Platelet/polymorphonuclear leukocyte interaction in dynamic conditions: evidence of adhesion cascade and cross talk between P-selectin and the beta 2 integrin CD11b/CD18.

Adhesion between platelets and polymorphonuclear leukocytes (PMN) is a key event in thrombosis and inflammation. Double color fluorescence-activated cell sorter (FACS) analysis was used to determine the extent and kinetics of adhesion of thrombin-activated platelets to resting or activated PMN when mixed cell populations were incubated in dynamic conditions. Activated platelets bound very rapidly to PMN. Mixed cell conjugates reached a maximum at 1 minute and were reversible within 10 minutes. Platelet/PMN adhesion required both Ca2+ and Mg2+ and was markedly increased by the presence of Mn2+. The latter made mixed cell conjugates stable up to 10 minutes. Adhesion of platelets required metabolic activity of PMN and was abolished by tyrosine kinase inhibitors. Furthermore, adhesion of platelets to PMN resulted in binding of a monoclonal antibody (MoAb 24) known as beta 2 integrins "activation reporter." When PMN were activated by exogenous stimuli, the adhesion of platelets was markedly increased: fMLP induced a rapid and transient effect, while PMA resulted in a slower, but stable, increase in mixed conjugates formation. The hypothesis that activated PMN beta 2 integrins are able to bind a counter-receptor on platelets was directly demonstrated by the increase of mixed cell conjugates following PMN treatment with KIM127 and KIM185, two anti-CD18 antibodies able to induce the active conformation of beta 2 integrins. Consistently, two other anti-CD18, as well as an anti-CD11b inhibitory antibody abolished platelet/PMN adhesion. PMN beta 2 integrin activation was not the only mechanism for activated platelet/PMN adhesion to occur: indeed, this phenomenon could also be inhibited by two anti-P-selectin antibodies. Resting platelets did not adhere to resting PMN, but markedly adhered to fMLP- or PMA-activated PMN. Resting platelet/fMLP-activated PMN adhesion was abolished by anti-CD18 antibodies, but not by anti-P-selectin antibodies. In conclusion, activated platelet/PMN interaction can be modeled as an adhesion cascade involving a P-selectin-dependent recognition step and a functional signal. The latter proceeds through tyrosine kinase activation and enables a beta 2 integrin-dependent adhesion to a not yet identified counter-receptor constitutively expressed on platelet surface.

[1]  G. Nash,et al.  Continuous activation and deactivation of integrin CD11b/CD18 during de novo expression enables rolling neutrophils to immobilize on platelets. , 1996, Blood.

[2]  L. Koenderman,et al.  Platelet-dependent primary hemostasis promotes selectin- and integrin-mediated neutrophil adhesion to damaged endothelium under flow conditions. , 1996, Blood.

[3]  A. Weyrich,et al.  Activated platelets signal chemokine synthesis by human monocytes. , 1996, The Journal of clinical investigation.

[4]  H. Patscheke,et al.  Platelet‐induced neutrophil activation: platelet‐expressed fibrinogen induces the oxidative burst in neutrophils by an interaction with CD11C/CD18 , 1995, British journal of haematology.

[5]  A. Marcus,et al.  Thrombosis and Inflammation as Multicellular Processes: Significance of Cell-Cell Interactions , 1995, Thrombosis and Haemostasis.

[6]  G. Zimmerman,et al.  Activation of polymorphonuclear leukocytes reduces their adhesion to P-selectin and causes redistribution of ligands for P-selectin on their surfaces. , 1995, The Journal of clinical investigation.

[7]  C. Cerletti,et al.  Platelet Activation by Polymorphonuclear Leukocytes: Role of Cathepsin G and P-Selectin , 1995, Thrombosis and Haemostasis.

[8]  T. Cai,et al.  Energetics of Leukocyte Integrin Activation (*) , 1995, The Journal of Biological Chemistry.

[9]  C. Gahmberg,et al.  A peptide derived from the intercellular adhesion molecule-2 regulates the avidity of the leukocyte integrins CD11b/CD18 and CD11c/CD18 , 1995, The Journal of cell biology.

[10]  A. Weyrich,et al.  Monocyte tethering by P-selectin regulates monocyte chemotactic protein-1 and tumor necrosis factor-alpha secretion. Signal integration and NF-kappa B translocation. , 1995, The Journal of clinical investigation.

[11]  D. Hammer,et al.  Lifetime of the P-selectin-carbohydrate bond and its response to tensile force in hydrodynamic flow , 1995, Nature.

