Utilisation of cod backbone by biochemical fractionation

The backbone fraction obtained from industrial processing of Atlantic cod (Gadus morhua) yields about 15% of the whole fish weight. Due to a high content of muscle and bone proteins, it is a valuable raw material for further processing. In the present work minced backbones were subjected to gentle hydrolysis by proteinases to solubilize muscle proteins before the pure bone fraction was heat extracted to recover gelatine and a solid residual fraction containing protein and calcium. Five enzyme preparations were compared: the bacterial enzymes Alcalase, Neutrase and Protamex and preparations of pig and cod trypsins. Best recoveries of protein hydrolysates were obtained with the bacterial enzymes, but these hydrolysates were more bitter than the hydrolysates obtained by tryptic digestion. The gelatine extracted had relatively low molecular weight and may be suitable for technical applications or as a neutraceutical. Apparently the solid residual fraction was mainly collagenous structures containing little calcium.

[1]  G. Lalasidis,et al.  Two new methods of debittering protein hydrolysates and a fraction of hydrolysates with exceptionally high content of essential amino acids , 1978 .

[2]  A. A. Leach.  Appendix—Notes on a modification of the Neuman & Logan method for the determination of the hydroxyproline , 1960 .

[3]  K. Sugiyama,et al.  Characteristics of sardine muscle hydrolysates prepared by various enzymic treatments. , 1991 .

[4]  A. Telser,et al.  Regulation of functionally related enzymes during alternative developmental programs. , 1971, Biochimica et biophysica acta.

[5]  J. Synowiecki,et al.  Chemical composition and nutritional value of processing discards of cod (Gadus morhua) , 1991 .

[6]  Se-kwon Kim,et al.  Improvement of functional properties of cod frame protein hydrolysates using ultrafiltration membranes , 1999 .

[7]  Takeshi Nagai,et al.  Isolation of collagen from fish waste material — skin, bone and fins , 2000 .

[8]  B. Rasco,et al.  Fish Protein Hydrolysates: Production, Biochemical, and Functional Properties , 2000, Critical reviews in food science and nutrition.

[9]  K. Hjelmeland.  Proteinase inhibitors in the muscle and serum of cod (Gadus morhua). Isolation and characterization , 1983 .

[10]  A. Raae,et al.  Purification and characterization of chymotrypsin, trypsin and elastase like proteinases from cod (Gadus morhua L.). , 1989, Comparative biochemistry and physiology. B, Comparative biochemistry.

[11]  J. Seifert,et al.  Oral administration of (14)C labeled gelatin hydrolysate leads to an accumulation of radioactivity in cartilage of mice (C57/BL). , 1999, The Journal of nutrition.

[12]  M. Gudmundsson,et al.  Gelatin from cod skins as affected by chemical treatments , 1997 .

[13]  Joe M. Regenstein,et al.  Physicochemical and Sensory Characteristics of Fish Gelatin , 2000 .

[14]  L. P. Witnauer,et al.  The electric birefringence buildup curve as applied to the determination of the dipole moment of soluble collagen. , 1971, Biochimica et biophysica acta.

[15]  E. Lied,et al.  Enzymatic hydrolysis of by‐products from the fish‐filleting industry; chemical characterisation and nutritional evaluation , 2000 .

[16]  Koen Dewettinck,et al.  Agglomeration Tendency during Top-Spray Fluidized Bed Coating with Gelatin and Starch Hydrolysate , 1999 .

[17]  J. A. Arnesen,et al.  Preparation and characterisation of gelatine from the skin of harp seal (Phoca groendlandica). , 2002, Bioresource technology.

[18]  J. J. Connell.  Advances in fish science and technology , 1980 .

[19]  G. Takerkart,et al.  Experimental study and modelling of gelatin production from bone powder: elaboration of an overall kinetic scheme for the acid process , 1997 .

[20]  C. Weaver.  Calcium in Food Fortification Strategies , 1998 .

[21]  H. Hultin,et al.  LIQUIFYING COD FISH FRAMES UNDER ACIDIC CONDITIONS WITH A FUNGAL ENZYME , 1994 .

[22]  I. Batista,et al.  PREPARATION AND CHARACTERIZATION OF PEPTONES OBTAINED BY A TWO-STEP ENZYMATIC HYDROLYSIS OF WHOLE FISH , 1989 .

[23]  J. Raa,et al.  Purification and characterization of pepsins from the arctic fish capelin (Mallotus villosus). , 1983, Comparative biochemistry and physiology. A, Comparative physiology.

[24]  A. Gildberg,et al.  22 – Enhancing returns from greater utilization , 2002 .