290 and 340 nm UV LED arrays for fluorescence detection from single airborne particles.

We demonstrate a compact system, incorporating a 32-element linear array of ultraviolet (290 nm and 340 nm) light-emitting diodes (LEDs) and a multi-anode photomultiplier tube, to the in-flight fluorescence detection of aerosolized particles, here containing the biological molecules tryptophan and NADH. This system illustrates substantial advances in the growth and fabrication of new semiconductor UV light emitting devices and an evolution in packaging details for LEDs tailored to the bio-aerosol warning problem. Optical engineering strategies are employed which take advantage of the size and versatility of light-emitting diodes to develop a truly compact fluorescence detector.

[1]  Yong-Le Pan,et al.  Single-Particle Fluorescence Spectrometer for Ambient Aerosols , 2003 .

[2]  B V Bronk,et al.  Fluorescence from airborne microparticles: dependence on size, concentration of fluorophores, and illumination intensity. , 2001, Applied optics.

[3]  Grigory Simin,et al.  High-power deep ultraviolet light-emitting diodes basedon a micro-pixel design , 2004 .

[4]  Yong-Le Pan,et al.  High-speed, high-sensitivity aerosol fluorescence spectrum detection using a 32-anode photomultiplier tube detector , 2001 .

[5]  Yong-Le Pan,et al.  A Puff of Air Sorts Bioaerosols for Pathogen Identification , 2004 .

[6]  J. Ho,et al.  Design of an instrument for real-time detection of bioaerosols using simultaneous measurement of particle aerodynamic size and intrinsic fluorescence. , 1997, Journal of aerosol science.

[7]  E Hirst,et al.  Single particle multichannel bio-aerosol fluorescence sensor. , 2005, Optics express.

[8]  Yong-Le Pan,et al.  Real-time measurement of fluorescence spectra from single airborne biological particles , 1999 .

[9]  Vasanthi Sivaprakasam,et al.  Multiple UV wavelength excitation and fluorescence of bioaerosols , 2004, SPIE Optics East.

[10]  P. Hairston,et al.  Differences in Detected Fluorescence Among Several Bacterial Species Measured with a Direct-Reading Particle Sizer and Fluorescence Detector , 2000 .

[11]  Yong-Le Pan,et al.  Application of light-emitting diodes for aerosol fluorescence detection. , 2003, Optics letters.

[12]  Yoon-Kyu Song,et al.  High Performance AlGaInN Ultraviolet Light-Emitting Diode at the 340 nm Wavelength , 2004 .