Novel resistances to four potyviruses in tuber-bearing potato species, and temperature-sensitive expression of hypersensitive resistance to potato virus Y

Summary. Novel potyvirus resistance specificities were found in eight tested wild potato species (clones): hypersensitive resistance (HR) to potato Y potyvirus (PVY) strain groups PVYO in Solanum megistacrolobum and S. polyadenium and PVYN in S. stoloniferum; HR to potato V potyvirus (PW) in S. maglia, S. polyadenium, S. stoloniferum, S. sparsipilum and S. sucrense, HR to potato A potyvirus (PVA) strain group 1 in S. sucrense, and extreme resistance (ER) to PVA in S. polyadenium. S. commersonii and S. stoloniferum expressed HR to tobacco etch potyvirus (TEV) which has not been reported previously in potato species. The studied clone of S. stoloniferum expressed HR to all potyviruses and potyvirus strains tested. The clone of S. stoloniferum (2n = 48; nuclear DNA content (2C) = 3.6 pg) and S. chacoense (2n = 24; 2C=1.9 pg) were crossed and one hybrid (2n = 36; 2C = 2.9 pg) was obtained. The hybrid expressed HR to all tested potyviruses except PVA, which indicated that HR to PVA was controlled by a gene which is different from the genes (or gene) controlling HR to PVYO, PVYN, PVV and TEV in S. stoloniferum. On the other hand, S. chacoense and the hybrid expressed ER to cucumber mosaic cucumovirus (CMV), whereas S. stoloniferum was susceptible to CMV. All tested wild species and the six tested potato cultivars (S. tuberosum subsp. tuberosum) expressed HR to PVV. Expression of HR following infection with PVYN induced systemic acquired resistance (SAR) in S. chacoense. HR to PVYN in S. sparsipilum and S. sucrense and to PVYO in potato cv. Pito was efficiently expressed at lower temperatures (16/18°C) indicated by the development of distinct necrotic lesions and/or vein necrosis in inoculated leaves, whereas the HR was rendered less effective at higher temperatures (19/24°C) which was indicated by the development of systemic infection with leaf-drop and mosaic symptoms.

[1]  T. Candresse,et al.  Frequent occurrence of recombinant potyvirus isolates. , 1996, The Journal of general virology.

[2]  J. Valkonen,et al.  Comparison of resistance to potyviruses within Solanaceae : infection of potatoes with tobacco etch potyvirus and peppers with potato A and Y potyviruses , 1996 .

[3]  M. Clark,et al.  Analyses of solanaceous species using repetitive genomic DNA sequences isolated from Solanum brevidens , 1996 .

[4]  J. Valkonen,et al.  Patterns and barriers of cell-to-cell movement and lack of systemic spread of tobacco etch potyvirus (TEV-GUS) in Solanum brevidens , 1996 .

[5]  J. Ryals,et al.  Signal transduction in systemic acquired resistance. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[6]  BJ Staskawicz,et al.  Molecular genetics of plant disease resistance , 1995, Science.

[7]  J. Valkonen,et al.  Resistance to cucumber mosaic virus in potato , 1995 .

[8]  J. Valkonen.  Nuclear DNA content of the Solanum spp. in the series Etuberosa as determined by laser flow cytometry , 1994 .

[9]  J. Valkonen,et al.  Transfer of resistance to potato leafroll virus, potato virus Y and potato virus X from Solarium brevidens to S. tuberosum through symmetric and designed asymmetric somatic hybridisation , 1994 .

[10]  M. Saarma,et al.  Cloning and sequencing of the 3'-terminal region of potato virus YN (Russian isolate) RNA genome. , 1990, Nucleic acids research.

[11]  Roger A. C. Jones.  Strain group specific and virus specific hypersensitive reactions to infection with potyviruses in potato cultivars. , 1990 .

[12]  J. Dodds,et al.  Effect of chemical and heat therapy on virus concentrations in in vitro potato plantlets. , 1990 .

[13]  R. Jones.  Problems associated with potyviruses in potato certification‐field inspection and serological testing , 1987 .

[14]  R. Coutts,et al.  Effect of temperature on potato virus X infection in potato cultivars carrying different combinations of hypersensitivity genes , 1986 .

[15]  E. A. Weststeijn.  Lesion growth and virus localization in leaves of Nicotiana tabacum cv. Xanthi nc. after inoculation with tobacco mosaic virus and incubation alternately at 22°C and 32°C , 1981 .

[16]  R. Cook,et al.  Proceedings of the Association of Applied Biologists , 1972 .

[17]  G. Cockerham.  Genetical studies on resistance to potato viruses X and Y , 1970, Heredity.

[18]  Hawkes Jg.  Some observations on South American potatoes. , 1947 .

[19]  H. Mckinney,et al.  Genotype and temperature in relation to symptoms caused in Nicotiana by the mosaic virus; studies on the necrosis and the mottling reactions in certain species of Nicotiana and in tobacco hybrids infected with tobaccomosaic virus. , 1945, The Journal of heredity.

[20]  G. Cockerham.  The reactions of potato varieties to viruses X, A, B and C , 1943 .

[21]  J. Valkonen,et al.  Resistance to potato virus A and potato virus Y in potato cultivars grown in Finland , 1996 .

[22]  J. Valkonen,et al.  Three Strain Groups of Potato A Potyvirus Based on Hypersensitive Responses in Potato, Serological Properties, and Coat Protein Sequences , 1995 .

[23]  J. Valkonen,et al.  Extreme resistance is epistatic to hypersensitive resistance to potato virus YO in a Solanum tuberosum subsp. andigena-derived potato genotype , 1994 .

[24]  T. Gavrilenko,et al.  Somatic hybridization between Lycopersicon esculentum and non-tuberous Solanum species of the Etuberosa series , 1992 .

[25]  M. L. Romancer,et al.  La maladie des nécroses annulaires superficielles des tubercules : une affection de la pomme de terre, due au virus Y , 1991 .

[26]  P. Roggero,et al.  Biochemical changes during the necrotic systemic infection of tobacco plants by potato virus Y, necrotic strain , 1988 .

[27]  C. Fribourg,et al.  Characterization of a new potyvirus from potato , 1984 .

[28]  R. Fraser,et al.  Effects of temperature on the Tm-1 gene for resistance to tobacco mosaic virus in tomato , 1982 .

[29]  Roger A. C. Jones.  Host Plant Reactions, Some Properties, and Serology of Wild Potato Mosaic Virus , 1979 .