Identification methods in microplastic analysis: a review

Microplastics have become a major global environmental issue in recent decades due to their ubiquity in the oceans, bioavailability and ability to carry toxic chemicals. Analysis of microplastics in environmental and experimental samples has become increasingly common. We conducted a literature survey to review the current identification methods for microplastics. The advantages and limitations of each identification method were compared to indicate the best methods for microplastic analysis. Identification of microplastics is based on the physical and chemical characterisation of isolated particles in mixtures of inorganic and organic remnant particles after the extraction and clean-up steps. In addition, novel methods which may possibly be applied to microplastic identification were suggested. Microscopic identification by itself has a high risk of producing both false positive and false negative results in analyses of small microplastics. At present, combinations of physical (e.g., microscopy) and chemical (e.g., spectroscopy) analyses are widely used. However, there is a need to improve and develop new methods to reduce the identification time and effort and to detect sub-micron plastics in environmental samples.

[1]  H. Saitoh,et al.  Characterization of microstructure of polyethylenes by differential scanning calorimetry , 1997 .

[2]  Kyun-Woo Lee,et al.  Size-dependent effects of micro polystyrene particles in the marine copepod Tigriopus japonicus. , 2013, Environmental science & technology.

[3]  Colin R. Janssen,et al.  Microplastic pollution in deep-sea sediments. , 2013, Environmental pollution.

[4]  Anthony L Andrady,et al.  Microplastics in the marine environment. , 2011, Marine pollution bulletin.

[5]  D. Fabbri.  Use of pyrolysis-gas chromatography/mass spectrometry to study environmental pollution caused by synthetic polymers: a case study: the Ravenna Lagoon , 2001 .

[6]  S. Moore,et al.  Plastic ingestion by planktivorous fishes in the North Pacific Central Gyre. , 2010, Marine pollution bulletin.

[7]  J. A. van Franeker,et al.  Monitoring plastic ingestion by the northern fulmar Fulmarus glacialis in the North Sea. , 2011, Environmental pollution.

[8]  J. Obbard,et al.  Prevalence of microplastics in Singapore's coastal marine environment. , 2006, Marine pollution bulletin.

[9]  N. V. von Moos,et al.  Uptake and effects of microplastics on cells and tissue of the blue mussel Mytilus edulis L. after an experimental exposure. , 2012, Environmental science & technology.

[10]  W. Shim,et al.  Distribution of small plastic debris in cross-section and high strandline on Heungnam beach, South Korea , 2013, Ocean Science Journal.

[11]  J Robbens,et al.  Quality assessment of the blue mussel (Mytilus edulis): comparison between commercial and wild types. , 2014, Marine pollution bulletin.

[12]  W. Shim,et al.  Potential Threat of Microplastics to Zooplanktivores in the Surface Waters of the Southern Sea of Korea , 2015, Archives of Environmental Contamination and Toxicology.

[13]  Melanie Sapp,et al.  Identification and Quantification of Microplastics in Wastewater Using Focal Plane Array-Based Reflectance Micro-FT-IR Imaging. , 2015, Analytical chemistry.

[14]  Jörg Klasmeier,et al.  Occurrence and spatial distribution of microplastics in sediments from Norderney. , 2014, Environmental pollution.

[15]  Dominique Remy,et al.  A new analytical approach for monitoring microplastics in marine sediments. , 2014, Environmental pollution.

[16]  Gunnar Gerdts,et al.  Methodology Used for the Detection and Identification of Microplastics—A Critical Appraisal , 2015 .

[17]  Colin R. Janssen,et al.  Microplastics in bivalves cultured for human consumption. , 2014, Environmental pollution.

[18]  Young Kyoung Song,et al.  Identification and quantification of microplastics using Nile Red staining. , 2016, Marine pollution bulletin.

[19]  S. Fowler,et al.  Spectrofluorometric studies of the lipid probe, nile red. , 1985, Journal of lipid research.

[20]  Emily E. Peacock,et al.  Plastic Accumulation in the North Atlantic Subtropical Gyre , 2010, Science.

[21]  M. Gregory,et al.  Plastic pellets on New Zealand beaches , 1977 .

[22]  Jundong Wang,et al.  Extraction, enumeration and identification methods for monitoring microplastics in the environment , 2016 .

[23]  Peter S Ross,et al.  Widespread distribution of microplastics in subsurface seawater in the NE Pacific Ocean. , 2014, Marine pollution bulletin.

[24]  Young Kyoung Song,et al.  Occurrence and Distribution of Microplastics in the Sea Surface Microlayer in Jinhae Bay, South Korea , 2015, Archives of Environmental Contamination and Toxicology.

[25]  Richard C. Thompson,et al.  Global warming releases microplastic legacy frozen in Arctic Sea ice , 2014 .

[26]  S. Fowler,et al.  Nile red: a selective fluorescent stain for intracellular lipid droplets , 1985, The Journal of cell biology.

[27]  Rowshyra A. Castañeda,et al.  Microplastic pollution in St. Lawrence River sediments , 2014 .

[28]  Julia Reisser,et al.  Plastic Pollution in the World's Oceans: More than 5 Trillion Plastic Pieces Weighing over 250,000 Tons Afloat at Sea , 2014, PloS one.

