Low level chemiluminescence of the cytochrome c‐catalyzed decomposition of hydrogen peroxide

Hydroperoxides supplemented with hemoproteins are effective sources of photoemission. Hydrogen peroxide [I] and organic hydroperoxides (t-butyl, ethyl, and cumene hydroperoxide) [2] are comparatively active, though H202 shows a lo-fold higher chemiluminescence yield [ 1,2]. We have identified singlet molecular oxygen as the main chemiluminescent species in mixtures of cytochrome c with either H,O, [ 1 ] or t-butyl hydroperoxide [2]. Considering that HzOz is widely produced in cells and’tissues [3] and that its interaction with hemoproteins may well explain part of the chemiluminescence observed in isolated cells and intact organs [4-71, we report here some of the properties of the chemiluminescence of the HzO,/cytochrome c system along with the effect of cyanide, radical traps and heavy metals.

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