In vitro rooting of hybrid hazelnuts (Corylus avellana × Corylus americana) in a temporary immersion system

Commercial micropropagation of hybrid hazelnuts (Corylus avellana L. × C. americana Marshall) has been limited, owing to their poor rooting ability in vitro as well as ex vitro, leading to high mortality of plantlets transplanted in the greenhouse. The objective of this study was to develop an efficient and cost-effective protocol for rooting and plantlet acclimation of in vitro grown hazelnut shoots. Efficient in vitro rooting was accomplished in a rocker-based temporary immersion bioreactor system. The use of a temporary immersion system (TIS) in combination with the inert substrate Oasis® In Vitro Express (IVE) significantly improved the in vitro rooting efficiency (100%) compared with semi-solid medium (27%) after four weeks of culture. A higher density (36 explants/vessel) of shoot explants in the TIS was found to support a significantly greater shoot height, chlorophyll content, and longest root length, compared with the lowest density treatment (12 explants/vessel). Efficiency of rooting and the number of roots formed were similar for both the high and low density of explants in the culture vessels, and the resulting plantlets exhibited > 80% survival in the greenhouse. These results demonstrate the usefulness of rocker-based TIS for commercial micropropagation of hazelnuts and, potentially, other tree species.

[1]  P. Saxena,et al.  Improved in vitro rooting in liquid culture using a two piece scaffold system , 2019, Engineering in life sciences.

[2]  M. Manokari,et al.  In vitro propagation, in vitro flowering, ex vitro root regeneration and foliar micro-morphological analysis of Hedyotis biflora (Linn.) Lam , 2019, Vegetos.

[3]  M. Ellena,et al.  Advances in micropropagation of hazelnut (Corylus avellana L.) in Chile , 2018, Acta Horticulturae.

[4]  P. M. Pijut,et al.  Rapid in vitro shoot multiplication of the recalcitrant species Juglans nigra L. , 2018, In Vitro Cellular & Developmental Biology - Plant.

[5]  J. Adelberg.  Bioreactors and “smart vessels” for large-scale propagation , 2017 .

[6]  J. Adelberg,et al.  Phenolic foam rooting matrices allows faster transfer and more rapid growth of Echinacea plants in greenhouse , 2017, In Vitro Cellular & Developmental Biology - Plant.

[7]  M. Shekhawat,et al.  Comparative foliar micromorphological studies of in vitro and field transferred plants of Morinda citrifolia , 2017 .

[8]  A. Saleh,et al.  The effects of different strength of MS media in solid and liquid media on in vitro growth of Typhonium flagelliforme , 2017 .

[9]  Kevin Piunno,et al.  Application of 3D printing to prototype and develop novel plant tissue culture systems , 2017, Plant Methods.

[10]  G. Prabhuling,et al.  Optimization of explants density for tissue culture propagation of banana cv. 'GRANDE NAINE'. , 2017 .

[11]  R. E. Boullani,et al.  Effect of explants density and size on the in vitro proliferation and growth of separated shoots of globe artichoke ( Cynara cardunculus var . scolymus L . ) , 2017 .

[12]  Chiachung Chen.  Cost analysis of plant micropropagation of Phalaenopsis , 2016, Plant Cell, Tissue and Organ Culture (PCTOC).

[13]  J. Adelberg,et al.  A rooting matrix and tissue culture vessel system yielded larger, faster growingEcheveriaplants , 2016 .

[14]  P. Saxena,et al.  An efficient temporary immersion system for micropropagation of hybrid hazelnut , 2016 .

[15]  Z. Jamal,et al.  EFFECT OF INOCULATION DENSITY ON POTATO MICROPROPAGATION , 2016 .

[16]  M. C. Piñero,et al.  A regime of high CO2 concentration improves the acclimatization process and increases plant quality and survival , 2015, Plant Cell, Tissue and Organ Culture (PCTOC).

[17]  So-Young Park,et al.  Physiological and biochemical changes during acclimatization in a Doritaenopsis hybrid cultivated in different microenvironments in vitro , 2014 .

[18]  W. Bridges,et al.  Relationships between nutrients and plant density in liquid media during micropropagation and acclimatization of turmeric , 2013, In Vitro Cellular & Developmental Biology - Plant.

[19]  So-Young Park,et al.  Sugar metabolism, photosynthesis, and growth of in vitro plantlets of Doritaenopsis under controlled microenvironmental conditions , 2013, In Vitro Cellular & Developmental Biology - Plant.

[20]  A. Economou.  FROM MICROCUTTING ROOTING TO MICROPLANT ESTABLISHMENT: KEY POINTS TO CONSIDER FOR MAXIMUM SUCCESS IN WOODY PLANTS , 2013 .

