Synchronized manipulation and force measurement by optical tweezers using high-speed laser scanning

Laser micromanipulation is suitable for non-contact manipulation of a small object, such as a bacteria and microbe, at the closed space. We proposed synchronized laser micromanipulation (SLM) for the trajectory control of the multiple targets by the single laser. We used the laser scanning system for scanning of the focal point of laser in high speed to realize simultaneous trajectory control of multiple objects. Condition for secure transportation of multiple objects is derived analytically and verified through the experiment. We used SLM for indirect manipulation of the microbe by the microtools, which are directly manipulated by SLM. Here we propose the force measurement method using SLM. Recognition of the microtool is improved by using a fluorescent microbead as a microtool.

[1]  Steven M. Block,et al.  Optical trapping of metallic Rayleigh particles. , 1994, Optics letters.

[2]  Fumihito Arai,et al.  Flow pattern control by flow balancing In microchannel for high speed & High Purity Separation of Microbe in Microchip , 2001 .

[3]  A. Ashkin.  Forces of a single-beam gradient laser trap on a dielectric sphere in the ray optics regime. , 1992, Methods in cell biology.

[4]  S. Chu,et al.  Observation of a single-beam gradient force optical trap for dielectric particles. , 1986, Optics letters.

[5]  Alan P. Morrison,et al.  Development of a microfluidic device for fluorescence activated cell sorting , 2002 .

[6]  Alex Terray,et al.  Microfluidic Control Using Colloidal Devices , 2002, Science.

[7]  A. Ashkin,et al.  Optical trapping and manipulation of viruses and bacteria. , 1987, Science.

[8]  S. Block,et al.  Construction of multiple-beam optical traps with nanometer-resolution position sensing , 1996 .

[9]  Fumihito Arai,et al.  Pinpoint injection of microtools for minimally invasive micromanipulation of microbe by laser trap , 2003 .

[10]  M. Berns,et al.  Wavelength dependence of cell cloning efficiency after optical trapping. , 1996, Biophysical journal.

[11]  Pieter Telleman,et al.  Rare Event Cell Surging in a Microfluidic System for Application in Prenatal Diagnosis , 1998 .

[12]  Fumihito Arai,et al.  High speed random separation of microobject in microchip by laser manipulator and dielectrophoresis , 2000, Proceedings IEEE Thirteenth Annual International Conference on Micro Electro Mechanical Systems (Cat. No.00CH36308).

[13]  Fumihito Arai,et al.  Screening of single Escherichia coli in a microchannel system by electric field and laser tweezers , 1998 .

[14]  T. Katsuragi.  Selection of Microorganisms with Specific Characteristics by Single-Cell Sorting with Flow or Microscope-Based (Including Laser Scanning) Cytometry , 2000 .

[15]  G. Fuhr,et al.  Paired microelectrode system: dielectrophoretic particle sorting and force calibration , 1999 .

[16]  Hiroshi Masuhara,et al.  Multibeam laser manipulation and fixation of microparticles , 1992 .

[17]  G. Spalding,et al.  Computer-generated holographic optical tweezer arrays , 2000, cond-mat/0008414.

[18]  W. Benecke,et al.  Linear motion of dielectric particles and living cells in microfabricated structures induced by traveling electric fields , 1991, [1991] Proceedings. IEEE Micro Electro Mechanical Systems.

[19]  Fumihito Arai,et al.  Synchronized laser micromanipulation of microtools for assembly of microbeads and indirect manipulation of microbe , 2003, Proceedings 2003 IEEE/RSJ International Conference on Intelligent Robots and Systems (IROS 2003) (Cat. No.03CH37453).

[20]  Myron R. Melamed,et al.  Flow Cytometry and Sorting, Second Edition , 1991 .

[21]  David W. M. Marr,et al.  Optical Trapping for the Manipulation of Colloidal Particles , 2000 .

[22]  Fumihito Arai,et al.  High‐speed separation system of randomly suspended single living cells by laser trap and dielectrophoresis , 2001, Electrophoresis.

[23]  H. Misawa,et al.  Pattern formation and flow control of fine particles by laser-scanning micromanipulation. , 1991, Optics letters.