Molecular Characterization of the Trichomonas gallinae Morphologic Complex in the United States

Abstract Forty-two Trichomonas gallinae isolates were molecularly characterized to determine whether isolates differed in genetic sequence of multiple gene targets depending on host species or geographical location. The 5.8S ribosomal RNA (rRNA) and flanking internal transcribed spacer (ITS) gene regions were amplified by polymerase chain reaction, and the sequences were analyzed phylogenetically. The results of the sequence analysis strongly suggest at least 2 species may exist within the T. gallinae morphologic complex. Based on ITS sequences, one group demonstrated high nucleotide identity to the 3 T. gallinae sequences available in GenBank, whereas the second group was more closely related to T. vaginalis (98%) than to T. gallinae (92%). Two common ground-dove (Columbina passerina) isolates shared a 95% identity with T. vaginalis and a 92% identity with T. gallinae and T. tenax. Sequence analysis of both the 18S rRNA and α-tubulin genes from a subset of the isolates supports the 5.8S-ITS sequence results. All of the T. vaginalis-like isolates originated from Arizona, California, or Texas, whereas T. gallinae isolates were found in all sampled states. Both T. vaginalis-like and T. gallinae isolates were involved in trichomoniasis outbreaks in California and Arizona.

[1]  B. Lawson,et al.  Necrotic ingluvitis in wild finches , 2005, Veterinary Record.

[2]  D. Forrester,et al.  INTERRELATIONSHIPS OF PARASITES OF WHITE-WINGED DOVES AND MOURNING DOVES IN FLORIDA1 , 1981, Journal of Wildlife Diseases.

[3]  D. Bell,et al.  Molecular identity and heterogeneity of Trichomonad parasites in a closed avian population. , 2007, Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases.

[4]  C. Jones,et al.  Comparison of the InPouch TF Culture System and Wet-Mount Microscopy for Diagnosis of Trichomonas gallinae Infections in the Pink Pigeon Columba mayeri , 2005, Journal of Clinical Microbiology.

[5]  J. Felsenstein.  CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP , 1985, Evolution; international journal of organic evolution.

[6]  S. Bonatto,et al.  Molecular phylogeny of Trichomonadidae family inferred from ITS-1, 5.8S rRNA and ITS-2 sequences. , 2004, International journal for parasitology.

[7]  D. Mead,et al.  Necropsy Findings and Arbovirus Surveillance in Mourning Doves from the Southeastern United States , 2007, Journal of wildlife diseases.

[8]  R. W. Mannan,et al.  TRICHOMONIASIS IN COOPER'S HAWKS FROM ARIZONA , 1998, Journal of wildlife diseases.

[9]  R. Stabler,et al.  Pathogenicity transformation of Trichomonas gallinae. I. Effects of homogenates and of mixtures of DNA and RNA from a virulent strain on pathogenicity of an avirulent strain. , 1971, The Journal of parasitology.

[10]  F. A. Hayes,et al.  STUDIES ON ENDOPARASITES OF THE MOURNING DOVE (Zenaida macroura) IN THE SOUTHEAST UNITED STATES1 , 1977, Journal of wildlife diseases.

[11]  C. Gortázar,et al.  Trichomonas gallinae in wintering Common Wood Pigeons Columba palumbus in Spain , 2006 .

[12]  S. Mañosa,et al.  TRICHOMONIASIS IN A BONELLI'S EAGLE POPULATION IN SPAIN , 2000, Journal of wildlife diseases.

[13]  C. Jones,et al.  Epidemiology and conservation implications of Trichomonas gallinae infection in the endangered Mauritian pink pigeon , 2008 .

[14]  Christina M Romagosa,et al.  ESTABLISHMENT AND DISPERSAL OF THE EURASIAN COLLARED-DOVE IN FLORIDA , 2000 .

[15]  T. Work,et al.  CAUSES OF OWL MORTALITY IN HAWAII, 1992 TO 1994 , 1996, Journal of wildlife diseases.

[16]  M. Sogin,et al.  Evolutionary relationships among "jakobid" flagellates as indicated by alpha- and beta-tubulin phylogenies. , 2001, Molecular biology and evolution.

[17]  M. Spalding,et al.  Parasites and Diseases of Wild Birds in Florida , 2003 .

[18]  J. Thompson,et al.  CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. , 1994, Nucleic acids research.

[19]  A. Bhattacharya,et al.  Inter- and intra-strain variation in the 5.8S ribosomal RNA and internal transcribed spacer sequences of Entamoeba histolytica and comparison with Entamoeba dispar, Entamoeba moshkovskii and Entamoeba invadens. , 2000, International journal for parasitology.

[20]  K. Reece,et al.  Molecular, Morphological, and Experimental Evidence Support the Synonymy of Perkinsus chesapeaki and Perkinsus andrewsi , 2005, The Journal of eukaryotic microbiology.

[21]  David D. Dolton,et al.  Mourning Dove Population Status, 2007 , 2002 .

[22]  John H. Schulz,et al.  Monitoring Presence and Annual Variation of Trichomoniasis in Mourning Doves , 2005, Avian diseases.

[23]  R. Felleisen.  Comparative sequence analysis of 5·8S rRNA genes and internal transcribed spacer (ITS) regions of trichomonadid protozoa , 1997, Parasitology.

[24]  C. A. Hedlund.  Trichomonas gallinae in avian populations in urban Tucson, Arizona , 1998 .

[25]  J. Flegr,et al.  New evolutionary lineages, unexpected diversity, and host specificity in the parabasalid genus Tetratrichomonas. , 2006, Molecular phylogenetics and evolution.

[26]  Stabler Rm.  A survey of colorado band-tailed pigeons, mourning doves, and wild common pigeons for Trichomonas gallinae. , 1951 .

[27]  R. W. Mannan,et al.  FEEDING BEHAVIOR OF COOPER'S HAWKS AT URBAN AND RURAL NESTS IN SOUTHEASTERN ARIZONA , 2003 .

[28]  K. Reece,et al.  Multiple Polymorphic Sites at the ITS and ATAN Loci in Cultured Isolates of Perkinsus marinus , 2004, The Journal of eukaryotic microbiology.

[29]  D. Rupiper,et al.  Prevalence of Trichomonas gallinae in central California mourning doves. , 1988 .

[30]  S. Rosenstock,et al.  Studies of Wildlife Water Developments in Southwestern Arizona: Wildlife Use,Water Quality,Wildlife Diseases,Wildlife Mortalities, and Influences on Native Pollinators , 2004 .

[31]  H. Gerlach,et al.  Pigeon Health and Disease , 1991 .

[32]  C. M. Herman,et al.  Upper digestive tract trichomoniasis in mourning doves and other birds , 1951 .

[33]  J. Flegr,et al.  Cryptic species within the Tetratrichomonas gallinarum species complex revealed by molecular polymorphism. , 2005, Veterinary parasitology.

[34]  R. Stabler.  Trichomonas gallinae:a review. , 1954, Experimental parasitology.

[35]  M. Nei,et al.  MEGA: Molecular Evolutionary Genetics Analysis, Version 1.02. , 1995 .

[36]  J. B. Jensen,et al.  Lumen Dwelling Protozoa: Entamoeba, Trichomonads, And Giardia , 1983, In Vitro Cultivation of Protozoan Parasites.