[21]  Tewodros Tadesse,et al.  EFFICIENT USE OF TEMPORARY IMMERSION BIOREACTOR (TIB) ON PINEAPPLE (Ananas comosus L.) MULTIPLICATION AND ROOTING ABILITY , 2013 .

[22]  J. Kaur,et al.  A liquid culture system for shoot proliferation and analysis of pharmaceutically active constituents of Catharanthus roseus (L.) G. Don , 2011, Plant Cell, Tissue and Organ Culture (PCTOC).

[23]  Sheela Chandra,et al.  Acclimatization of tissue cultured plantlets: from laboratory to land , 2010, Biotechnology Letters.

[24]  C. Damiano,et al.  Improving micropropagation of hazelnut Italian cultivars through temporary immersion system. , 2009 .

[25]  Y. Desjardins,et al.  In vitro culture of plants: a stressful activity! , 2009 .

[26]  Candy Carranza Álvarez,et al.  Plant regeneration through direct shoot formation from leaf cultures and from protocorm-like bodies derived from callus of Encyclia mariae (Orchidaceae), a threatened Mexican orchid , 2009 .

[27]  P. Saxena,et al.  Thidiazuron-induced regeneration of Echinacea purpurea L.: Micropropagation in solid and liquid culture systems , 2006, Plant Cell Reports.

[28]  B. Hazarika.  Morpho-physiological disorders in in vitro culture of plants , 2006 .

[29]  C. Damiano,et al.  Micropropagation of Hazelnut (Corylus Avellana L.) , 2005 .

[30]  A. Hvoslef-Eide,et al.  Liquid culture systems for in vitro plant propagation , 2005 .

[31]  F. Blakeway,et al.  Use of the temporary immersion bioreactor system (RITA®) for production of commercial Eucalyptus clones in Mondi Forests (SA) , 2005, Plant Cell, Tissue and Organ Culture.

[32]  J. Adelberg.  Efficiency in thin-film liquid system for Hosta micropropagation , 2005, Plant Cell, Tissue and Organ Culture.

[33]  P. Read,et al.  Improved Rooting and Acclimatization of Micropropagated Hazelnut Shoots , 2004 .

[34]  V. A. Savangikar.  Role of low cost options in tissue culture , 2004 .

[35]  C. W. Fennell,et al.  The regulation of plant growth and development in liquid culture , 2004 .

[36]  S. Das,et al.  Enhanced rate of multiplication and rooting through the use of coir in aseptic liquid culture media , 2002, Plant Cell, Tissue and Organ Culture.

[37]  D. Haisel,et al.  Acclimatization of Micropropagated Plants to Ex Vitro Conditions , 1999, Biologia Plantarum.

[38]  C. Robacker,et al.  A programmable micropropagation apparatus using cycled liquid medium , 1991, Plant Cell, Tissue and Organ Culture.

[39]  A. Roberts,et al.  The preparation in vitro of chrysanthemum for transplantation to soil , 1990, Plant Cell, Tissue and Organ Culture.

[40]  A. Roberts,et al.  The preparation in vitro of chrysanthemum for transplantation to soil , 1990, Plant Cell, Tissue and Organ Culture.

[41]  P. S. Naik,et al.  Effect of inoculation density on potato micropropagation , 2004, Plant Cell, Tissue and Organ Culture.

[42]  Xiaoling Yu,et al.  A micropropagation system for hazelnuts (Corylus species) , 1995 .

[43]  D. Cornu,et al.  Root development of in vitro hybrid walnut microcuttings in a vermiculite-containing gelrite medium , 1992 .

[44]  N. Bassil,et al.  MICROPROPAGATION OF THE HAZELNUT, CORYLUS AVELLANA , 1990 .

[45]  M. A. Smith,et al.  Vessel type, closure, and explant orientation influence in vitro performance of five woody species , 1990 .

[46]  C. Pérez,et al.  Hazelnut (Corylus avellana L.) , 1989 .

[47]  M. Donkin,et al.  PHOTOSYNTHETIC ACTIVITY OF CAULIFLOWER MERISTEM CULTURES IN VITRO AND AT TRANSPLANTING INTO SOIL , 1987 .

[48]  K. Gebhardt.  Development of a sterile cultivation system for rooting of shoot tip cultures (red raspberries) in duroplast foam , 1985 .

[49]  J. Driver,et al.  In Vitro Propagation of Paradox Walnut Rootstock , 1984, HortScience.

[50]  Marianne Hanson,et al.  The International Atomic Energy Agency , 2021, Nature.

[51]  F. Steward,et al.  A Technique for the Controlled Growth of Excised Plant Tissue in Liquid Media Under Aseptic Conditions , 1949, Nature.