Colorimetric detection of influenza A (H1N1) virus by a peptide-functionalized polydiacetylene (PEP-PDA) nanosensor

We developed a peptide-functionalized polydiacetylene (PEP-PDA) nanosensor for pandemic H1N1 virus (pH1N1) detection with the naked eye. A PDA nanosensor was fabricated by nano-precipitation and modified with PEP for the specific recognition of pH1N1. The PEP-PDA nanosensor showed unique chromatic properties involving a colour change from blue to red in the presence of pH1N1. We believe that this nanosensor can be applied for the development of a commercially available kit for pH1N1 detection.

[1]  Xiaohu Zhang,et al.  Electrochemical assay to detect influenza viruses and measure drug susceptibility. , 2015, Angewandte Chemie.

[2]  B. A. Pindzola,et al.  Biosensing with polydiacetylene materials: structures, optical properties and applications. , 2007, Chemical communications.

[3]  Kangwon Lee,et al.  Recent advances in fluorescent and colorimetric conjugated polymer-based biosensors. , 2010, The Analyst.

[4]  Jong-Man Kim,et al.  Hydrochromic conjugated polymers for human sweat pore mapping , 2014, Nature Communications.

[5]  J. Greve,et al.  Fast, ultrasensitive virus detection using a Young interferometer sensor. , 2007, Nano letters.

[6]  C. Chong,et al.  Evaluation of a rapid diagnostic test, NanoSign® Influenza A/B Antigen, for detection of the 2009 pandemic influenza A/H1N1 viruses , 2010, Virology Journal.

[7]  Guonan Chen,et al.  Highly selective colorimetric bacteria sensing based on protein-capped nanoparticles. , 2015, The Analyst.

[8]  Kimihito Ito,et al.  Predicting the Antigenic Structure of the Pandemic (H1N1) 2009 Influenza Virus Hemagglutinin , 2010, PloS one.

[9]  X. An,et al.  Multifunctional polydiacetylene-liposome with controlled release and fluorescence tracing , 2014 .

[10]  L. Brammer,et al.  Detecting 2009 pandemic influenza A (H1N1) virus infection: availability of diagnostic testing led to rapid pandemic response. , 2011, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[11]  D. Suarez,et al.  Review of Rapid Molecular Diagnostic Tools for Avian Influenza Virus , 2007, Avian diseases.

[12]  G. Jarori,et al.  Physical and chemical characterization of enolase immobilized polydiacetylene Langmuir-Blodgett film , 2006 .

[13]  M. Sukwattanasinitt,et al.  Roles of head group architecture and side chain length on colorimetric response of polydiacetylene vesicles to temperature, ethanol and pH. , 2011, Journal of colloid and interface science.

[14]  Homology modelling and insilico analysis of neuraminidase protein in H1N1 Influenza A virus , 2011 .

[15]  Jong-Man Kim,et al.  A Colorimetric Hydrocarbon Sensor Employing a Swelling‐Induced Mechanochromic Polydiacetylene , 2014 .

[16]  Eun-Kyung Lim,et al.  Simple, rapid detection of influenza A (H1N1) viruses using a highly sensitive peptide-based molecular beacon. , 2016, Chemical communications.

[17]  Nilda de Fátima Ferreira Soares,et al.  Behaviour of polydiacetylene vesicles under different conditions of temperature, pH and chemical components of milk. , 2012, Food chemistry.

[18]  Hiroki Inoue,et al.  Sialic acid-mimic peptides as hemagglutinin inhibitors for anti-influenza therapy. , 2010, Journal of medicinal chemistry.

[19]  Rakchart Traiphol,et al.  High color stability and reversible thermochromism of polydiacetylene/zinc oxide nanocomposite in various organic solvents and polymer matrices , 2015 .

[20]  Oktay Yarimaga,et al.  Polydiacetylenes: supramolecular smart materials with a structural hierarchy for sensing, imaging and display applications. , 2012, Chemical communications.

[21]  Oliver Seitz,et al.  Hairpin peptide beacon: dual-labeled PNA-peptide-hybrids for protein detection. , 2007, Journal of the American Chemical Society.

[22]  H. Park,et al.  Polydiacetylene (PDA)-based colorimetric detection of biotin-streptavidin interactions. , 2006, Biosensors & bioelectronics.

[23]  J. Peiris,et al.  Comparative analytical sensitivities of six rapid influenza A antigen detection test kits for detection of influenza A subtypes H1N1, H3N2 and H5N1. , 2007, Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology.

[24]  Deborah H. Charych,et al.  Color and Chromism of Polydiacetylene Vesicles , 1998 .

