Development of a multiarray biosensor for DNA diagnostics

Abstract This work involves the development and evaluation of a multiarray biosensor for DNA diagnostics. The evaluation of various system components developed for the biosensor is discussed. The DNA probes labeled with visible and near infrared (NIR) dyes are evaluated. The detection system uses a two-dimensional charge-coupled device (CCD). Examples of application of gene probes in DNA hybridization experiments and in biomedical diagnosis (detection of the p53 cancer gene) are presented to illustrate the usefulness and potential of the biosensor device.

[1]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[2]  R. Atlas,et al.  Polymerase chain reaction: applications in environmental microbiology. , 1991, Annual Review of Microbiology.

[3]  D. Squirrell,et al.  Gene probe assays on a fibre-optic evanescent wave biosensor. , 1992, Biosensors & bioelectronics.

[4]  T. Vo-Dinh,et al.  Intensified Charge Coupled Device-Based Fiber-Optic Monitor for Rapid Remote Surface-Enhanced Raman Scattering Sensing , 1992 .

[5]  Ronald W. Davis,et al.  Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray , 1995, Science.

[6]  T. Vo-Dinh,et al.  Antibody-Based Fiberoptics Biosensor for the Carcinogen Benzo(a)pyrene , 1987 .

[7]  R. Willson,et al.  Monitoring of oligonucleotide hybridization using light-addressable potentiometric and evanescent wave fluorescence sensing , 1994 .

[8]  T. Vo‐Dinh,et al.  Surface-enhanced Raman gene probes. , 1994, Analytical chemistry.

[9]  K. Mullis,et al.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. , 1988, Science.

[10]  T. Vo‐Dinh,et al.  Synchronous luminescence: a new detection technique for multiple fluorescent probes used for DNA sequencing. , 1994, BioTechniques.

[11]  Ulrich J. Krull,et al.  Fiber optic biosensor for fluorimetric detection of DNA hybridization , 1994 .

[12]  R. Reich,et al.  A microchip for quantitative detection of molecules utilizing luminescent and radioisotope reporter groups. , 1994, BioTechniques.