al.  IMPROVEMENTS IN EPOXY RESIN EMBEDDING METHODS , 1961, The Journal of biophysical and biochemical cytology.

[12]  A. Fischel.  Die Entwicklung der Leber , 1929 .

[13]  W. Bloom.  The embryogenesis of human bile capillaries and ducts , 1926 .