[25]  H. Park,et al.  A polydiacetylene microchip based on a biotin-streptavidin interaction for the diagnosis of pathogen infections. , 2008, Small.

[26]  M. Bergeron,et al.  Multiplex Real-Time PCR Assay for Detection of Influenza and Human Respiratory Syncytial Viruses , 2004, Journal of Clinical Microbiology.

[27]  G. Gao,et al.  Influenza neuraminidase operates via a nucleophilic mechanism and can be targeted by covalent inhibitors , 2013, Nature Communications.

[28]  Rakchart Traiphol,et al.  Controlling the reversible thermochromism of polydiacetylene/zinc oxide nanocomposites by varying alkyl chain length. , 2013, Journal of colloid and interface science.

[29]  Yun Kyung Jung,et al.  Specific Colorimetric Detection of Proteins Using Bidentate Aptamer‐Conjugated Polydiacetylene (PDA) Liposomes , 2010 .

[30]  Qin Tu,et al.  Polydiacetylene liposome-encapsulated alginate hydrogel beads for Pb2+ detection with enhanced sensitivity , 2015 .

[31]  D. Evans,et al.  Detection and identification of human influenza viruses by the polymerase chain reaction. , 1991, Journal of virological methods.

[32]  Jinsang Kim,et al.  Polydiacetylene liposome microarray toward influenza a virus detection: effect of target size on turn-on signaling. , 2013, Macromolecular rapid communications.

[33]  Eun-Kyung Lim,et al.  Dextran-coated magnetic nanoclusters as highly sensitive contrast agents for magnetic resonance imaging of inflammatory macrophages , 2011 .

[34]  Sang Jun Sim,et al.  A Direct, Multiplex Biosensor Platform for Pathogen Detection Based on Cross‐linked Polydiacetylene (PDA) Supramolecules , 2009 .

[35]  Subash C B Gopinath,et al.  An efficient RNA aptamer against human influenza B virus hemagglutinin. , 2006, Journal of biochemistry.

[36]  Kevin W Plaxco,et al.  Peptide beacons: a new design for polypeptide-based optical biosensors. , 2007, Bioconjugate chemistry.

[37]  Yuquan Wei,et al.  Time–temperature chromatic sensor based on polydiacetylene (PDA) vesicle and amphiphilic copolymer , 2010 .

[38]  Daniel E. Noyola,et al.  Comparison of a New Neuraminidase Detection Assay with an Enzyme Immunoassay, Immunofluorescence, and Culture for Rapid Detection of Influenza A and B Viruses in Nasal Wash Specimens , 2000, Journal of Clinical Microbiology.

[39]  K. Plaxco,et al.  Chimeric peptide beacons: a direct polypeptide analog of DNA molecular beacons. , 2007, Chemical communications.

[40]  Richard B. Thompson,et al.  Reversible thermochromism in photopolymerized phosphatidylcholine vesicles , 1986 .

[41]  Beyond molecular beacons: optical sensors based on the binding-induced folding of proteins and polypeptides. , 2009, Chemistry.

[42]  A scanometric antibody probe for facile and sensitive immunoassays. , 2015, Chemical communications.

[43]  Madhukar Pai,et al.  Accuracy of Rapid Influenza Diagnostic Tests , 2012, Annals of Internal Medicine.

[44]  Jong-Man Kim,et al.  Layer-by-layer deposition of polydiacetylene vesicles and linear poly(sulfonates) , 2006 .

[45]  Wei Wei,et al.  Colorimetric detection of influenza A virus using antibody-functionalized gold nanoparticles. , 2015, The Analyst.

[46]  M. Los,et al.  Antibody modified gold nanoparticles for fast and selective, colorimetric T7 bacteriophage detection. , 2014, Bioconjugate chemistry.

[47]  X. An,et al.  Thermal and photic stimuli-responsive polydiacetylene liposomes with reversible fluorescence. , 2013, Nanoscale.

[48]  Dae-Ki Kim,et al.  Tools to Detect Influenza Virus , 2013, Yonsei medical journal.

[49]  K. Plaxco,et al.  Engineering a signal transduction mechanism for protein-based biosensors. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[50]  M. Sukwattanasinitt,et al.  Electronic absorption spectroscopy probed side-chain movement in chromic transitions of polydiacetylene vesicles. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[51]  H. Park,et al.  Universal Colorimetric Detection of Nucleic Acids Based on Polydiacetylene (PDA) Liposomes , 2008 .

[52]  Rakchart Traiphol,et al.  Dual colorimetric response of polydiacetylene/zinc oxide nanocomposites to low and high pH. , 2014, Journal of colloid and interface science.