A Comparative Study of Gene Microarray between Normal and Diabetic Keratocyte in Rat Cornea

Purpose: To differentiate the genes that are up-regulated or down-regulated and the cellular morphology in the corneal stromal cells between normal and diabetic rat. Methods: After isolation with trizol (GIBCO, NY, USA), labeling and purification of complementary DNA(cDNA) probes were carried out using Glass Microarrays 1.0. Results: Autoradiographic analysis showed that 35 genes were up-regulated and 8 genes were down-regulated by Atlas ImageTM Software. Up-regulated genes were as calcium/calmodulin dependent protein kinase I, proteasome 26S subunit 1, prostaglandin F receptor. Down-regulated genes were aquaporin 8, cytochrome c oxidase polypeptide Vb, glypican 1. Conclusions: There were many differential genes in hormone receptor, voltage gated ion channels, oncogenes & tumor suppressors, G protein between normal and diabetic keratocyte of rat.