[12]  W. Muller,et al.  Ligation of platelet/endothelial cell adhesion molecule 1 (PECAM-1/CD31) on monocytes and neutrophils increases binding capacity of leukocyte CR3 (CD11b/CD18). , 1995, Journal of immunology.

[13]  Joseph F. Murphy,et al.  The vitronectin receptor (alpha v beta 3) is implicated, in cooperation with P-selectin and platelet-activating factor, in the adhesion of monocytes to activated endothelial cells. , 1994, The Biochemical journal.

[14]  B. Smith,et al.  Neutrophil but not Monocyte Activation Inhibits P-Selectin-Mediated Platelet Adhesion , 1994, Thrombosis and Haemostasis.

[15]  T. Carlos,et al.  Leukocyte-endothelial adhesion molecules. , 1994, Blood.

[16]  M. Vadas,et al.  P-selectin interacts with a beta 2-integrin to enhance phagocytosis. , 1994, Journal of immunology.

[17]  G. Pellegrini,et al.  P-selectin induces the expression of tissue factor on monocytes. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[18]  K. Messmer,et al.  P-selectin mediates the interaction of circulating leukocytes with platelets and microvascular endothelium in response to oxidized lipoprotein in vivo. , 1994, Laboratory investigation; a journal of technical methods and pathology.

[19]  C. Cerletti,et al.  Polymorphonuclear Leukocyte-Platelet Interaction: Role of P-Selectin in Thromboxane B2 and Leukotriene C4 Cooperative Synthesis , 1994, Thrombosis and Haemostasis.

[20]  T. Springer,et al.  A functional integrin ligand on the surface of platelets: intercellular adhesion molecule-2. , 1994, The Journal of clinical investigation.

[21]  H. Lehr,et al.  Vitamin C prevents cigarette smoke-induced leukocyte aggregation and adhesion to endothelium in vivo. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[22]  A. Varki,et al.  Selectin ligands. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[23]  P. Kubes,et al.  Regulation of stimulated integrin surface expression in human neutrophils by tyrosine phosphorylation. , 1994, Blood.

[24]  D. Altieri,et al.  Differential ligand binding specificities of recombinant CD11b/CD18 integrin I-domain. , 1994, The Journal of biological chemistry.

[25]  B. Furie,et al.  Expression cloning of a functional glycoprotein ligand for P-selectin , 1993, Cell.

[26]  T. Irimura,et al.  Activated platelets induce superoxide anion release by monocytes and neutrophils through P-selectin (CD62). , 1993, Journal of immunology.

[27]  B. Kehrel,et al.  The Platelet Glycoprotein IIb/IIIa Complex Is lnvolved in the Adhesion of Activated Platelets to Leukocytes , 1993, Thrombosis and Haemostasis.

[28]  G. Nash,et al.  Selectin-mediated rolling of neutrophils on immobilized platelets. , 1993, Blood.

[29]  L. Languino,et al.  Fibrinogen mediates leukocyte adhesion to vascular endothelium through an ICAM-1-dependent pathway , 1993, Cell.

[30]  N. Hogg,et al.  Ligand intercellular adhesion molecule 1 has a necessary role in activation of integrin lymphocyte function-associated molecule 1. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[31]  J. White,et al.  Cathepsin G-dependent platelet stimulation by activated polymorphonuclear leukocytes and its inhibition by antiproteinases: role of P-selectin-mediated cell-cell adhesion. , 1993, Blood.

[32]  D. Altieri,et al.  The structural motif glycine 190-valine 202 of the fibrinogen gamma chain interacts with CD11b/CD18 integrin (alpha M beta 2, Mac-1) and promotes leukocyte adhesion. , 1993, The Journal of biological chemistry.

[33]  C. Benjamin,et al.  Leukocyte accumulation promoting fibrin deposition is mediated in vivo by P-selectin on adherent platelets , 1992, Nature.

[34]  E. Dejana,et al.  Platelet-dependent modulation of neutrophil function. , 1992, Pharmacological research.

[35]  C. Cerletti,et al.  Polymorphonuclear leucocyte-dependent modulation of platelet function: relevance to the pathogenesis of thrombosis. , 1992, Pharmacological research.

[36]  R. Cummings,et al.  Identification of a specific glycoprotein ligand for P-selectin (CD62) on myeloid cells , 1992, The Journal of cell biology.

[37]  E. Butcher,et al.  Antibody against the Leu-CAM beta-chain (CD18) promotes both LFA-1- and CR3-dependent adhesion events. , 1992, Journal of immunology.

