A Facile Fabrication of a Potentiometric Arrayed Glucose Biosensor Based on Nafion-GOx/GO/AZO

In this study, the potentiometric arrayed glucose biosensors, which were based on zinc oxide (ZnO) or aluminum-doped zinc oxide (AZO) sensing membranes, were fabricated by using screen-printing technology and a sputtering system, and graphene oxide (GO) and Nafion-glucose oxidase (GOx) were used to modify sensing membranes by using the drop-coating method. Next, the material properties were characterized by using a Raman spectrometer, a field-emission scanning electron microscope (FE-SEM), and a scanning probe microscope (SPM). The sensing characteristics of the glucose biosensors were measured by using the voltage–time (V-T) measurement system. Finally, electrochemical impedance spectroscopy (EIS) was conducted to analyze their charge transfer abilities. The results indicated that the average sensitivity of the glucose biosensor based on Nafion-GOx/GO/AZO was apparently higher than that of the glucose biosensor based on Nafion-GOx/GO/ZnO. In addition, the glucose biosensor based on Nafion-GOx/GO/AZO exhibited an excellent average sensitivity of 15.44 mV/mM and linearity of 0.997 over a narrow range of glucose concentration range, a response time of 26 s, a limit of detection (LOD) of 1.89 mM, and good reproducibility. In terms of the reversibility and stability, the hysteresis voltages (VH) were 3.96 mV and 2.42 mV. Additionally, the glucose biosensor also showed good anti-inference ability and reproducibility. According to these results, it is demonstrated that AZO is a promising material, which could be used to develop a reliable, simple, and low-cost potentiometric glucose biosensor.

[1]  B. Danielsson,et al.  An intracellular glucose biosensor based on nanoflake ZnO , 2010 .

[2]  Wei Wang,et al.  Glucose biosensor based on glucose oxidase immobilized on unhybridized titanium dioxide nanotube arrays , 2014, Microchimica Acta.

[3]  U. Tamer,et al.  An enzyme free potentiometric detection of glucose based on a conducting polymer poly (3-aminophenyl boronic acid-co-3-octylthiophene) , 2013 .

[4]  Martin Pumera,et al.  Graphene in biosensing , 2011 .

[5]  S. Adeloju,et al.  Fabrication of ultra-thin polypyrrole-glucose oxidase film from supporting electrolyte-free monomer solution for potentiometric biosensing of glucose. , 2001, Biosensors & bioelectronics.

[6]  James Alastair McLaughlin,et al.  Graphene oxide for electrochemical sensing applications , 2011 .

[7]  M. S. Akhtar,et al.  Nanocages-augmented aligned polyaniline nanowires as unique platform for electrochemical non-enzymatic glucose biosensor , 2016 .

[8]  Xin Lu,et al.  Fast and Facile Preparation of Graphene Oxide and Reduced Graphene Oxide Nanoplatelets , 2009 .

[9]  X. W. Sun,et al.  Enzymatic glucose biosensor based on ZnO nanorod array grown by hydrothermal decomposition , 2006 .

[10]  Yang Li,et al.  Electrocatalytic Oxidation of NADH on Graphene Oxide and Reduced Graphene Oxide Modified Screen-Printed Electrode , 2011, International Journal of Electrochemical Science.

[11]  T. Rocha-Santos,et al.  Graphene based sensors and biosensors , 2017 .

[12]  Jian-hui Jiang,et al.  A label-free amplified fluorescence DNA detection based on isothermal circular strand-displacement polymerization reaction and graphene oxide. , 2013, The Analyst.

[13]  Pallav Gupta,et al.  Structural and optical properties of Al & Li doped ZnO nanorods , 2018 .

[14]  Jean-Paul Mosnier,et al.  Mediator-free interaction of glucose oxidase, as model enzyme for immobilization, with Al-doped and undoped ZnO thin films laser-deposited on polycarbonate supports. , 2017, Enzyme and microbial technology.

[15]  Oh Seok Kwon,et al.  Graphene-based nanoelectronic biosensors , 2016 .

[16]  S. Usui,et al.  Electrical double-layer interaction between oppositely charged dissimilar oxide surfaces with charge regulation and Stern-Grahame layers. , 2008, Journal of colloid and interface science.

[17]  Zafar Hussain Ibupoto,et al.  Potentiometric glucose sensor based on the glucose oxidase immobilized iron ferrite magnetic particle/chitosan composite modified gold coated glass electrode , 2012 .

[18]  T. Pajkossy,et al.  Electrochemical impedance spectroscopy in interfacial studies , 2017 .

[20]  F. Wang,et al.  Enhanced amperometric response of a glucose oxidase and horseradish peroxidase based bienzyme glucose biosensor modified with a film of polymerized toluidine blue containing reduced graphene oxide , 2015, Microchimica Acta.

[21]  Wanbei Bai,et al.  Potentiometric glucose biosensor based on core-shell Fe3O4-enzyme-polypyrrole nanoparticles. , 2014, Biosensors & bioelectronics.

[22]  L. Bousse,et al.  Hysteresis in Al2O3 Gate ISFETs , 1990 .

[23]  Yan Wang,et al.  A novel bienzyme glucose biosensor based on three-layer Au–Fe3O4@SiO2 magnetic nanocomposite , 2011 .

