The transcription factor MebHLH18 in cassava functions in decreasing low temperature-induced leaf abscission to promote low-temperature tolerance

The reactive oxygen species (ROS) signal regulates stress-induced leaf abscission in cassava. The relationship between the function of the cassava transcription factor bHLH gene and low temperature-induced leaf abscission is still unclear. Here, we report that MebHLH18, a transcription factor, involved in regulating low temperature-induced leaf abscission in cassava. The expression of the MebHLH18 gene was significantly related to low temperature-induced leaf abscission and POD level. Under low temperatures, the levels of ROS scavengers in different cassava genotypes were significantly different in the low temperature-induced leaf abscission process. Cassava gene transformation showed that MebHLH18 overexpression significantly decreased the low temperature-induced leaf abscission rate. Simultaneously, interference expression increased the rate of leaf abscission under the same conditions. ROS analysis showed a connection between the decrease in the low temperature-induced leaf abscission rate caused by MebHLH18 expression and the increase in antioxidant activity. A Genome-wide association studies analysis showed a relationship between the natural variation of the promoter region of MebHLH18 and low temperature-induced leaf abscission. Furthermore, studies showed that the change in MebHLH18 expression was caused by a single nucleotide polymorphism variation in the promoter region upstream of the gene. The high expression of MebHLH18 led to a significant increase in POD activity. The increased POD activity decreased the accumulation of ROS at low temperatures and the rate of leaf abscission. It indicates that the natural variation in the promoter region of MebHLH18 increases antioxidant levels under low temperatures and slows down low temperature-induced leaf abscission.

[1]  Shuxia Li,et al.  Natural variation MeMYB108 associated with tolerance to stress-induced leaf abscission linked to enhanced protection against reactive oxygen species in cassava , 2022, Plant Cell Reports.

[2]  Li Chen,et al.  Map-based cloning and promoter variation analysis of the lobed leaf gene BoLMI1a in ornamental kale (Brassica oleracea L. var. acephala) , 2021, BMC plant biology.

[3]  Li Chen,et al.  Map-based cloning and promoter variation analysis of the lobed leaf gene BoLMI1a in ornamental kale (Brassica oleracea L. var. acephala) , 2021, BMC Plant Biology.

[4]  Z. Wen,et al.  Genome-wide identification and expression analysis of bHLH transcription factor family in response to cold stress in sweet cherry (Prunus avium L.) , 2021 .

[5]  Yingting Zhang,et al.  Cloning and cold-resistance analyses of CfICE1 gene in Cryptomeria fortunei. , 2021, Plant physiology and biochemistry : PPB.

[6]  Wen Feng Chen,et al.  Overexpression of RmICE1, a bHLH transcription factor from Rosa multiflora, enhances cold tolerance via modulating ROS levels and activating the expression of stress-responsive genes , 2020 .

[7]  Jie Luo,et al.  Natural variation in the OsbZIP18 promoter contributes to branched-chain amino acid levels in rice. , 2020, The New phytologist.

[8]  Q. Qian,et al.  Natural variation in the promoter of TGW2 determines grain width and weight in rice. , 2020, The New phytologist.

[9]  Jietang Zhao,et al.  DlICE1, a stress-responsive gene from Dimocarpus longan, enhances cold tolerance in transgenic Arabidopsis. , 2019, Plant physiology and biochemistry : PPB.

[10]  P. Yao,et al.  Overexpression of Fagopyrum tataricum FtbHLH2 enhances tolerance to cold stress in transgenic Arabidopsis. , 2018, Plant physiology and biochemistry : PPB.

[11]  Ji-Hong Liu,et al.  The transcription factor CsbHLH18 of sweet orange functions in modulation of cold tolerance and homeostasis of reactive oxygen species by regulating the antioxidant gene , 2018, Journal of experimental botany.

[12]  Zhaoquan Yao,et al.  Multi-Stimuli-Responsive Fluorescence Switching from a Pyridine-Functionalized Tetraphenylethene AIEgen. , 2018, ACS applied materials & interfaces.

[13]  Shuang Wu,et al.  NOG1 increases grain production in rice , 2017, Nature Communications.

[14]  M. Peng,et al.  Genome-wide identification of cassava R2R3 MYB family genes related to abscission zone separation after environmental-stress-induced abscission , 2016, Scientific Reports.

[15]  Jin-jian Lu,et al.  Psoralidin induced reactive oxygen species (ROS)-dependent DNA damage and protective autophagy mediated by NOX4 in breast cancer cells. , 2016, Phytomedicine : international journal of phytotherapy and phytopharmacology.

[16]  M. Yamasaki,et al.  Genome-wide association study using whole-genome sequencing rapidly identifies new genes influencing agronomic traits in rice , 2016, Nature Genetics.

[17]  Colin A. Chavel,et al.  Spatial landmarks regulate a Cdc42-dependent MAPK pathway to control differentiation and the response to positional compromise , 2016, Proceedings of the National Academy of Sciences.

[18]  M. Peng,et al.  Reactive oxygen species regulate leaf pulvinus abscission zone cell separation in response to water-deficit stress in cassava , 2016, Scientific Reports.

[19]  G. Bryan,et al.  Coping with drought: stress and adaptive responses in potato and perspectives for improvement , 2015, Front. Plant Sci..

[20]  P. Alam,et al.  A NOVEL ACYCLIC DITERPENIC ALCOHOL ISOLATED FROM ANTIOXIDANT ACTIVE ETHANOL EXTRACT OF LEAVES OF CENTAUROTHAMNUS MAXIMUS GROWN IN SAUDI ARABIA , 2015, African Journal of Traditional, Complementary and Alternative Medicines.

[21]  Eric A. Johnson,et al.  Rapid identification and recovery of ENU-induced mutations with next-generation sequencing and Paired-End Low-Error analysis , 2015, BMC Genomics.

[22]  Weixiong Zhang,et al.  Cassava genome from a wild ancestor to cultivated varieties , 2014, Nature Communications.

[23]  Sin-Ho Jung.  Stratified Fisher's exact test and its sample size calculation , 2014, Biometrical journal. Biometrische Zeitschrift.

[24]  Jun Yang,et al.  Coupled expression of Cu/Zn-superoxide dismutase and catalase in cassava improves tolerance against cold and drought stresses , 2013, Plant signaling & behavior.

[25]  Jun Yang,et al.  Enhanced Reactive Oxygen Species Scavenging by Overproduction of Superoxide Dismutase and Catalase Delays Postharvest Physiological Deterioration of Cassava Storage Roots1[C][W][OA] , 2013, Plant Physiology.

[26]  R. Kohli,et al.  Cell phone electromagnetic field radiations affect rhizogenesis through impairment of biochemical processes , 2012, Environmental Monitoring and Assessment.

[27]  Meng Li,et al.  Genome-wide association studies of 14 agronomic traits in rice landraces , 2010, Nature Genetics.

[28]  F. Van Breusegem,et al.  Reactive oxygen species as signals that modulate plant stress responses and programmed cell death , 2006, BioEssays : news and reviews in molecular, cellular and developmental biology.

[29]  J. Blaustein,et al.  Production of Reactive Oxygen Species by Plant NADPH Oxidases1 , 2006, Plant Physiology.

[30]  Lee,et al.  Chilling stress-induced changes of antioxidant enzymes in the leaves of cucumber: in gel enzyme activity assays. , 2000, Plant science : an international journal of experimental plant biology.