Antigenotoxic Effects and Possible Mechanism of Red Yeast (Sporidiobolus pararoseus) on Aflatoxin B1-Induced Mutagenesis

Red yeast (Sporidiobolus pararoseus), obtained from glycerol waste in the biodiesel process, has been used as a mycotoxin sorbent in some agricultural products. This study focused on the antigenotoxic effects of red yeast on aflatoxin B1 (AFB1)-induced mutagenesis, using a Salmonella mutation assay and a rat liver micronucleus test. Red yeast was sequentially extracted to obtain hexane, acetone, hot water, and residue fractions. Carbohydrates were mainly found in hot water extract (HWE), while proteins were observed in the residue fraction. The amount of lycopene in hexane extract (HE) was higher than the amount of β-carotene in HE. All red yeast extracts were not mutagenic in the Salmonella typhimurium strains TA98 and TA100 under the presence and absence of metabolic activation. Among the extracts obtained from red yeast, HE presented the strongest antimutagenicity against AFB1-induced mutagenesis in both strains, but HWE did not show any antimutagenicity. The oral administration of red yeast, HE, and HWE for 28 days was further investigated in rats. These extracts did not induce micronucleated hepatocytes. Furthermore, they modulated the activities of some detoxifying enzymes but did not alter the activities of various cytochrome P450 isozymes. Notably, they significantly decreased hepatic micronucleus formation in AFB1-initiated rats. HE altered the activity of hepatic glutathione-S-transferase but did not affect its protein expression. Taken together, the antigenotoxicity of red yeast against AFB1-induced mutagenesis might be partly due to the modulation of some detoxifying enzymes in AFB1 metabolism. β-Carotene and lycopene might be promising antigenotoxic compounds in red yeast.

[1]  R. Wongpoomchai,et al.  Inhibitory effect of purple rice husk extract on AFB1-induced micronucleus formation in rat liver through modulation of xenobiotic metabolizing enzymes , 2019, BMC Complementary and Alternative Medicine.

[2]  S. Altun,et al.  The effect of lycopene on hepatotoxicity of aflatoxin B1 in rats , 2019, Archives of physiology and biochemistry.

[3]  M. Selim,et al.  Aflatoxin B1: A review on metabolism, toxicity, occurrence in food, occupational exposure, and detoxification methods. , 2019, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[4]  Y. Murakami,et al.  Anti-inflammatory Activity of β-Carotene, Lycopene and Tri-n-butylborane, a Scavenger of Reactive Oxygen Species. , 2018, In vivo.

[5]  Masanori Watanabe,et al.  Bioconversion of biodiesel-derived crude glycerol into lipids and carotenoids by an oleaginous red yeast Sporidiobolus pararoseus KM281507 in an airlift bioreactor. , 2018, Journal of bioscience and bioengineering.

[6]  Atchara Manowattana.  Optimization of Carotenoids Production by Red Yeast Sporobolomyces pararoseus TISTR5213 Using Waste Glycerol as the Sole Carbon Source , 2017 .

[7]  Yuliang Cheng,et al.  Torulene and torularhodin, protects human prostate stromal cells from hydrogen peroxide-induced oxidative stress damage through the regulation of Bcl-2/Bax mediated apoptosis , 2017, Free radical research.

[8]  Yuliang Cheng,et al.  Anti-cancer effects of torulene, isolated from Sporidiobolus pararoseus, on human prostate cancer LNCaP and PC-3 cells via a mitochondrial signal pathway and the down-regulation of AR expression , 2017 .

[9]  F. Berthiller,et al.  Impact of food processing and detoxification treatments on mycotoxin contamination , 2016, Mycotoxin Research.

[10]  A. Józkowicz,et al.  Role of Nrf2/HO-1 system in development, oxidative stress response and diseases: an evolutionarily conserved mechanism , 2016, Cellular and Molecular Life Sciences.

[11]  M. Cestari,et al.  Genotoxicity and Mutagenicity , 2016 .

[12]  W. Tapingkae,et al.  EFFECT OF DIETARY RED YEAST ( Sporidioboluspararoseus ) SUPPLEMENTATION ON SMALL INTESTINAL HISTOMORPHOMETRY OF LAYING HENS , 2016 .

[13]  H. Mukhtar,et al.  Resveratrol nanoformulation for cancer prevention and therapy , 2015, Annals of the New York Academy of Sciences.

[14]  K. Jarukamjorn,et al.  Purple rice bran extract attenuates the aflatoxin B1-induced initiation stage of hepatocarcinogenesis by alteration of xenobiotic metabolizing enzymes. , 2015, Asian Pacific journal of cancer prevention : APJCP.

