Lipase-catalysed transesterification of high oleic sunflower oil

The study of enzymatic transesterification of high oleic sunflower oil with butanol by immobilised Lipozyme® was realised in n-hexane and in a solvent-free system. A simplified model, based on the Ping Pong Bi Bi with alcohol competitive inhibition mechanism, was proposed to describe transesterification kinetics. The solvent-free reaction medium was characterised by higher initial velocities and a 6-times volumic productivity but at the thermodynamic equilibrium only about 60% of the potential oleic acid is converted into ester, against 95% in n-hexane. The resulting product is a mixture of ester, monoglyceride, diglyceride and triglyceride with lubricant and surfactant properties and its composition depends on the initial substrate ratio.

[1]  K. Poutanen,et al.  Applications of immobilized lipases to transesterification and esterification reactions in nonaqueous systems. , 1993, Enzyme and microbial technology.

[2]  D. Combes,et al.  The role of silica gel in lipase-catalyzed esterification reactions of high-polar substrates , 1997 .

[3]  R. Willemot,et al.  Kinetic study of esterification by immobilized lipase in n‐hexane , 1990, FEBS letters.

[4]  A. Klibanov,et al.  The effect of water on enzyme action in organic media. , 1988, The Journal of biological chemistry.

[5]  Alain Marty,et al.  Continuous enzymatic transesterification of high oleic sunflower oil in a packed bed reactor: influence of the glycerol production , 1999 .

[6]  F. Ergan,et al.  Lipase-catalyzed production of wax esters , 1991 .

[7]  A. Marty,et al.  Combining solvent engineering and thermodynamic modeling to enhance selectivity during monoglyceride synthesis by lipase-catalyzed esterification. , 2001, Enzyme and microbial technology.

[8]  C. Laane,et al.  Optimization of biocatalysis in organic media. , 1987 .

[9]  R. Purdy.  High oleic sunflower: Physical and chemical characteristics , 1986 .

[10]  J. Shaw,et al.  Lipase-catalysed ethanolysis and isopropanolysis of triglycerides with long-chain fatty acids , 1991 .

[11]  Johannes Tramper,et al.  Biocatalysis in Organic Media , 1987 .

[12]  J. Prausnitz,et al.  Kinetics of lipase-catalysed interesterification of triglycerides in cyclohexane , 1991 .

[13]  A. Marty,et al.  Kinetics of lipase‐catalyzed esterification in supercritical CO2 , 1992, Biotechnology and bioengineering.

[14]  A. Marty,et al.  Continuous operation of lipase-catalyzed reactions in nonaqueous solvents: Influence of the production of hydrophilic compounds. , 1997, Biotechnology and bioengineering.

[15]  C. Hill,et al.  Kinetic modeling of interesterification reactions catalyzed by immobilized lipase , 1994, Biotechnology and bioengineering.

[16]  D. Combes,et al.  Geranyl acetate synthesis by lipase-catalyzed transesterification in supercritical carbon dioxide , 1993 .

[17]  A. Padt,et al.  The effect of organic solvents on the equilibrium position of enzymatic acylglycerol synthesis , 1993, Biotechnology and bioengineering.

[18]  Alexander M. Klibanov,et al.  Enzyme-catalyzed processes in organic solvents. , 1985 .