Peptidyl-Prolyl cis/trans Isomerase-Independent Functional NifH Mutant of Azotobacter vinelandii

ABSTRACT Peptidyl-prolyl cis/trans isomerases (PPIases) play a pivotal role in catalyzing the correct folding of many prokaryotic and eukaryotic proteins that are implicated in a variety of biological functions, ranging from cell cycle regulation to bacterial infection. The nif accessory protein NifM, which is essential for the biogenesis of a functional NifH component of nitrogenase, is a PPIase. To understand the nature of the molecular signature that defines the NifM dependence of NifH, we screened a library of nifH mutants in the nitrogen-fixing bacterium Azotobacter vinelandii for mutants that acquired NifM independence. Here, we report that NifH can acquire NifM independence when the conserved Pro258 located in the C-terminal region of NifH, which wraps around the other subunit in the NifH dimer, is replaced by serine.

[1]  M. Nathan,et al.  Cyclosporin A but not FK-506 protects against dopamine-induced apoptosis in the stunned heart. , 2005, The Annals of thoracic surgery.

[2]  P. Ludden,et al.  Maturation of Nitrogenase: a Biochemical Puzzle , 2005, Journal of bacteriology.

[3]  D. Rees,et al.  Structural Basis of Biological Nitrogen Fixation. , 1996, Chemical reviews.

[4]  Scott B. Selleck,et al.  GREAT METALLOCLUSTERS IN ENZYMOLOGY , 2005 .

[5]  J. Sowadski,et al.  Prevalent overexpression of prolyl isomerase Pin1 in human cancers. , 2004, The American journal of pathology.

[6]  J. Hacker,et al.  Biochemical and Functional Analyses of the Mip Protein: Influence of the N-Terminal Half and of Peptidylprolyl Isomerase Activity on the Virulence of Legionella pneumophila , 2003, Infection and Immunity.

[7]  Andrzej Galat,et al.  Peptidylprolyl cis/trans isomerases (immunophilins): biological diversity--targets--functions. , 2003, Current topics in medicinal chemistry.

[8]  D. Picard,et al.  The Hsp90‐binding peptidylprolyl isomerase FKBP52 potentiates glucocorticoid signaling in vivo , 2003, The EMBO journal.

[9]  J. Seymour,et al.  Legionella pneumophila mip gene sequencing to investigate a cluster of pneumonia cases , 2003, Pathology.

[10]  P. Hallenbeck,et al.  Nitrogenase mechanism: can old lags teach us new tricks? , 2002 .

[11]  L. Pulakat,et al.  Role of the NifM in maturation of the Fe-protein of nitrogenase. , 2002 .

[12]  S. Harayama,et al.  DNA shuffling and family shuffling for in vitro gene evolution. , 2002, Methods in molecular biology.

[13]  L. Pulakat,et al.  Regulated expression of the nifM of Azotobacter vinelandii in response to molybdenum and vanadium supplements in Burk's nitrogen-free growth medium. , 1999, Biochemical and biophysical research communications.

[14]  D C Rees,et al.  Conformational variability in structures of the nitrogenase iron proteins from Azotobacter vinelandii and Clostridium pasteurianum. , 1998, Journal of molecular biology.

[15]  G. Fischer,et al.  The mode of action of peptidyl prolyl cis/trans isomerases in vivo: binding vs. catalysis , 1998, FEBS letters.

[16]  A. Means,et al.  The mitotic peptidyl‐prolyl isomerase, Pin1, interacts with Cdc25 and Plx1 , 1998, The EMBO journal.

[17]  P. Ludden,et al.  ApoNifH functions in iron-molybdenum cofactor synthesis and apodinitrogenase maturation. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[18]  D. Rees,et al.  Structure of ADP·AIF4 –-stabilized nitrogenase complex and its implications for signal transduction , 1997, Nature.

[19]  B. Burgess,et al.  Mechanism of Molybdenum Nitrogenase. , 1996, Chemical reviews.

[20]  T. Hunter,et al.  A human peptidyl–prolyl isomerase essential for regulation of mitosis , 1996, Nature.

[21]  Guy Plunkett,et al.  A new family of peptidyl-prolyl isomerases. , 1995, Trends in biochemical sciences.

[22]  J. Hacker,et al.  Confirmation of the existence of a third family among peptidyl‐prolyl cis/trans isomerases Amino acid sequence and recombinant production of parvulin , 1994, FEBS letters.

[23]  W. Stemmer.  Rapid evolution of a protein in vitro by DNA shuffling , 1994, Nature.

[24]  G. Fischer,et al.  Peptidyl-prolyl cis/trans isomerases and their effectors , 1994 .

[25]  G. Fischer,et al.  A novel peptidyl‐prolyl cisltrans isomerase from Escherichia coli , 1994, FEBS letters.

[26]  G. Roberts,et al.  Dinitrogenase reductase- and MgATP-dependent maturation of apodinitrogenase from Azotobacter vinelandii. , 1993, The Journal of biological chemistry.

[27]  D. Rees,et al.  Crystallographic structure of the nitrogenase iron protein from Azotobacter vinelandii. , 1992, Science.

[28]  R. Setterquist,et al.  Physical and genetic map of the major nif gene cluster from Azotobacter vinelandii , 1989, Journal of bacteriology.

[29]  K. Bostian,et al.  Sequence and mutational analysis of ESS1, a gene essential for growth in Saccharomyces cerevisiae , 1989, Yeast.

[30]  W. Orme-Johnson,et al.  Klebsiella pneumoniae nifM gene product is required for stabilization and activation of nitrogenase iron protein in Escherichia coli. , 1986, The Journal of biological chemistry.

[31]  P. W. Wilson,et al.  Formation of the nitrogen-fixing enzyme system in Azotobacter vinelandii. , 1968, Canadian journal of microbiology.