Quantitative study of granule and Purkinje cells in the cerebellar cortex of the rat

The numerical densities of granule and Purkinje cells in the cerebellar cortex of the rat were determined by stereological methods. The density of Purkinje cells in our fixed material was 1,018 ± 39 per mm2 (mean ± s.e.m.) of Purkinje cell layer and that of granule cells 1.92 ± 0.03 × 106 per μl of granular layer. The total area of Purkinje cell layer was 332 mm2 and the volume of granular layer was 48 μl. The rat cerebellum therefore contains 3.38 × 105 Purkinje cells and 9.2 × 107 granule cells, from which there are 274 granule cells for each Purkinje cell. The density of granule cells and the density of parallel fibers in the molecular layer observed in a companion study indicate that the average length of a parallel fiber is around 5 mm.

[1]  R. Harvey.  Determination of the lengths of nonisotropic linear features in micrographs , 1986, The Anatomical record.

[2]  C. R. Pool.  Hematoxylin-eosin staining of OsO4-fixed epon-embedded tissue; prestaining oxidation by acidified H2O2. , 1969, Stain technology.

[3]  E. Mugnaini.  The length of cerebellar parallel fibers in chicken and rhesus monkey , 1983, The Journal of comparative neurology.

[4]  D. Hillman,et al.  Vulnerability of cerebellar development in malnutrition—I. Quantitation of layer volume and neuron numbers , 1981, Neuroscience.

[5]  V. Friedrich,et al.  Density and relative number of granule and purkinje cells in cerebellar cortex of cat , 1980, Neuroscience.

[6]  J. Dobbing,et al.  A stereological analysis of the cerebellar granule and purkinje cells of 30‐day‐old and adult rats undernourished during early postnatal life , 1980, The Journal of comparative neurology.

[7]  M. A. Williams,et al.  Quantitative Methods in Biology , 1978 .

[8]  V. Braitenberg,et al.  Morphological observations on the cerebellar cortex , 1958, The Journal of comparative neurology.

[9]  Floyd E. Bloom,et al.  Senescent microstructural changes in rat cerebellum , 1984, Brain Research.

[10]  J. Lauder,et al.  Effects of early hypo- and hyperthyroidism on development of rat cerebellar cortex. IV. The parallel fibers , 1978, Brain Research.

[11]  W. Lange.  Regional Differences in the Cytoarchitecture of the Cerebellar Cortex , 1982 .

[12]  J. Szentágothai,et al.  Quantitative histological analysis of the cerebellar cortex in the cat. I. Number and arrangement in space of the Purkinje cells. , 1971, Brain research.

[13]  R. Harvey,et al.  Quantitative study of the Purkinje cell dendritic spines in the rat cerebellum , 1988, The Journal of comparative neurology.

[14]  R. Harvey,et al.  Number of parallel fiber synapses on an individual Purkinje cell in the cerebellum of the rat , 1988, The Journal of comparative neurology.

[15]  J. Clos,et al.  Effects of undernutrition on cell formation in the rat brain and specially on cellular composition of the cerebellum , 1977, Brain Research.

[16]  D. Armstrong,et al.  A quantitative study of the purkinje cells in the cerebellum of the albino rat , 1970, The Journal of comparative neurology.

[17]  E. Weibel.  Stereological Methods. Practical methods for biological morphometry , 1979 .

[18]  H. Heinsen,et al.  [Regional differences in the numerical density of Purkinje cells in cerebella of albino rats of two strains]. , 1983, Acta anatomica.

[19]  T. Inukai.  On the loss of Purkinje cells, with advancing age, from the cerebellar cortex of the albino rat , 1928 .

[20]  P. Moran.  Measuring the length of a curve , 1966 .