50 Hz Extremely Low Frequency Electromagnetic Fields Enhance Protein Carbonyl Groups Content in Cancer Cells: Effects on Proteasomal Systems

Electromagnetic fields are an assessed cause of prolonging free radicals lifespan. This study was carried out to investigate the influence of extremely low frequency electromagnetic fields on protein oxidation and on the 20S proteasome functionality, the complex responsible for the degradation of oxidized proteins. Caco 2 cells were exposed, for 24–72 hours, to 1 mT, 50 Hz electromagnetic fields. The treatment induced a time-dependent increase both in cell growth and in protein oxidation, more evident in the presence of TPA, while no changes in cell viability were detected. Exposing the cells to 50 Hz electromagnetic fields caused a global activation of the 20S proteasome catalytic components, particularly evident at 72 hours exposure and in the presence of TPA. The finding that EGCG, a natural antioxidant compound, counteracted the field-related pro-oxidant effects demonstrates that the increased proteasome activity was due to an enhancement in intracellular free radicals.

[1]  M. Plummer,et al.  International agency for research on cancer. , 2020, Archives of pathology.

[2]  Tobias Jung,et al.  The proteasome and its role in the degradation of oxidized proteins , 2008, IUBMB life.

[3]  H. Raza,et al.  In Vitro Effects of Tea Polyphenols on Redox Metabolism, Oxidative Stress, and Apoptosis in PC12 Cells , 2008, Annals of the New York Academy of Sciences.

[4]  I. Jariel-Encontre,et al.  Ubiquitin-independent- versus ubiquitin-dependent proteasomal degradation of the c-Fos and Fra-1 transcription factors: is there a unique answer? , 2008, Biochimie.

[5]  R. Cervellati,et al.  Catechins: natural free-radical scavengers against ochratoxin A-induced cell damage in a pig kidney cell line (LLC-PK1). , 2007, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[6]  Kwang-Won Lee,et al.  Protective effect of (-)-epigallocatechin gallate against advanced glycation endproducts-induced injury in neuronal cells. , 2007, Biological & pharmaceutical bulletin.

[7]  C. Thompson,et al.  Apoptosis regulation by Bcl-xL modulation of mammalian inositol 1,4,5-trisphosphate receptor channel isoform gating , 2007, Proceedings of the National Academy of Sciences.

[8]  S. Srichairatanakool,et al.  Epigallocatechin-3-gallate and epicatechin-3-gallate from green tea decrease plasma non-transferrin bound iron and erythrocyte oxidative stress. , 2007, Medicinal chemistry (Shariqah (United Arab Emirates)).

[9]  F. Prato,et al.  Real‐time measurement of cytosolic free calcium concentration in Jurkat cells during ELF magnetic field exposure and evaluation of the role of cell cycle , 2006, Bioelectromagnetics.

[10]  M. Glei,et al.  The main catechin of green tea, (-)-epigallocatechin-3-gallate (EGCG), reduces bleomycin-induced DNA damage in human leucocytes. , 2006, Toxicology in vitro : an international journal published in association with BIBRA.

[11]  M. Angeletti,et al.  Effect of polyphenolic compounds on the proteolytic activities of constitutive and immuno-proteasomes. , 2006, Antioxidants & redox signaling.

[12]  M. Scarfì,et al.  Effects on apoptosis and reactive oxygen species formation by Jurkat cells exposed to 50 Hz electromagnetic fields , 2006, Bioelectromagnetics.

[13]  K. Davies,et al.  Preferential degradation of oxidized proteins by the 20S proteasome may be inhibited in aging and in inflammatory neuromuscular diseases , 2006, Neurology.

[14]  S. Grimaldi,et al.  Extremely Low Frequency 7 Hz 100 µT Electromagnetic Radiation Promotes Differentiation in the Human Epithelial Cell Line HaCaT , 2006, Electromagnetic biology and medicine.

[15]  D. G. Mita,et al.  Effect of extremely low frequency magnetic fields on calpain activation , 2006, Bioelectromagnetics.

[16]  Francesco Piva,et al.  Pro-oxidant effects of extremely low frequency electromagnetic fields in the land snail Helix aspersa. , 2005, Free radical biology & medicine.

[17]  R. Wojcikiewicz,et al.  The role of Ca2+ in triggering inositol 1,4,5-trisphosphate receptor ubiquitination. , 2005, The Biochemical journal.

