Dirofilaria immitis: the moulting of the infective larva in vitro

Abstract A number of in vitro culture systems were tested for their ability to support the development of Dirofilaria immitis infective larvae to the fourth larval stage. In cultures of medium ML-15 containing a feeder layer of Dog Sarcoma (DS) cells larvae successfully moulted and showed a small but significant increase in length. Ultrastructural observations demonstrated that the fourth-stage cuticle was synthesized in vitro and in some larvae was fully formed by 60 hours of culture. The hypodermis of moulting larvae contained numerous multi-vesicular bodies. It is concluded that the moult in vitro is a true moult and not an atypical response of the larvae to the conditions of culture.

[1]  R. Howells,et al.  The moulting process and the phenomenon of intermoult growth in the filarial nematode Brugia pahangi , 1983, Parasitology.

[2]  E. Franke,et al.  Dipetalonema viteae (Nematoda: Filarioidea): culture of third-stage larvae to young adults in vitro. , 1983, Science.

[3]  M. Wada,et al.  In vitro cultures of infective-stage larvae of Dirofilaria immitis and Brugia pahangi. , 1982, Annals of Tropical Medicine and Parasitology.

[4]  P. K. Lim,et al.  In vitro cultivation of Brugia malayi and Brugia pahangi infective larvae to fourth and fifth stage. , 1982, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[5]  T. Sawyer.  Molting and exsheathment in vitro of third-stage Dirofilaria immitis. , 1965, The Journal of parasitology.

[6]  A. Berntzen.  COMPARATIVE GROWTH AND DEVELOPMENT OF TRICHINELLA SPIRALIS IN VITRO AND IN VIVO, WITH A REDESCRIPTION OF THE LIFE CYCLE. , 1965, Experimental parasitology.

[7]  M. Yoeli,et al.  STUDIES ON FILARIASIS. 3. PARTIAL GROWTH OF THE MAMMALIAN STAGES OF DIROFILARIA IMMITIS IN VITRO. , 1964, Experimental parasitology.

[8]  R. Sommerville,et al.  THE INFECTIVE STAGE OF NEMATODE PARASITES AND ITS SIGNIFICANCE IN PARASITISM. , 1963, Advances in parasitology.

[9]  T. C. Orihel.  Morphology of the larval stages of Dirofilaria immitis in the dog. , 1961, The Journal of parasitology.

[10]  A. Taylor.  Maintenance of filarial worms in vitro. , 1960, Experimental parasitology.

[11]  R. O. Hayes.  Determination of a Physiological Saline Solution for Aedes aegypti (L.) , 1953 .