Acidobacteria Phylum Sequences in Uranium-Contaminated Subsurface Sediments Greatly Expand the Known Diversity within the Phylum

ABSTRACT The abundance and composition of bacteria of the phylum Acidobacteria were surveyed in subsurface sediments from uranium-contaminated sites using amplification of 16S rRNA genes followed by clone/sequence analysis. Analysis of sequences from this study and public databases produced a revised and greatly expanded phylogeny of the Acidobacteria phylum consisting of 26 subgroups.

[1]  C. Kuske,et al.  Environmental Survey for Four Pathogenic Bacteria and Closely Related Species Using Phylogenetic and Functional Genes * , 2006, Journal of forensic sciences.

[2]  P. Janssen Identifying the Dominant Soil Bacterial Taxa in Libraries of 16S rRNA and 16S rRNA Genes , 2006, Applied and Environmental Microbiology.

[3]  W. Ludwig,et al.  Detection and Phylogenetic Relationships of Highly Diverse Uncultured Acidobacterial Communities in Altamira Cave Using 23S rRNA Sequence Analyses , 2005 .

[4]  Philip E. Long,et al.  Microbiological and Geochemical Heterogeneity in an In Situ Uranium Bioremediation Field Site , 2005, Applied and Environmental Microbiology.

[5]  Jizhong Zhou,et al.  Impacts on microbial communities and cultivable isolates from groundwater contaminated with high levels of nitric acid-uranium waste. , 2005, FEMS microbiology ecology.

[6]  James R. Cole,et al.  The Ribosomal Database Project (RDP-II): sequences and tools for high-throughput rRNA analysis , 2004, Nucleic Acids Res..

[7]  Robert T. Anderson,et al.  Resistance of Solid-Phase U(VI) to Microbial Reduction during In Situ Bioremediation of Uranium-Contaminated Groundwater , 2004, Applied and Environmental Microbiology.

[8]  D. Balkwill,et al.  Change in Bacterial Community Structure during In Situ Biostimulation of Subsurface Sediment Cocontaminated with Uranium and Nitrate , 2004, Applied and Environmental Microbiology.

[9]  D. Watson,et al.  In situ bioreduction of technetium and uranium in a nitrate-contaminated aquifer. , 2004, Environmental science & technology.

[10]  D. Balkwill,et al.  Enumeration and Characterization of Iron(III)-Reducing Microbial Communities from Acidic Subsurface Sediments Contaminated with Uranium(VI) , 2003, Applied and Environmental Microbiology.

[11]  Donald R. Metzler,et al.  Stimulating the In Situ Activity of Geobacter Species To Remove Uranium from the Groundwater of a Uranium-Contaminated Aquifer , 2003, Applied and Environmental Microbiology.

[12]  Christian Wolkersdorfer,et al.  Uranium in the Aquatic Environment , 2002 .

[13]  J. Raff,et al.  Bacterial communities in uranium mining waste piles and their interaction with heavy metals , 2002 .

[14]  Jizhong Zhou,et al.  Microbiological Characteristics in a Zero-Valent Iron Reactive Barrier , 2002, Environmental monitoring and assessment.

[15]  Jizhong Zhou,et al.  Simultaneous Recovery of RNA and DNA from Soils and Sediments , 2001, Applied and Environmental Microbiology.

[16]  C. Kuske,et al.  Wide Distribution and Diversity of Members of the Bacterial Kingdom Acidobacterium in the Environment , 1999, Applied and Environmental Microbiology.

[17]  Philip Hugenholtz,et al.  Impact of Culture-Independent Studies on the Emerging Phylogenetic View of Bacterial Diversity , 1998, Journal of bacteriology.

[18]  C. Kuske,et al.  Diverse uncultivated bacterial groups from soils of the arid southwestern United States that are present in many geographic regions , 1997, Applied and environmental microbiology.

[19]  K. Schleifer,et al.  Detection and in situ identification of representatives of a widely distributed new bacterial phylum. , 1997, FEMS microbiology letters.

[20]  N. Pace,et al.  Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. , 1985, Proceedings of the National Academy of Sciences of the United States of America.