Whole-Genome Shotgun Assembly and Analysis of the Genome of Streptomyces mobaraensis DSM 40847, a Strain for Industrial Production of Microbial Transglutaminase

ABSTRACT Here, we report the draft annotated genome sequence of Streptomyces mobaraensis strain DSM 40847, which is used in industry to produce microbial transglutaminase. The genome sequence will allow for the characterization of the molecular mechanisms underlying the beneficial properties of this organism.

[1]  S. Eddy,et al.  tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. , 1997, Nucleic acids research.

[2]  M. Motoki,et al.  Purification and characterization of a tissue-type transglutaminase from red sea bream (Pagrus major). , 1994, Bioscience, biotechnology, and biochemistry.

[3]  Zhemin Zhou,et al.  The order of expression is a key factor in the production of active transglutaminase in Escherichia coli by co-expression with its pro-peptide , 2011, Microbial cell factories.

[4]  M. Piacentini,et al.  The transglutaminase family: an overview: Minireview article , 2004, Amino Acids.

[5]  R. Duran,et al.  Purification, characterisation, and gene cloning of transglutaminase from Streptoverticillium cinnamoneum CBS 683.68. , 1998, Biochimie.

[6]  E. Birney,et al.  Velvet: algorithms for de novo short read assembly using de Bruijn graphs. , 2008, Genome research.

[7]  F. Facchiano,et al.  Transglutaminases and their substrates in biology and human diseases: 50 years of growing , 2009, Amino Acids.

[8]  M. Pietzsch,et al.  Purification and activation of a recombinant histidine-tagged pro-transglutaminase after soluble expression in Escherichia coli and partial characterization of the active enzyme , 2008 .

[9]  P. Ruggeri,et al.  Polyamines biosynthesis and oxidation in free-living amoebae , 2004, Amino Acids.

[10]  Steven Salzberg,et al.  Identifying bacterial genes and endosymbiont DNA with Glimmer , 2007, Bioinform..

[11]  K. Yokoyama,et al.  High level expression of Streptomyces mobaraensis transglutaminase in Corynebacterium glutamicum using a chimeric pro-region from Streptomyces cinnamoneus transglutaminase. , 2004, Journal of biotechnology.

[12]  Jian Chen,et al.  A temperature-shift strategy in batch microbial transglutaminase fermentation , 2001 .

[13]  E. Myers,et al.  Basic local alignment search tool. , 1990, Journal of molecular biology.

[14]  Peter F. Hallin,et al.  RNAmmer: consistent and rapid annotation of ribosomal RNA genes , 2007, Nucleic acids research.

[15]  Yoshihiro Yamanishi,et al.  KEGG for linking genomes to life and the environment , 2007, Nucleic Acids Res..

[16]  P. W. Cole,et al.  Mechanism of action of guinea pig liver transglutaminase. IV. The trimethylacyl enzyme. , 1967, The Journal of biological chemistry.

[17]  S. Chung.  COMPARATIVE STUDIES ON TISSUE TRANSGLUTAMINASE AND FACTOR XIII , 1972, Annals of the New York Academy of Sciences.

[18]  M. Borodovsky,et al.  GeneMarkS: a self-training method for prediction of gene starts in microbial genomes. Implications for finding sequence motifs in regulatory regions. , 2001, Nucleic acids research.

[19]  Michael Y. Galperin,et al.  The COG database: a tool for genome-scale analysis of protein functions and evolution , 2000, Nucleic Acids Res..

[20]  K. Kobayashi,et al.  Purification and Characterization of Novel Transglutaminase from Bacillus subtilis Spores , 2000, Bioscience, biotechnology, and biochemistry.