论文信息 - Asymmetric nozzle structure for particles converging into a highly confined region

Asymmetric nozzle structure for particles converging into a highly confined region

Abstract Asymmetric nozzle inlet was suggested for particles to converge into a highly confined region. The physical properties from the nano-scaled volumetric difference at nozzle inlet were investigated using computational fluid dynamics and particle motion measurement with fluorescence beads and erythrocytes. The asymmetric nozzle structure made particles converge efficiently without clogging at nozzle inlet. It could be used as a micro/nanofluidic unit for cell based assay in a microchip such as chip-based flow cytometer.

[1] Dong-Chul Han,et al. Plastic microchip flow cytometer based on 2- and 3-dimensional hydrodynamic flow focusing , 2003 .

[2] H. Chuang,et al. Micro-flow measurement with a laser diode micro-particle image velocimetry , 2004 .

[3] C. G. Phillips,et al. On the asymmetry of bifurcations in the bronchial tree. , 1997, Respiration physiology.

[4] Steven W Graves,et al. Nozzle design parameters and their effects on rapid sample delivery in flow cytometry. , 2002, Cytometry.

[5] Daniel L. Feeback,et al. Microfabrication and test of a three-dimensional polymer hydro-focusing unit for flow cytometry applications , 2005 .

[6] H. Andersson,et al. Microfluidic devices for cellomics: a review , 2003 .

[7] S. Takayama,et al. Use of Air-Liquid Two-Phase Flow in Hydrophobic Microfluidic Channels for Disposable Flow Cytometers , 2002 .

[8] M Al-Rubeai,et al. Using the Microcyte Flow Cytometer To Monitor Cell Number, Viability, and Apoptosis in Mammalian Cell Culture , 2000, Biotechnology progress.

[9] Y. Fung,et al. Remodeling of the Bifurcation Asymmetry of Right Coronary Ventricular Branches in Hypertrophy , 2000, Annals of Biomedical Engineering.

[10] S. Quake,et al. A microfabricated fluorescence-activated cell sorter , 1999, Nature Biotechnology.