Effects of bovine prolactin gene polymorphism within exon 4 on milk related traits and genetic trends in Iranian Holstein bulls.

In this study, polymorphism of prolactingene was analyzed as a candidate generesponsible for variation and genetic trends in milk yield and composition traits. Genomic DNAs were extracted from 268 semen samples belonged to Iranian Holstein bulls. Genotyping for the prolactin gene using PCR- RFLP technique and RsaI restriction endonuclease showed a mutation in 294-bp fragment located in exon 4. The frequencies of A and G alleles were 0.069 and 0.931, respectively. The allelic substitution effect was significant for milk and protein yield (p < 0.05). The G allele was unfavorable for milk and protein yield. Genetic trends for all analyzed traits were significant (p < 0.01) and that was progressive for milk, fat and protein yield, but diminishing for fat and protein percent. The effects of prolactin SNP on genetic trends and the difference between genetic trends produced by A and G alleles were not significant for all studied traits.

[1]  N. Ghasemi,et al.  Associations between prolactin gene polymorphism and milk production in montebeliard cows , 2009 .

[2]  J. B. Chauhan,et al.  Genotyping of the polymorphism within exon 3 of prolactin gene in various dairy breeds by PCR RFLP (Brief report) , 2008 .

[3]  C. Joshi,et al.  Prolactin Gene Polymorphism and its Association With Economic Traits in Kankrej Cattle , 2008 .

[4]  L. Zwierzchowski,et al.  Effect of New SNP Within Bovine Prolactin Gene Enhancer Region on Expression in the Pituitary Gland , 2007, Biochemical Genetics.

[5]  M. Alipanah,et al.  Association of prolactin gene variants with milk production traits in Russian Red Pied cattle , 2007 .

[6]  E. Czerniawska-Piątkowska,et al.  Association of genetic variants of bovine prolactin with milk production traits of Black-and-White and Jersey cattle , 2005 .

[7]  O. Lazebny,et al.  Association of DNA polymorphisms of the growth hormone and prolactin genes with milk productivity in Yaroslavl and Black-and-White cattle , 2005, Russian Journal of Genetics.

[8]  S. Kamiński,et al.  Nucleotide sequence polymorphism within exon 4 of the bovine prolactin gene and its associations with milk performance traits. , 2005, Journal of applied genetics.

[9]  M. Kostyuchenko,et al.  Polymorphism of Bovine Prolactin Gene: Microsatellites, PCR–RFLP , 2001, Russian Journal of Genetics.

[10]  U. Kuhnlein,et al.  The identification of the kappa-casein genotype in Holstein dairy cattle using the polymerase chain reaction , 1990, Theoretical and Applied Genetics.

[11]  A. Dybus.  Associations between Leu/Val polymorphism of growth hormone gene and milk production traits in Black-and-White cattle , 2002 .

[12]  Jingbin Yan,et al.  [Molecular cloning and analysis of bovine prolactin full-long genomic as well as cDNA sequences]. , 2002, Yi chuan xue bao = Acta genetica Sinica.

[13]  F. Dulout,et al.  Genetic variability and population structure in loci related to milk production traits in native Argentine Creole and commercial Argentine Holstein cattle , 1999 .

[14]  P. Kelly,et al.  Prolactin (PRL) and its receptor: actions, signal transduction pathways and phenotypes observed in PRL receptor knockout mice. , 1998, Endocrine reviews.

[15]  M. Bauerová,et al.  Simultaneous analysis of bovine growth hormone and prolactin alleles by multiplex PCR and RFLP , 1998 .

[16]  N. Horseman,et al.  Defective mammopoiesis, but normal hematopoiesis, in mice with a targeted disruption of the prolactin gene , 1997, The EMBO journal.

[17]  F. Pirchner,et al.  Polymorphisms at growth‐hormone and prolactin loci in Indian cattle and buffalo , 1995 .

[18]  C. Leroux,et al.  Prolactin gene expression in ovine and caprine mammary gland. , 1994, Neuroendocrinology.

[19]  J. Beckmann,et al.  Mapping of bovine prolactin and rhodopsin genes in hybrid somatic cells. , 2009, Animal genetics.

[20]  S. Camper,et al.  Characterization of the bovine prolactin gene. , 1984, DNA.

[21]  F. Rottman,et al.  Nucleotide sequence of bovine prolactin messenger RNA. Evidence for sequence polymorphism. , 1982, The Journal of biological chemistry.

[22]  H. Grüneberg,et al.  Introduction to quantitative genetics , 1960 .