[38]  B. Smith,et al.  Activated and unactivated platelet adhesion to monocytes and neutrophils. , 1991, Blood.

[39]  J. White,et al.  Platelet activation by fMLP-stimulated polymorphonuclear leukocytes: the activity of cathepsin G is not prevented by antiproteinases. , 1991, Blood.

[40]  T. Springer,et al.  Leukocytes roll on a selectin at physiologic flow rates: Distinction from and prerequisite for adhesion through integrins , 1991, Cell.

[41]  B. Catimel,et al.  Inhibition of platelet functions by a monoclonal antibody (LYP20) directed against a granule membrane glycoprotein (GMP-140/PADGEM). , 1991, Blood.

[42]  A. Varki,et al.  GMP-140 binds to a glycoprotein receptor on human neutrophils: evidence for a lectin-like interaction , 1991, The Journal of cell biology.

[43]  J. Loike,et al.  CD11c/CD18 on neutrophils recognizes a domain at the N terminus of the A alpha chain of fibrinogen. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[44]  S. Simon,et al.  Flow cytometric analysis and modeling of cell-cell adhesive interactions: the neutrophil as a model , 1990, The Journal of cell biology.

[45]  A. Marcus,et al.  Stratton lecture 1989. Thrombosis and inflammation as multicellular processes: pathophysiologic significance of transcellular metabolism. , 1990, Blood.

[46]  T. Kuijpers,et al.  Distinct adhesive properties of granulocytes and monocytes to endothelial cells under static and stirred conditions. , 1990, Journal of immunology.

[47]  D. Altieri,et al.  A unique recognition site mediates the interaction of fibrinogen with the leukocyte integrin Mac-1 (CD11b/CD18). , 1990, The Journal of biological chemistry.

[48]  E. Chi,et al.  Inhibition of leukocyte adherence by anti-CD18 monoclonal antibody attenuates reperfusion injury in the rabbit ear. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[49]  R. McEver,et al.  GMP-140 mediates adhesion of stimulated platelets to neutrophils. , 1990, Blood.

[50]  R. Geha,et al.  Constitutive and stimulus-induced phosphorylation of CD11/CD18 leukocyte adhesion molecules , 1989, The Journal of cell biology.

[51]  D. Wagner,et al.  PADGEM protein: A receptor that mediates the interaction of activated platelets with neutrophils and monocytes , 1989, Cell.

[52]  L. Aarden,et al.  Regulatory properties of LFA-1 α and β chains in human T-lymphocyte activation , 1988, Nature.

[53]  P. J. Simpson,et al.  Reduction of experimental canine myocardial reperfusion injury by a monoclonal antibody (anti-Mo1, anti-CD11b) that inhibits leukocyte adhesion. , 1988, The Journal of clinical investigation.

[54]  U. Nydegger,et al.  Platelet-leukocyte interaction: selective binding of thrombin-stimulated platelets to human monocytes, polymorphonuclear leukocytes, and related cell lines. , 1986, Blood.

[55]  E. Dejana,et al.  Platelet Adhesion to Subendothelium - Effect of Shear Rate, Hematocrit and Platelet Count on the Dynamic Equilibrium Between Platelets Adhering to and Detaching from the Surface , 1985, Thrombosis and Haemostasis.

[56]  H. Ochs,et al.  The role of neutrophil membrane glycoprotein GP-150 in neutrophil adherence to endothelium in vitro. , 1985, Blood.

[57]  W. V. Voorhis,et al.  Identification of the C3bi receptor of human monocytes and macrophages by using monoclonal antibodies. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[58]  J. Strominger,et al.  Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[59]  D. Hammer,et al.  Lifetime of the P-selectin-carbohydrate bond and its response to tensile force in hydrodynamic flow , 1995, Nature.

[60]  I. Feuerstein,et al.  Role of P-selectin and leukocyte activation in polymorphonuclear cell adhesion to surface adherent activated platelets under physiologic shear conditions (an injury vessel wall model). , 1994, Blood.

[61]  L. Lindbom,et al.  A monoclonal antibody to the membrane glycoprotein complex CD18 inhibits polymorphonuclear leukocyte accumulation and plasma leakage in vivo , 1987 .

[62]  J. Loike,et al.  CD 11 c / CD 18 on neutrophils recognizes a domain at the N terminus of the Aa chain of fibrinogen , 2022 .

[63]  Rui,et al.  A Peptide Derived from the Intercellular Adhesion Molecule-2 Regulates the Avidity of the Leukocyte Integrins CD 11 b / CD 18 and CDllc / CD 18 , 2022 .