[18]  M. Latocha,et al.  Effect of extremely low frequency of electromagnetic fields on cell proliferation, antioxidative enzyme activities and lipid peroxidation in 3T3-L1 preadipocytes--an in vitro study. , 2005, Journal of physiology and pharmacology : an official journal of the Polish Physiological Society.

[19]  I. Panfoli,et al.  Effects of extremely low frequency electromagnetic fields on membrane-associated enzymes. , 2005, Archives of biochemistry and biophysics.

[20]  C. Marchini,et al.  Aspirin modulates LPS-induced nitric oxide release in rat glial cells , 2005, Neuroscience Letters.

[21]  A. Boninsegna,et al.  50-Hz extremely low frequency electromagnetic fields enhance cell proliferation and DNA damage: possible involvement of a redox mechanism. , 2005, Biochimica et biophysica acta.

[22]  J. Greenleaf,et al.  Exercise training and 3-day head down bed rest deconditioning: exercise thermoregulation. , 2005, Journal of physiology and pharmacology : an official journal of the Polish Physiological Society.

[23]  R. Saunders,et al.  Static magnetic fields: animal studies. , 2005, Progress in biophysics and molecular biology.

[24]  P. Indovina,et al.  Extremely low frequency (ELF) magnetic fields and apoptosis: a review , 2005, International Journal of Radiation Biology.

[25]  Myrtill Simkó,et al.  Fifty-hertz magnetic fields induce free radical formation in mouse bone marrow-derived promonocytes and macrophages. , 2004, Biochimica et biophysica acta.

[26]  Yili Yang,et al.  Regulating the p53 system through ubiquitination , 2004, Oncogene.

[27]  Y. Reshetnyak,et al.  Conformational and enzymatic changes of 20S proteasome of rat natural killer cells induced by mono- and divalent cations. , 2004, Journal of structural biology.

[28]  H. Drexler.  The role of p27Kip1 in proteasome inhibitor induced apoptosis. , 2003, Cell cycle.

[29]  C. Hill,et al.  The pore of activated 20S proteasomes has an ordered 7‐fold symmetric conformation , 2003, The EMBO journal.

[30]  K. Davies,et al.  Selective degradation of oxidatively modified protein substrates by the proteasome. , 2003, Biochemical and biophysical research communications.

[31]  G. Eichholz.  Non-ionizing Radiation, Part 1: Static and Extremely Low-frequency (elf) Electric and Magnetic Fields, , 2002 .

[32]  D. Weiss,et al.  Influence of 50 Hz electromagnetic fields in combination with a tumour promoting phorbol ester on protein kinase C and cell cycle in human cells , 2002, Molecular and Cellular Biochemistry.

[33]  David M. Smith,et al.  Ester Bond-containing Tea Polyphenols Potently Inhibit Proteasome Activity in Vitro and in Vivo * , 2001, The Journal of Biological Chemistry.

[34]  K. Davies.  Degradation of oxidized proteins by the 20S proteasome. , 2001, Biochimie.

[35]  M. Orłowski,et al.  Catalytic activities of the 20 S proteasome, a multicatalytic proteinase complex. , 2000, Archives of biochemistry and biophysics.

[36]  Q. Dou,et al.  Bax degradation by the ubiquitin/proteasome-dependent pathway: involvement in tumor survival and progression. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[37]  M. Angeletti,et al.  Isolation and characterization of bovine thymus multicatalytic proteinase complex. , 2000, Protein expression and purification.

[38]  M. Furniss,et al.  Bruton’s Tyrosine Kinase Activity and Inositol 1,4,5-Trisphosphate Production Are Not Altered in DT40 Lymphoma B Cells Exposed to Power Line Frequency Magnetic Fields* , 1998, The Journal of Biological Chemistry.

[39]  W. Löscher,et al.  Animal and cellular studies on carcinogenic effects of low frequency (50/60-Hz) magnetic fields. , 1998, Mutation research.

[40]  M. Mattson,et al.  Lysophosphatidic Acid Induces Necrosis and Apoptosis in Hippocampal Neurons , 1998, Journal of neurochemistry.

[41]  S. Grimaldi,et al.  Effect of extremely low frequency (ELF) magnetic field exposure on morphological and biophysical properties of human lymphoid cell line (Raji). , 1997, Biochimica et biophysica acta.

