Macromolecular syntheses and mitosis in UV-irradiated plasmodia of Physarum polycephalum.

In the multinucleated plasmodia of the myxomycete Physarum polycephalum, irradiation with ultraviolet light delays the first postirradiation mitosis and the intervals between several subsequent mitoses are shorter than in unirradiated controls. In order to obtain more information on the factors involved, plasmodia were placed, at different times after irradiation, on media containing actinomycin (inhibition of RNA synthesis), cycloheximide (inhibition of protein synthesis), 5-fluoro-2'-deoxyuridine (inhibition of DNA synthesis), or nonnutrient balanced salt solution (total starvation). Sensitivity of the plasmodia to mitotic delay produced by actinomycin decreased rapidly after uv irradiation and reached approximately zero at the time when the nuclei would have divided if the plasmodia had not been irradiated. Following the first postirradiation mitosis, the plasmodia remained almost insensitive to continuous exposure to actinomycin for several mitotic cycles. Starvation of the irradiated plasmodia by tra...

[1]  R. Painter,et al.  RADIATION-STIMULATED DNA SYNTHESIS IN CULTURED MAMMALIAN CELLS , 1966, The Journal of cell biology.

[2]  D. Woolley.  A study of antimetabolites , 1952 .

[3]  P. Hanawalt,et al.  The nature of the excision-repair region in the DNA from a eucaryotic organism, Tetrahymena pyriformis. , 1969, Radiation research.

[4]  J. Bonner,et al.  Histone-bound RNA, a component of native nucleohistone. , 1965, Proceedings of the National Academy of Sciences of the United States of America.

[5]  C. Mittermayer,et al.  THE EFFECT OF ACTINOMYCIN D ON THE TIMING OF MITOSIS IN PHYSARUM POLYCEPHALUM. , 1965, Experimental cell research.

[6]  David M. Prescott,et al.  Methods in cell physiology , 1964 .

[7]  W. Sachsenmaier,et al.  THE EFFECT OF 5-FLUORO-2'-DEOXYURIDINE ON SYNCHRONOUS MITOSIS IN PHYSARUM POLYCEPHALUM. , 1964, Experimental cell research.

[8]  M. Gontcharoff,et al.  Functionality of newly synthesized DNA as related to RNA synthesis during mitotic cycle in Physarum polycephalum. , 1969, Experimental cell research.

[9]  C. Mittermayer,et al.  RNA SYNTHESIS IN THE MITOTIC CYCLE OF PHYSARUM POLYCEPHALUM. , 1964, Biochimica et biophysica acta.

[10]  P. Hanawalt,et al.  Mitochondrial DNA synthesis and the mitotic cycle in Physarum polycephalum. , 1967, Biochimica et biophysica acta.

[11]  C. Mittermayer,et al.  Ribonucleic acid synthesis in vitro in nuclei isolated from the synchronously dividing Physarum polycephalum. , 1966, Biochimica et biophysica acta.

[12]  H. P. Rusch,et al.  Effect of elevated temperature shocks on mitosis and on the initiation of DNA replication in Physarum polycephalum. , 1968, Experimental cell research.

[13]  M. Lubin,et al.  Cycloheximide: Aspects of Inhibition of Protein Synthesis in Mammalian Cells , 1964, Science.

[14]  E. Guttes,et al.  Effects of ultraviolet light on mitosis in Physarum polycephalum. , 1968, Experimental cell research.

[15]  E. Guttes,et al.  REPLICATION OF NUCLEOLUS-ASSOCIATED DNA DURING "G2 PHASE" IN PHYSARUM POLYCEPHALUM , 1969, The Journal of cell biology.

[16]  R. Setlow,et al.  Effects of ultraviolet radiation on macromolecular synthesis in Escherichia coli. , 1966, Journal of molecular biology.

[17]  W. Sachsenmaier,et al.  Advanced Initiation of Synchronous Mitoses in Physarum polycephalum Following UV-irradiation , 1970, Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete.

[18]  E. Guttes,et al.  Chapter 3 Mitotic Synchrony in the Plasmodia of Physarum polycephalum and Mitotic Synchronization by Coalescence of Microplasmodia , 1964 .

[19]  D. McConnell,et al.  Chromosomal ribonucleic acid of rat ascites cells. , 1969, Biochemistry.

[20]  J. Cleaver,et al.  Repair replication, unscheduled DNA synthesis, and the repair of mammalian DNA. , 1969, Radiation research.

[21]  H. P. Rusch,et al.  LIMITED DNA SYNTHESIS IN THE ABSENCE OF PROTEIN SYNTHESIS IN PHYSARUM POLYCEPHALUM , 1966, The Journal of cell biology.

[22]  D. Prescott,et al.  An ultraviolet microbeam study of the roles of nucleus and cytoplasm in division delay, killing, and photoreactivation of Amoeba proteus. , 1969, Experimental cell research.

[23]  H. P. Rusch,et al.  THE EFFECT OF ACTIDIONE ON MITOSIS IN THE SLIME MOLD PHYSARUM POLYCEPHALUM , 1965, The Journal of cell biology.

[24]  R. Setlow,et al.  Ultraviolet inactivation of DNA primer activity. I. Effects of different wavelengths and doses. , 1963, Biochimica et biophysica acta.