Polyethylenimine-carbon nanotube nanohybrids for siRNA-mediated gene silencing at cellular level.

Carbon nanotubes (CNTs) covalently modified with low molecular weight polyethylenimine (PEI) are able to bind and deliver siRNA to cells with higher efficacy than a reference lipidic carrier. The performances of the nanohybrid are rationalized by the combination of the cell penetration and endosomal escape properties of CNTs and PEI, respectively.

[1]  M. Prato,et al.  Antitumor activity and prolonged survival by carbon-nanotube-mediated therapeutic siRNA silencing in a human lung xenograft model. , 2009, Small.

[2]  Zhuang Liu,et al.  Functionalization of carbon nanotubes via cleavable disulfide bonds for efficient intracellular delivery of siRNA and potent gene silencing. , 2005, Journal of the American Chemical Society.

[3]  Joel A. Cohen,et al.  Enhanced cell penetration of acid-degradable particles functionalized with cell-penetrating peptides. , 2008, Bioconjugate chemistry.

[4]  H. Westerblad,et al.  Knockdown of TRPC3 with siRNA coupled to carbon nanotubes results in decreased insulin‐mediated glucose uptake in adult skeletal muscle cells , 2009, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[5]  Sung Wan Kim,et al.  Efficient siRNA Delivery with Non-viral Polymeric Vehicles , 2009, Pharmaceutical Research.

[6]  E. Weiss,et al.  Proton sponge trick for pH-sensitive disassembly of polyethylenimine-based siRNA delivery systems. , 2010, Bioconjugate chemistry.

[7]  K. E. Lundin,et al.  Formulation and delivery of splice-correction antisense oligonucleotides by amino acid modified polyethylenimine. , 2010, Molecular pharmaceutics.

[8]  Eric A. Grulke,et al.  Dispersion of Carbon Nanotubes in Liquids , 2003 .

[9]  A. Hirsch,et al.  Functionalization of carbon nanotubes enables non-covalent binding and intracellular delivery of small interfering RNA for efficient knock-down of genes. , 2008, Biochemical and biophysical research communications.

[10]  D. Scherman,et al.  Functionalization of single- and multi-walled carbon nanotubes with cationic amphiphiles for plasmid DNA complexation and transfection , 2009 .

[11]  G. Zuber,et al.  Towards synthetic viruses. , 2001, Advanced drug delivery reviews.

[12]  Maurizio Prato,et al.  Enhanced cellular internalization and gene silencing with a series of cationic dendron‐multiwalled carbon nanotube:siRNA complexes , 2010, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[13]  Emmanuel Flahaut,et al.  Higher dispersion efficacy of functionalized carbon nanotubes in chemical and biological environments. , 2010, ACS nano.

[14]  Kostas Kostarelos,et al.  Hybrid polymer-grafted multiwalled carbon nanotubes for in vitro gene delivery. , 2010, Small.

[15]  T. Tuschl,et al.  Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells , 2001, Nature.

[16]  Cationic oligonucleotide-peptide conjugates with aggregating properties enter efficiently into cells while maintaining hybridization properties and enzymatic recognition. , 2006, Journal of the American Chemical Society.

[17]  Hongjie Dai,et al.  siRNA delivery into human T cells and primary cells with carbon-nanotube transporters. , 2007, Angewandte Chemie.

[18]  Zhuang Liu,et al.  Carbon nanotubes as intracellular transporters for proteins and DNA: an investigation of the uptake mechanism and pathway. , 2006, Angewandte Chemie.

[19]  Maurizio Prato,et al.  Synthesis and characterization of a carbon nanotube-dendron series for efficient siRNA delivery. , 2009, Journal of the American Chemical Society.

[20]  E. Weiss,et al.  Suppression of cervical carcinoma cell growth by intracytoplasmic codelivery of anti-oncoprotein E6 antibody and small interfering RNA , 2007, Molecular Cancer Therapeutics.

[21]  Mark E. Davis.  The first targeted delivery of siRNA in humans via a self-assembling, cyclodextrin polymer-based nanoparticle: from concept to clinic. , 2009, Molecular pharmaceutics.

[22]  Shiroh Futaki,et al.  A pH-sensitive fusogenic peptide facilitates endosomal escape and greatly enhances the gene silencing of siRNA-containing nanoparticles in vitro and in vivo. , 2009, Journal of controlled release : official journal of the Controlled Release Society.

[23]  G. Zuber,et al.  Self‐Assembling Polyethylenimine Derivatives Mediate Efficient siRNA Delivery in Mammalian Cells , 2008, Chembiochem : a European journal of chemical biology.

[24]  I. Khalil,et al.  Uptake Pathways and Subsequent Intracellular Trafficking in Nonviral Gene Delivery , 2006, Pharmacological Reviews.

[25]  D. Scherman,et al.  Design and Evaluation of Histidine-Rich Amphipathic Peptides for siRNA Delivery , 2010, Pharmaceutical Research.

[26]  K. Leong,et al.  Polyethylenimine-grafted multiwalled carbon nanotubes for secure noncovalent immobilization and efficient delivery of DNA. , 2005, Angewandte Chemie.

[27]  G. Zuber,et al.  Synthetic viruslike particles for targeted gene delivery to alphavbeta3 integrin-presenting endothelial cells. , 2009, Molecular Pharmaceutics.

[28]  V. Basiuk,et al.  Direct Solvent-Free Amination of Closed-Cap Carbon Nanotubes: A Link to Fullerene Chemistry , 2004 .

[29]  Terry W. J. Steele,et al.  Fast degrading polyesters as siRNA nano-carriers for pulmonary gene therapy , 2008, Journal of Controlled Release.

[30]  D. Scherman,et al.  A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[31]  M. Prato,et al.  Functionalized carbon nanotubes for plasmid DNA gene delivery. , 2004, Angewandte Chemie.

[32]  O. Danos,et al.  Polyethylenimine‐mediated gene delivery: a mechanistic study , 2001, The journal of gene medicine.

[33]  M. Prato,et al.  Cancer therapy: Small 10/2009 , 2009 .

[34]  Kathryn A. Whitehead,et al.  Lipid-like materials for low-dose, in vivo gene silencing , 2010, Proceedings of the National Academy of Sciences.

[35]  Yu-cheng Tseng,et al.  Self-assembled lipid nanomedicines for siRNA tumor targeting. , 2009, Journal of biomedical nanotechnology.

[36]  Chao-Shun Yang,et al.  Nanotubes functionalized with lipids and natural amino acid dendrimers: a new strategy to create nanomaterials for delivering systemic RNAi. , 2010, Bioconjugate chemistry.