Highly Elastic and Conductive Human‐Based Protein Hybrid Hydrogels

A highly elastic hybrid hydrogel of methacryloyl-substituted recombinant human tropoelastin (MeTro) and graphene oxide (GO) nanoparticles are developed. The synergistic effect of these two materials significantly enhances both ultimate strain (250%), reversible rotation (9700°), and the fracture energy (38.8 ± 0.8 J m(-2) ) in the hybrid network. Furthermore, improved electrical signal propagation and subsequent contraction of the muscles connected by hybrid hydrogels are observed in ex vivo tests.

[1]  Cheng-Chih Hsu,et al.  Rapid self-healing hydrogels , 2012, Proceedings of the National Academy of Sciences.

[2]  Nasim Annabi,et al.  The fabrication of elastin-based hydrogels using high pressure CO(2). , 2009, Biomaterials.

[3]  Jian Ping Gong,et al.  Why are double network hydrogels so tough , 2010 .

[4]  Lisa E. Freed,et al.  Accordion-Like Honeycombs for Tissue Engineering of Cardiac Anisotropy , 2008, Nature materials.

[5]  A. Khademhosseini,et al.  Highly Elastic Micropatterned Hydrogel for Engineering Functional Cardiac Tissue , 2013, Advanced functional materials.

[6]  Séverine Rose,et al.  Nano-hybrid self-crosslinked PDMA/silica hydrogels , 2010 .

[7]  R. Langer,et al.  A tough biodegradable elastomer , 2002, Nature Biotechnology.

[8]  Yonggang Huang,et al.  Waterproof AlInGaP optoelectronics on stretchable substrates with applications in biomedicine and robotics. , 2010, Nature materials.

[9]  A. Weiss,et al.  Synthetic elastin hydrogels derived from massive elastic assemblies of self-organized human protein monomers. , 2004, Biomaterials.

[10]  T. Sun,et al.  A phase diagram of neutral polyampholyte - from solution to tough hydrogel. , 2013, Journal of materials chemistry. B.

[11]  Qizhi Chen,et al.  Elastomeric biomaterials for tissue engineering , 2013 .

[12]  Gen Kamita,et al.  Lamellar Bilayers as Reversible Sacrificial Bonds To Toughen Hydrogel: Hysteresis, Self-Recovery, Fatigue Resistance, and Crack Blunting , 2011 .

[13]  Masaru Yoshida,et al.  High-water-content mouldable hydrogels by mixing clay and a dendritic molecular binder , 2010, Nature.

[14]  A. Khademhosseini,et al.  Cell-laden microengineered gelatin methacrylate hydrogels. , 2010, Biomaterials.

[15]  Steven G Wise,et al.  Tropoelastin--a multifaceted naturally smart material. , 2013, Advanced drug delivery reviews.

[16]  Lu Shen,et al.  Preparation and mechanical properties of chitosan/carbon nanotubes composites. , 2005, Biomacromolecules.

[17]  Liqun Zhang,et al.  Elastomeric composites based on carbon nanomaterials , 2015, Nanotechnology.

[18]  Nasim Annabi,et al.  Synthesis of highly porous crosslinked elastin hydrogels and their interaction with fibroblasts in vitro. , 2009, Biomaterials.

[19]  A. Khademhosseini,et al.  Cell‐laden Microengineered and Mechanically Tunable Hybrid Hydrogels of Gelatin and Graphene Oxide , 2013, Advanced materials.

[20]  Ali Khademhosseini,et al.  Engineered cell-laden human protein-based elastomer. , 2013, Biomaterials.

[21]  Chunhai Fan,et al.  Distribution and biocompatibility studies of graphene oxide in mice after intravenous administration , 2011 .

[22]  Eric J Beckman,et al.  Synthesis of biocompatible segmented polyurethanes from aliphatic diisocyanates and diurea diol chain extenders. , 2005, Acta biomaterialia.

[23]  Cai‐Feng Wang,et al.  Robust Self-Healing Hydrogels Assisted by Cross-Linked Nanofiber Networks , 2013, Scientific Reports.

[24]  A. Khademhosseini,et al.  Carbon nanotube reinforced hybrid microgels as scaffold materials for cell encapsulation. , 2012, ACS nano.

[25]  D. Tuncaboylu,et al.  Tough and Self-Healing Hydrogels Formed via Hydrophobic Interactions , 2011 .

[26]  Wei Liu,et al.  Protein Binding by Functionalized Multiwalled Carbon Nanotubes Is Governed by the Surface Chemistry of Both Parties and the Nanotube Diameter , 2008 .