[42]  Marian Orlowski,et al.  Bovine Spleen Multicatalytic Proteinase Complex (Proteasome) , 1997, The Journal of Biological Chemistry.

[43]  M. Matsuishi,et al.  Proteasome from rabbit skeletal muscle: Some properties and effects on muscle proteins. , 1997, Meat science.

[44]  L. Dick,et al.  Mechanistic Studies on the Inactivation of the Proteasome by Lactacystin , 1996, The Journal of Biological Chemistry.

[45]  R. Liburdy,et al.  The phorbol 12‐myristate 13‐ acetate (PMA)‐induced oxidative burst in rat peritoneal neutrophils is increased by a 0.1 mT (60 Hz) magnetic field , 1995, FEBS letters.

[46]  W. Foster,et al.  The reproductive toxicology of Great Lakes contaminants. , 1995, Environmental health perspectives.

[47]  R. Huber,et al.  Crystal structure of the 20S proteasome from the archaeon T. acidophilum at 3.4 A resolution. , 1995, Science.

[48]  B. Holmberg,et al.  Magnetic fields and cancer: animal and cellular evidence--an overview. , 1995, Environmental health perspectives.

[49]  J. Landgrave,et al.  A pilot plant for removing chromium from residual water of tanneries. , 1995, Environmental health perspectives.

[50]  Erik Lundgren,et al.  Intracellular calcium oscillations induced in a T‐cell line by a weak 50 Hz magnetic field , 1993, Journal of cellular physiology.

[51]  J. Scaiano,et al.  A comparative study of magnetic field effects on the dynamics of geminate and random radical pair processes in micelles , 1993 .

[52]  M Orlowski,et al.  Evidence for the presence of five distinct proteolytic components in the pituitary multicatalytic proteinase complex. Properties of two components cleaving bonds on the carboxyl side of branched chain and small neutral amino acids. , 1993, Biochemistry.

[53]  M. Orłowski,et al.  The multicatalytic proteinase complex, a major extralysosomal proteolytic system. , 1990, Biochemistry.

[54]  A. Rivett,et al.  Preferential degradation of the oxidatively modified form of glutamine synthetase by intracellular mammalian proteases. , 1985, The Journal of biological chemistry.

[55]  T. Mosmann.  Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. , 1983, Journal of immunological methods.

[56]  M. Orłowski,et al.  Evidence that Pituitary Cation‐Sensitive Neutral Endopeptidase Is a Multicatalytic Protease Complex , 1983, Journal of neurochemistry.

[57]  J. Almenoff,et al.  Membrane-bound kidney neutral metalloendopeptidase: interaction with synthetic substrates, natural peptides, and inhibitors. , 1983, Biochemistry.

[58]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[59]  G. Pfleiderer,et al.  [ISOLATION OF AN AMINOPEPTIDASE FROM KIDNEY PARTICLES]. , 1963, Biochemische Zeitschrift.

[60]  Peng Song-lin,et al.  Effect of electromagnetic fields on proliferation and differentiation of cultured mouse bone marrow mesenchymal stem cells , 2008, Journal of Huazhong University of Science and Technology [Medical Sciences].

[61]  Leeka Kheifets,et al.  EMF and health. , 2005, Annual review of public health.

[62]  T. Grune.  Oxidative stress, aging and the proteasomal system , 2004, Biogerontology.

[63]  R. Hesketh,et al.  Biological responses to electromagnetic fields , 1998 .

[64]  D. Weiss,et al.  Effects of 50 Hz EMF exposure on micronucleus formation and apoptosis in transformed and nontransformed human cell lines. , 1998, Bioelectromagnetics.

[65]  H. Lai,et al.  Acute exposure to a 60 Hz magnetic field increases DNA strand breaks in rat brain cells. , 1997, Bioelectromagnetics.

[66]  R. Astumian,et al.  Protein kinase C activity is altered in HL60 cells exposed to 60 Hz AC electric fields. , 1996, Bioelectromagnetics.

[67]  K. H. Mild,et al.  Chromosomal aberrations in human amniotic cells after intermittent exposure to fifty hertz magnetic fields. , 1994, Bioelectromagnetics.

[68]  C. Cardozo,et al.  Catalytic components of the bovine pituitary multicatalytic proteinase complex (proteasome). , 1993, Enzyme & protein.

[69]  F. James Rohlf,et al.  Biometry: The Principles and Practice of Statistics in Biological Research , 1969 .