[15]  H. Qian,et al.  Tentative Identification of Torulene Cis/trans Geometrical Isomers Isolated from Sporidiobolus pararoseus by High-Performance Liquid Chromatography-Diode Array Detection-Mass Spectrometry and Preparation by Column Chromatography , 2013, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.

[16]  M. Torres-Ramos,et al.  Multiple Molecular and Cellular Mechanisms of Action of Lycopene in Cancer Inhibition , 2013, Evidence-based complementary and alternative medicine : eCAM.

[17]  O. Kovalchuk,et al.  Micronuclei in genotoxicity assessment: from genetics to epigenetics and beyond , 2013, Front. Genet..

[18]  C. Chaiyasut,et al.  Antimutagenicity and anticlastogenicity of glutinous purple rice hull using in vitro and in vivo testing systems , 2013, Molecular & Cellular Toxicology.

[19]  Zhen Zhang,et al.  Aflatoxin B1-induced hepatocellular carcinoma in developing countries: Geographical distribution, mechanism of action and prevention , 2013, Oncology letters.

[20]  A. Allameh,et al.  The Significance of Glutathione Conjugation in Aflatoxin Metabolism , 2013 .

[21]  Q. Ma.  Role of nrf2 in oxidative stress and toxicity. , 2013, Annual review of pharmacology and toxicology.

[22]  I. Samanta,et al.  Assessment of yeast cell wall as replacements for antibiotic growth promoters in broiler diets: effects on performance, intestinal histo-morphology and humoral immune responses. , 2012, Journal of animal physiology and animal nutrition.

[23]  Puttinan Meepowpan,et al.  Genotoxicity and antigenotoxicity of the methanol extract of Cleistocalyx nervosum var. paniala seed using a Salmonella mutation assay and rat liver micronucleus tests , 2012, Molecular & Cellular Toxicology.

[24]  Takuji Tanaka,et al.  Cancer Chemoprevention by Caroteno , 2012, Molecules.

[25]  R. Krska,et al.  Yeast cell based feed additives: studies on aflatoxin B1 and zearalenone , 2012, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment.

[26]  D. Morgavi,et al.  Effectiveness of modified yeast cell wall extracts to reduce aflatoxin B1 absorption in dairy ewes. , 2011, Journal of dairy science.

[27]  C. Lan,et al.  Human Antimicrobial Peptide LL-37 Inhibits Adhesion of Candida albicans by Interacting with Yeast Cell-Wall Carbohydrates , 2011, PloS one.

[28]  R. Wongpoomchai,et al.  Effect of pinocembrin isolated from Boesenbergia pandurata on xenobiotic-metabolizing enzymes in rat liver. , 2011, Drug metabolism letters.

[29]  J. T. Macgregor,et al.  Micronucleus assays in rodent tissues other than bone marrow. , 2011, Mutagenesis.

[30]  R. Coulombe,et al.  Aflatoxin B1 in poultry: toxicology, metabolism and prevention. , 2010, Research in veterinary science.

[31]  V. Vetvicka,et al.  β-Glucans, History, and the Present: Immunomodulatory Aspects and Mechanisms of Action , 2008, Journal of immunotoxicology.

[32]  L. Jespersen,et al.  Saccharomyces cerevisiae and lactic acid bacteria as potential mycotoxin decontaminating agents , 2006 .

[33]  Bharti Odhav,et al.  Aflatoxin B1-induced toxicity in HepG2 cells inhibited by carotenoids: morphology, apoptosis and DNA damage , 2006, Biological chemistry.

[34]  S. Veeriah,et al.  Modulation of xenobiotic metabolising enzymes by anticarcinogens -- focus on glutathione S-transferases and their role as targets of dietary chemoprevention in colorectal carcinogenesis. , 2005, Mutation research.

[35]  Raffaella Corvi,et al.  3.7. Genotoxicity and Mutagenicity , 2005 .

[36]  S. Nishimura,et al.  Carbohydrate analysis by a phenol-sulfuric acid method in microplate format. , 2005, Analytical biochemistry.

[37]  J. François,et al.  Comprehensive conformational study of key interactions involved in zearalenone complexation with beta-D-glucans. , 2004, Biomacromolecules.

[38]  O. Sommerburg,et al.  Cytotoxic and genotoxic effects of β-carotene breakdown products on primary rat hepatocytes , 2003 .

[39]  E. Golovinsky,et al.  Natural and synthetic inhibitors of UDP-glucuronosyltransferase. , 2001, Pharmacology & therapeutics.

[40]  R. Edenharder,et al.  In vitro antimutagenic and in vivo anticlastogenic effects of carotenoids and solvent extracts from fruits and vegetables rich in carotenoids. , 1998, Mutation research.

[41]  T. Campbell,et al.  Effects of carotenoids on aflatoxin B1-induced mutagenesis in S. typhimurium TA 100 and TA 98. , 1990, Nutrition and cancer.