[24]  P Atanasov,et al.  Integrated implantable device for long-term glucose monitoring. , 1995, Biosensors & bioelectronics.

[25]  Magnus Willander,et al.  A fast and sensitive potentiometric glucose microsensor based on glucose oxidase coated ZnO nanowires grown on a thin silver wire , 2010 .

[26]  Santosh K. Misra,et al.  Electrochemical-digital immunosensor with enhanced sensitivity for detecting human salivary glucocorticoid hormone. , 2018, The Analyst.

[27]  Vinay Gupta.  ZnO based third generation biosensor , 2010 .

[28]  Caiping Yang,et al.  Synthesis and characterization of MgO/ZnO composite nanosheets for biosensor , 2015 .

[29]  Davor Z Antanasijević,et al.  Review: the approaches for estimation of limit of detection for ICP-MS trace analysis of arsenic. , 2012, Talanta.

[30]  Dipanjan Pan,et al.  Electrically-receptive and thermally-responsive paper-based sensor chip for rapid detection of bacterial cells. , 2018, Biosensors & bioelectronics.

[31]  Jacek Klinowski,et al.  A new structural model for graphite oxide , 1998 .

[32]  O. Nur,et al.  Zinc oxide nano-rods based glucose biosensor devices fabrication , 2018, Results in Physics.

[33]  Jian Liu,et al.  Polyaniline-based glucose biosensor: A review , 2016 .

[34]  Jianrong Chen,et al.  Amperometric glucose sensor based on enhanced catalytic reduction of oxygen using glucose oxidase adsorbed onto core-shell Fe3O4@silica@Au magnetic nanoparticles. , 2012, Materials science & engineering. C, Materials for biological applications.

[35]  K. Kleppe,et al.  THE OXIDATION OF GLUCOSE AND RELATED COMPOUNDS BY GLUCOSE OXIDASE FROM ASPERGILLUS NIGER. , 1964, Biochemistry.

[36]  J. Ghosh,et al.  Tuning the visible photoluminescence in Al doped ZnO thin film and its application in label-free glucose detection , 2018 .

[37]  Jung-Chuan Chou,et al.  Data Fusion and Fault Diagnosis for Flexible Arrayed pH Sensor Measurement System Based on LabVIEW , 2014, IEEE Sensors Journal.

[38]  Suprijadi,et al.  Preliminary Study of Molecularly Imprinted Polymer-based Potentiometric Sensor for Glucose☆ , 2017 .

[39]  Novel potentiometric approach in glucose biosensor using silver nanoparticles as redox marker , 2009 .

[40]  Xiaohua Zhu,et al.  An amperometric glucose biosensor based on the immobilization of glucose oxidase on the platinum electrode modified with NiO doped ZnO nanorods , 2012 .

[41]  Agis A. Iliadis,et al.  Properties of high sensitivity ZnO surface acoustic wave sensors on SiO2/(100) Si substrates , 2008 .

[42]  Yong Huang,et al.  Non-enzymatic electrochemical hydrogen peroxide biosensor based on reduction graphene oxide-persimmon tannin‑platinum nanocomposite. , 2018, Materials science & engineering. C, Materials for biological applications.

[43]  Danila Moscone,et al.  Electrochemical biosensors based on nanomodified screen-printed electrodes: Recent applications in clinical analysis , 2016 .

[44]  G. Dresselhaus,et al.  Raman spectroscopy as a probe of graphene and carbon nanotubes , 2008, Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences.

[45]  Sandeep Kumar Vashist,et al.  Technology behind commercial devices for blood glucose monitoring in diabetes management: a review. , 2011, Analytica chimica acta.

[46]  Anderson Janotti,et al.  Fundamentals of zinc oxide as a semiconductor , 2009 .

[47]  Burak Derkus,et al.  Applying the miniaturization technologies for biosensor design. , 2016, Biosensors & bioelectronics.

[48]  Jian Xie,et al.  An aqueous media based approach for the preparation of a biosensor platform composed of graphene oxide and Pt-black. , 2012, Biosensors & bioelectronics.

[49]  M. Dresselhaus,et al.  Studying disorder in graphite-based systems by Raman spectroscopy. , 2007, Physical chemistry chemical physics : PCCP.

[50]  Zhiqiang Gao,et al.  An interference-free glucose biosensor based on a novel low potential redox polymer mediator , 2014 .

[51]  G. S. Wilson,et al.  Electrochemical Biosensors: Recommended Definitions and Classification , 1999, Biosensors & bioelectronics.

[52]  Mireia Baeza,et al.  Trends in electrochemical impedance spectroscopy involving nanocomposite transducers: Characterization, architecture surface and bio-sensing , 2017 .

[53]  Yanbin Wang,et al.  Self-assembly of mildly reduced graphene oxide monolayer for enhanced Raman scattering , 2016 .

[54]  Tongyu Wu,et al.  The Characteristic Analysis of IGZO/Al pH Sensor and Glucose Biosensor in Static and Dynamic Measurements , 2016, IEEE Sensors Journal.

[55]  Abdulazeez T. Lawal.  Progress in utilisation of graphene for electrochemical biosensors. , 2018, Biosensors & bioelectronics.