[27]  A. Khademhosseini,et al.  Regulating Cellular Behavior on Few‐Layer Reduced Graphene Oxide Films with Well‐Controlled Reduction States , 2012 .

[28]  L. Setton,et al.  Rapid cross-linking of elastin-like polypeptides with (hydroxymethyl)phosphines in aqueous solution. , 2007, Biomacromolecules.

[29]  A. Weiss,et al.  Coacervation characteristics of recombinant human tropoelastin. , 1997, European journal of biochemistry.

[30]  Matthias P. Lutolf,et al.  Designing materials to direct stem-cell fate , 2009, Nature.

[31]  K. Ito,et al.  The Polyrotaxane Gel: A Topological Gel by Figure‐of‐Eight Cross‐links , 2001 .

[32]  Toru Takehisa,et al.  Nanocomposite Hydrogels: A Unique Organic–Inorganic Network Structure with Extraordinary Mechanical, Optical, and Swelling/De‐swelling Properties , 2002 .

[33]  Itsuro Kajiwara,et al.  Mechano-actuated ultrafast full-colour switching in layered photonic hydrogels , 2014, Nature Communications.

[34]  A. Lichtenberg,et al.  The degeneration of biological cardiovascular prostheses under pro-calcific metabolic conditions in a small animal model. , 2014, Biomaterials.

[35]  James C. Weaver,et al.  Substrate stress relaxation regulates cell spreading , 2015, Nature Communications.

[36]  K. Yutzey,et al.  Placement of an elastic biodegradable cardiac patch on a subacute infarcted heart leads to cellularization with early developmental cardiomyocyte characteristics. , 2012, Journal of cardiac failure.

[37]  Hisashi Tanimoto,et al.  Self-healing in nanocomposite hydrogels. , 2011, Macromolecular rapid communications.

[38]  Akhilesh K Gaharwar,et al.  Transparent, elastomeric and tough hydrogels from poly(ethylene glycol) and silicate nanoparticles. , 2011, Acta biomaterialia.

[39]  S. Ganta,et al.  Synthesis and preliminary in vivo evaluations of polyurethane microstructures for transdermal drug delivery , 2012, Chemistry Central Journal.

[40]  Pak Kin Wong,et al.  Probing Mechanoregulation of Neuronal Differentiation by Plasma Lithography Patterned Elastomeric Substrates , 2014, Scientific Reports.

[41]  A. Thomas,et al.  The strength of highly elastic materials , 1967, Proceedings of the Royal Society of London. Series A. Mathematical and Physical Sciences.

[42]  Tal Dvir,et al.  Nanowired three dimensional cardiac patches , 2011, Nature nanotechnology.

[43]  Dragana L. Žugić,et al.  High strength thermoresponsive semi-IPN hydrogels reinforced with nanoclays , 2012 .

[44]  Z. Suo,et al.  Highly stretchable and tough hydrogels , 2012, Nature.

[45]  Zhuang Liu,et al.  Nano-graphene oxide for cellular imaging and drug delivery , 2008, Nano research.

[46]  Babak Ziaie,et al.  Biodegradable Nanofibrous Polymeric Substrates for Generating Elastic and Flexible Electronics , 2014, Advanced materials.

[47]  A. Oberhauser,et al.  Shape of tropoelastin, the highly extensible protein that controls human tissue elasticity , 2011, Proceedings of the National Academy of Sciences.

[48]  A. Weiss,et al.  Primary human dermal fibroblast interactions with open weave three-dimensional scaffolds prepared from synthetic human elastin. , 2009, Biomaterials.

[49]  Takao Someya,et al.  Ultrathin, highly flexible and stretchable PLEDs , 2013, Nature Photonics.

[50]  Ashutosh Chilkoti,et al.  Elastin‐like polypeptides: Biomedical applications of tunable biopolymers , 2010, Biopolymers.

[51]  Yen Wei,et al.  Mouldable liquid-crystalline elastomer actuators with exchangeable covalent bonds. , 2014, Nature materials.

[52]  G. Wallace,et al.  Carbon‐Nanotube Biofibers , 2007 .

[53]  F. Barth,et al.  Biomaterial systems for mechanosensing and actuation , 2009, Nature.

[54]  Jian Ping Gong,et al.  Physical hydrogels composed of polyampholytes demonstrate high toughness and viscoelasticity. , 2013, Nature materials.

[55]  Yanli Chang,et al.  In vitro toxicity evaluation of graphene oxide on A549 cells. , 2011, Toxicology letters.

[56]  Ralph Spolenak,et al.  Stretchable heterogeneous composites with extreme mechanical gradients , 2012, Nature Communications.