A Comparative Study on Biological Removal of Cr(VI) by Fusarium Solani under Different Modes of Operational Strategies

In the present work biological removal of Cr(VI) was carried out in fed batch mode of operation using the growing cells of Fusarium solani isolated from soil. The fed batch process was studied by constant volume pulse feeding (CVPF) and increasing volume pulse feeding (IVPF), and the effects of these operational strategies on biological performance were compared with a conventional batch process. Batch studies indicated the maximum specific Cr(VI) removal to be 71 mg/g at pH 5.0 and at 500 mg/l initial Cr(VI) ion concentration. In CVPF process the maximum specific Cr(VI) removal was found to be 35.51 mg/g and 25.15 mg/g as compared to 51.9 mg/g and 42.5 mg/g obtained in IVPF during first and second pulse feedings respectively in both the cases. These results were compared with the Cr(VI) removal obtained in earlier studies conducted by the present authors using the continuous mode of operation. The continuous mode of operation was found to be the best operational strategy in which the process could be operated for longer duration with maximum specific Cr(VI) removal of 62.27 mg/g. Nearly complete removal was obtained using single stage reactor at lower Cr(VI) concentrations up to 100 mg/l and a multi stage reactor at higher Cr(VI) concentration.

[1]  Carlos Cervantes,et al.  Hexavalent-chromium reduction by a chromate-resistantBacillus sp. strain , 1995, Antonie van Leeuwenhoek.

[2]  N. Brown,et al.  Chromate reduction by Microbacterium liquefaciens immobilised in polyvinyl alcohol , 2004, Biotechnology Letters.

[3]  F. Congregado,et al.  Effect of culture medium ions on chromate reduction by resting cells of Agrobacterium radiobacter , 1993, Applied Microbiology and Biotechnology.

[4]  Z. Aksu,et al.  Bioaccumulation of copper(II), lead(II) and chromium(VI) by growing Aspergillus niger , 2003 .

[5]  H. Ohtake,et al.  Factors affecting chromate reduction in Enterobacter cloacae strain HO1 , 1989, Applied Microbiology and Biotechnology.

[6]  M. G. Dastidar,et al.  Hexavalent Chromium Reduction and Its Distribution in the Cell and Medium by Chromium Resistant Fusarium solani , 2013 .

[7]  M. G. Dastidar,et al.  Biosorption of Chromium (VI) by Nonliving Fusarium sp. Isolated from Soil , 2005 .

[8]  Mason R. Mackey,et al.  Cometabolism of Cr(VI) by Shewanella oneidensis MR‐1 produces cell‐associated reduced chromium and inhibits growth , 2003, Biotechnology and bioengineering.

[9]  O. Muter,et al.  Interrelations of the yeast Candida utilis and Cr(VI): metal reduction and its distribution in the cell and medium , 2001 .

[10]  B. Barkhordar,et al.  COMPARISION OF LANGMUIR AND FREUNDLICH EQUILIBRIUMS IN CR, CU AND NI ADSORPTION BY SARGASSUM , 2004 .

[11]  F. Esparza-García,et al.  Heavy metals removal by the microalga Scenedesmus incrassatulus in continuous cultures. , 2004, Bioresource technology.

[12]  G. L. Miller.  Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar , 1959 .

[13]  P. Ramteke,et al.  Chromium (VI) biosorption and bioaccumulation by chromate resistant bacteria. , 2002, Chemosphere.

[14]  M. Sen.  A Comparative Study on Biosorption of Cr(VI) by Fusarium solani under Different Growth Conditions , 2012 .

[15]  T. E. Abraham,et al.  Biosorption of Cr (VI) from aqueous solution by Rhizopus nigricans. , 2001, Bioresource technology.

[16]  S. Pattabhi,et al.  Removal of hexavalent chromium using distillery sludge. , 2003, Bioresource technology.

[17]  Z. Aksu,et al.  Competitive biosorption of phenol and chromium(VI) from binary mixtures onto dried anaerobic activated sludge , 2001 .

[18]  M. G. Dastidar,et al.  BIOSORPTION OF CR (VI) BY RESTING CELLS OF FUSARIUM SOLANI , 2011 .

[19]  M. G. Dastidar,et al.  BIOSORPTION OF CR (VI) BY RESTING CELLS OF ASPERGILLUS SP. , 2007 .

[20]  M. G. Dastidar,et al.  Biological removal of Cr(VI) using Fusarium solani in batch and continuous modes of operation , 2007 .

[21]  S. Hasnain,et al.  Microbial conversion of Cr (VI) in to Cr (III) in industrial effluent , 2004 .