[29]  K. Das,et al.  Detection of Anthropogenic Particles in Fish Stomachs: An Isolation Method Adapted to Identification by Raman Spectroscopy , 2015, Archives of Environmental Contamination and Toxicology.

[30]  Curtis Marcott,et al.  AFM–IR: Combining Atomic Force Microscopy and Infrared Spectroscopy for Nanoscale Chemical Characterization , 2012, Applied spectroscopy.

[31]  Young Kyoung Song,et al.  Marine neustonic microplastics around the southeastern coast of Korea. , 2015, Marine pollution bulletin.

[32]  M. Thiel,et al.  Plastic pollution in the South Pacific subtropical gyre. , 2013, Marine pollution bulletin.

[33]  W. Shim,et al.  Microplastics in the Ocean , 2015, Archives of Environmental Contamination and Toxicology.

[34]  Martin Ebert,et al.  Identification of polymer types and additives in marine microplastic particles using pyrolysis-GC/MS and scanning electron microscopy. , 2013, Environmental science. Processes & impacts.

[35]  Fiona Murray,et al.  Plastic contamination in the decapod crustacean Nephrops norvegicus (Linnaeus, 1758). , 2011, Marine pollution bulletin.

[36]  R. Price,et al.  Extrinsic lactose fines improve dry powder inhaler formulation performance of a cohesive batch of budesonide via agglomerate formation and consequential co-deposition. , 2015, International journal of pharmaceutics.

[37]  D. Fabbri,et al.  Analysis of poly(vinyl chloride) and other polymers in sediments and suspended matter of a coastal lagoon by pyrolysis-gas chromatography-mass spectrometry , 2000 .

[38]  K. Dawson,et al.  Evidence for immunomodulation and apoptotic processes induced by cationic polystyrene nanoparticles in the hemocytes of the marine bivalve Mytilus. , 2015, Marine environmental research.

[39]  E. Carpenter,et al.  Polystyrene Spherules in Coastal Waters , 1972, Science.

[40]  A. Dazzi,et al.  Resonance enhanced AFM-IR: a new powerful way to characterize blooming on polymers used in medical devices. , 2015, International journal of pharmaceutics.

[41]  Young Kyoung Song,et al.  Large accumulation of micro-sized synthetic polymer particles in the sea surface microlayer. , 2014, Environmental science & technology.

[42]  P. Froneman,et al.  A quantitative analysis of microplastic pollution along the south-eastern coastline of South Africa. , 2015, Marine pollution bulletin.

[43]  M. Camaiti,et al.  Microplastic debris in sandhoppers , 2013 .

[44]  David A Cooper,et al.  Effects of mechanical and chemical processes on the degradation of plastic beach debris on the island of Kauai, Hawaii. , 2010, Marine pollution bulletin.

[45]  Julian Moger,et al.  Microplastic ingestion by zooplankton. , 2013, Environmental science & technology.

[46]  Richard C. Thompson,et al.  Microplastics in the marine environment: a review of the methods used for identification and quantification. , 2012, Environmental science & technology.

[47]  Young Kyoung Song,et al.  A comparison of microscopic and spectroscopic identification methods for analysis of microplastics in environmental samples. , 2015, Marine pollution bulletin.

[48]  Karla J McDermid,et al.  Quantitative analysis of small-plastic debris on beaches in the Hawaiian Archipelago. , 2004, Marine pollution bulletin.

[49]  Marius Majewsky,et al.  Determination of microplastic polyethylene (PE) and polypropylene (PP) in environmental samples using thermal analysis (TGA-DSC). , 2016, The Science of the total environment.

[50]  Martin Thiel,et al.  Distribution and abundance of small plastic debris on beaches in the SE Pacific (Chile): a study supported by a citizen science project. , 2013, Marine environmental research.

[51]  Richard C. Thompson,et al.  Spatial patterns of plastic debris along Estuarine shorelines. , 2010, Environmental science & technology.

[52]  M. Grego,et al.  Macrodebris and microplastics from beaches in Slovenia. , 2014, Marine pollution bulletin.

[53]  V. Moschino,et al.  Microplastic particles in sediments of Lagoon of Venice, Italy: First observations on occurrence, spatial patterns and identification , 2013 .

[54]  Alysse Mathalon,et al.  Microplastic fibers in the intertidal ecosystem surrounding Halifax Harbor, Nova Scotia. , 2014, Marine pollution bulletin.

[55]  Minyung Lee,et al.  Excited state dynamics of Nile Red in polymers , 2009 .

[56]  M. Jekel,et al.  Analysis of polyethylene microplastics in environmental samples, using a thermal decomposition method. , 2015, Water research.

[57]  M. Thiel,et al.  Sources, fate and effects of microplastics in the marine environment: part 2 of a global assessment , 2015 .

[58]  Richard C. Thompson,et al.  The deep sea is a major sink for microplastic debris , 2014, Royal Society Open Science.

[59]  Andrew Turner,et al.  Occurrence, distribution and characteristics of beached plastic production pellets on the island of Malta (central Mediterranean). , 2011, Marine pollution bulletin.

[60]  M. McHugh,et al.  Occurrence of microplastics in the gastrointestinal tract of pelagic and demersal fish from the English Channel. , 2013, Marine pollution bulletin.

[61]  T. Rocha-Santos,et al.  A critical overview of the analytical approaches to the occurrence, the fate and the behavior of microplastics in the environment , 2015 .