Study on Anticancer Extractions and Components from Selaginella Doederleinii Hieron

Objective To investigate the anticancer effect of extracts and reveal the active components in S.doederleinii.Methods Anticancer effect of the four extracts of ethanol,petroleum ether,dichloromethane,ethyl acetate from S.doederleinii and the residual water portion on human tumor cell lines cultured in vitro was tested by MTT assay.The active components were separated from the screened extract by preparative-HPLC and identified by MS and NMR.Results Dichloromethane and ethyl acetate extracts were found to be the effective fractions from S.doederleinii.Four compounds were separated from ethyl acetate extract and identified as amentoflavone(Ⅰ),robustflavone(Ⅱ),2″,3″-dihydro-3′,3″′-biapigenin(Ⅲ) and 3′,3″′-binaringenin(Ⅳ).Compounds Ⅲ and Ⅳ were identified from this plant for the first time.Conclusions Anticancer components of S.doederleinii may concentrated in the extracts of dichloromethane and ethyl acetate,and compounds Ⅰ,Ⅱ,Ⅲ and Ⅳ separated from ethyl acetate extract may have the most antitumor activities.